Small peptides extracted from internal organs of squids, method for preparing same, composition thereof and use thereof as protein source of marine aquatic feed

Abstract

The invention relates to small peptides extracted from internal organs of squids and a method for preparing the same. The small peptides are characterized by containing an amino acid structural fragment-ILGGSDPKHYTG- with the relative molecular weight of between 1,400 and 5,800. The small peptides are prepared by the following steps: performing enzymolysis of internal organs of Japanese squids, filtering by using an ultrafiltration membrane of which the intercepted molecular weight is 18,000Da, separating and purifying by using gel chromatography to obtain the small peptide matters containingthe amino acid structural fragment-ILGGSDPKHYTG-, adding potassium sorbate, uniformly stirring and drying. The invention also relates to a composition consisting of the following ingredients: 6 to 8 percent of small peptides extracted from internal organs of squids, 50 to 60 percent of soya bean meal, 3 to 6 percent of fish meal, 27 to 33 percent of corn flour and 2 to 5 percent of starch for granulation. The composition can be used as a protein source of a feed for marine fishes, shrimps, shells and the like, particularly the protein source of the feed for marine fishes. If the marine fishesare fed by the composition, the feeding amount of the marine fishes is greatly improved, and the unit weight of the marine fishes is raised.

Description

technical field [0001] The present invention relates to a new small peptide substance, in particular to a small peptide extracted from squid viscera and its preparation method, and also to the composition of this substance and its use as a feed protein source in marine aquaculture use. Background technique [0002] With the rapid development of my country's distant water fishery, the catch of squid has increased year by year. In 2009, the catch of squid has exceeded 300,000 tons, and it has become the main raw material for aquatic product processing in my country. But my country's squid processing technology content is low, after processing and processing to obtain products such as fish flower, dried fish, salty squid, canned food and condiments, generally have 20%~30% waste (viscera, head, foot, epidermis and skin) Ink, etc.), these wastes are rich in nutrients such as protein, fat, polysaccharides, etc., according to Wu Limin ("Agricultural Products Processing", 2007 (8): ...

AI Technical Summary

Problems solved by technology

[0004] Since the existing production process of feed protein source is mainly obtained by high-temperature cooking and concentration after enzymatic hydrolysis, these processes are easy to denature protein and produce collagen, which not only reduces the nutritional content, but also greatly damages the product’s impact on aquatic animals. Attractive

Even with the method of Su Zufeng et al., using SJN2-2000 two-effect evaporator to concentrate the enzymatic solution twice, so that the evaporation temperature is controlled below 90°C, which greatly reduces the concentration temperature, but it still cannot fundamentally solve this problem.

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