Method for preparing corn peptide

A technology of corn peptide and corn gluten powder, which is applied in the field of preparation of plant protein composition, can solve the problems of ineffective production of corn peptide and achieve the effect of improving utilization rate

Active Publication Date: 2011-09-07
武汉百信正源生物技术工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, this method is not an efficient method for producing corn peptides

Method used

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  • Method for preparing corn peptide

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Embodiment 1

[0037] A preparation method of corn peptide, the method comprises the steps of:

[0038](A) Take 50g of corn gluten powder, add it to 2L of 90% ethanol solution, stir, heat the solution to 55°C, adjust the pH to 10.0 with NaOH solution, add 5g of alkaline protease, and in the alcohol phase under this condition Hydrolyze for 2 hours, use NaOH solution to keep the pH value at 10.0 during the enzymolysis process;

[0039] (B) After step (A), evaporate 1L of ethanol solution, add water to make up to the volume of the original hydrolyzate, stir evenly, heat the solution to 45°C, adjust the pH to 10.0 with NaOH solution, add 2g of compound enzyme, press here Hydrolyze in the alcohol phase for 20 minutes under the same conditions, and use NaOH solution to keep the pH value at 10.0 during the enzymolysis process;

[0040] (C) After step (B), adjust the pH to 3.0 with HCl solution, and centrifuge for 20 to 30 minutes at a centrifuge speed of 3000 r / m to obtain a supernatant, which is ...

Embodiment 2

[0048] A preparation method of corn peptide, the method comprises the steps of:

[0049] (A) Take 100g of corn gluten powder, add it to 2L of 80% ethanol solution, stir, heat the solution to 45°C, adjust the pH to 9.0 with NaOH solution, add 9g of alkaline protease, and use this condition in the alcohol phase Hydrolyze for 3 hours, and use NaOH solution to keep the pH value at 9.0 during the enzymolysis process;

[0050] (B) After step (A), evaporate 1000mL of ethanol solution, add water to make up the volume of the original hydrolyzate, stir evenly, heat the solution to 45°C, adjust the pH to 8.0 with NaOH solution, add 5g of compound enzyme, press here Hydrolyze in the alcohol phase for 30 minutes under the same conditions, and use NaOH solution to keep the pH value at 8.0 during the enzymolysis process;

[0051] (C) After step (B), adjust the pH to 4.5 with HCl solution, centrifuge at 3000 rpm for 15 minutes to obtain the supernatant, which is the crude corn peptide;

[0...

Embodiment 3

[0059] A preparation method of corn peptide, the method comprises the steps of:

[0060] (A) Take 500g of corn gluten powder, add it to 15L of 85% ethanol solution, stir, heat the solution to 50°C, adjust the pH to 8.5 with NaOH solution, add 50g of alkaline protease, and in the alcohol phase under this condition Hydrolyze for 3 hours, and use NaOH solution to keep the pH value at 8.5 during the enzymolysis process;

[0061] (B) After step (A), evaporate 10L of ethanol solution, add water to make up to the volume of the original hydrolyzate, stir evenly, heat the solution to 50°C, adjust the pH to 8.5 with NaOH solution, add 15g of compound enzyme, press here Hydrolyze in the alcohol phase for 40 minutes under conditions, and use NaOH solution to keep the pH value at 8.5 during the enzymolysis process;

[0062] (C) After step (B), adjust the pH to 4.0 with HCl solution, centrifuge at 3000 rpm for 15 minutes, and obtain the supernatant, which is the corn peptide crude liquid; ...

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Abstract

The invention relates to a method for preparing a plant protein composition, in particular to a method for preparing corn peptide. The method comprises the following processes of: adding ethanol into corn protein powder, performing enzymolysis in an alcohol phase, evaporating partial ethanol and adding equivalent water, adding complex enzyme, continuously performing enzymolysis in the aqueous phase, centrifuging the hydrolysis solution, performing ultra-filtration classification and anion cation exchange desalination, finally concentrating the filtrate, and drying the concentrate to obtain dry corn peptide powder. The molecular weight of the prepared corn peptide is mainly centralized between 600 and 3,000, and the corn peptide has good sobering function.

Description

technical field [0001] The invention relates to a preparation method of a plant protein composition, in particular to a preparation method of corn peptide. Background technique [0002] Peptide is a compound composed of amino acids connected by peptide bonds, and it is the basic component of tissue cells in the body. The simplest peptide is a dipeptide composed of two amino acids, which contains 1 peptide bond; peptides containing 3, 4, 5, etc. amino acids are called tripeptides, tetrapeptides, pentapeptides, etc., respectively. Straight bond peptides formed by 2 to 10 amino acids through peptide bonds are called oligopeptides or small peptides; polymers of more than 10 amino acids combined by peptide bonds are called polypeptides. Scientists have discovered more than 100 biologically active peptides in the human body. These peptides have the function of transmitting physiological information and regulating physiological functions. Maintenance is very important. The occurr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K2/00C07K1/36C07K1/34C07K1/18A61P25/32
Inventor 陈燕飞亓培实何东平刘良忠何莉萍江思佳黄迪惠倪倩刘雪姣宋洋
Owner 武汉百信正源生物技术工程有限公司
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