Molecular marker for Fenneropenaeus chinensis with high-pH resistance character and identification method using same

Abstract

The invention provides a molecular marker for Fenneropenaeus chinensis with a high-pH resistance character and an identification method using the same. The polymerase chain reaction (PCR) identification method for the high-pH resistance character of Fenneropenaeus chinensis is established based on a specific sequence responsible for the high-pH resistance character of Fenneropenaeus chinensis and designed specific primers, and the specific sequence responsible for the high-pH resistance character of Fenneropenaeus chinensis is obtained by SRAP (sequence-related amplified polymorphism) marker screening, cloning, sequencing, specific primer design and specific marker verification. The method for screening the specific SRAP marker to the high-pH resistance character of Fenneropenaeus chinensis is established to select one primer pair composition from 110 SRAP primer pair compositions to produce a high-pH resistance specific SRAP fragment. The PCR identification method for the high-pH resistance character of Fenneropenaeus chinensis is established for the first time. The identification method is advanced, stable and reliable; and has significance and wide prospects in application for stress resistance breeding of Fenneropenaeus chinensis, enhancement of environmental adaptability of Fenneropenaeus chinensis, and large-area farming of Fenneropenaeus chinensis.

Description

technical field [0001] The invention belongs to the field of genetic engineering of aquatic biotechnology, and relates to molecular markers and genetic identification technology related to shrimp tolerance to high pH, ​​in particular to a molecular marker and identification method of Chinese prawn tolerance to high pH. Background technique [0002] Chinese prawns ( Fenneropenaeus chinensis ) Natural resources are mainly distributed in my country's Yellow Sea, Bohai Sea and the coast of the Korean Peninsula. In the early 1990s, the output reached more than 200,000 tons, ranking first in the world for many years. Among them, the production of prawn farming in China accounted for more than 70% of the total production of prawn farming in the country. Landslides, the annual output has been hovering around 50,000 tons for a long time, and the resulting economic losses have reached tens of billions of yuan. During the breeding process, the growth rate slowed down and the ability ...

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Problems solved by technology

This technique has been widely used in plant genetic diversity analysis (Budak H, 2004), hybrid vigor estimation (Riaz A, 2001), comparative genomics research (Li G, 2003), genetic linkage map construction (Lin Z X, 2009) and related gene mapping (Alwala, 2009), but there are few research applications in the field of aquatic animal research, only in Macrobrachium rosenbergii (Macrobrachium Rosenbergii ) (Zhou Jinsong, 2006), Macrobrachium japonica ( Macrobrachium nipponensis ) (Yao Jianhua, 2008) and grass carp ( Ctenopharyngodon idellus ) (Zhang Zhiwei, 2007; Ding Weidong, 2008) have seen reports

[0005] Traditional breeding methods mainly use phenotypic differences to selec

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