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Extraction method of DHA (docosahexaenoic acid) algae oil by biological fermentation

An extraction method and technology of algae oil, which is applied in the directions of fermentation, fat production, bulk chemical production, etc., can solve the problems of long extraction process route, complicated process and high energy consumption, and achieve short process route, simple process and low energy consumption Effect

Active Publication Date: 2013-03-06
TSINGDAO BIOFORCE AID TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method does not need to use organic solvents for extraction, and the obtained DHA product is not easily polluted by organic solvents. However, this method needs to first break the wall of biological enzymes, and then perform five-stage molecular distillation. The entire extraction process is long, complicated, and energy efficient. high power consumption

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  • Extraction method of DHA (docosahexaenoic acid) algae oil by biological fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Drying the solid obtained by solid-liquid separation of the Schizochytrium fermented liquid to obtain dried bacteria;

[0056] Add 100g of the dry thalline to the extraction tank, spray 5g of absolute ethanol on the dry thalline and close the extraction tank; open the gas storage tank, keep the flow of carbon dioxide at 4.8L / h, and the carbon dioxide passes through the condenser, high-pressure After the pump and the preheater, it enters the extraction tank for extraction under the conditions of 20MPa and 50°C, and the extracted carbon dioxide fluid enters the first separator for first-stage separation under the conditions of 10MPa and 45°C to obtain the second One part of algae oil; the carbon dioxide fluid after the first-stage separation continues to enter the second separator, and the second-stage separation is carried out under the conditions of 6MPa and 40°C to obtain the second part of algae oil.

[0057] After dynamic extraction for 2 hours, a total of 34.09g DHA...

Embodiment 2

[0059] Drying the solid obtained by solid-liquid separation of the Schizochytrium fermented liquid to obtain dried bacteria;

[0060] Add 100g of the dry thalline to the extraction tank, spray 4g of absolute ethanol on the dry thalline and close the extraction tank; open the gas storage tank, keep the flow of carbon dioxide at 4.8L / h, and the carbon dioxide passes through the condenser, high pressure After the pump and the preheater, it enters the extraction tank and extracts under the conditions of 20MPa and 50°C. The extracted carbon dioxide fluid enters the first separator and performs the first stage separation under the conditions of 9MPa and 50°C to obtain the second One part of algae oil; the carbon dioxide fluid after the first-stage separation continues to enter the second separator, and the second-stage separation is carried out under the conditions of 5MPa and 30°C to obtain the second part of algae oil.

[0061] After dynamic extraction for 2 hours, a total of 33.6...

Embodiment 3

[0063] Drying the solid obtained by solid-liquid separation of the Schizochytrium fermented liquid to obtain dried bacteria;

[0064] Add 100g of the dry thalline to the extraction tank, spray 4g of absolute ethanol on the dry thalline and close the extraction tank; open the gas storage tank, keep the flow of carbon dioxide at 4.8L / h, and the carbon dioxide passes through the condenser, high pressure After the pump and the preheater, it enters the extraction tank and extracts under the conditions of 18MPa and 45°C. The extracted carbon dioxide fluid enters the first separator and performs the first stage separation under the conditions of 12MPa and 45°C to obtain the second One part of algae oil; the carbon dioxide fluid after the first-stage separation continues to enter the second separator, and the second-stage separation is carried out under the conditions of 5MPa and 30°C to obtain the second part of algae oil.

[0065] After 2 hours of dynamic extraction, a total of 33.1...

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Abstract

The invention provides an extraction method of DHA (docosahexaenoic acid) algae oil by biological fermentation, which comprises the following steps: a) drying solids obtained after performing solid-liquid separation on microalgae fermentation broth so as to get dry thalli; b) extracting the dry thalli by taking supercritical carbon dioxide as an extraction agent so as to get carbon dioxide fluid; and c) performing decompression separation on the carbon dioxide fluid so as to get the DHA algae oil. The supercritical carbon dioxide is taken as the extraction agent for extracting the DHA algae oil, the extraction efficiency is high, and the obtained DHA algae oil has high purity, high yield and stable quality. Experiments prove that, in the DHA algae oil obtained by adopting the method, the content of DHA is more than 40%. Simultaneously, the method does not need to perform flocculation, biological enzyme wall breaking and other operations, the extraction can be directly performed after performing the solid-liquid separation and the drying on the microalgae fermentation broth, the DHA algae oil can be obtained after directly performing the decompression separation after completing the extraction, and the complex aftertreatment process does not need to be performed.

Description

technical field [0001] The invention belongs to the technical field of DHA algae oil, in particular to a method for extracting biologically fermented DHA algae oil. Background technique [0002] Docosahexaenoic acid, the English name is Docosahexaenoic Acid, referred to as DHA, the molecular formula is C 22 h 32 o 2 , is a long-chain Omega-3 polyunsaturated fatty acid. DHA is the main element for the growth and function maintenance of nervous system cells, and is an important component of the brain and retina. Studies have shown that the content of DHA in the human cerebral cortex is about 20%, and the content in the retina is as high as 50%. DHA can significantly promote the brain and vision development of infants, and a series of symptoms such as growth retardation and mental retardation can be caused when DHA is lacking. However, the human body synthesizes very little DHA and needs to obtain it from food. Therefore, it is particularly necessary to add DHA to food, esp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C11B1/10C11B1/02
CPCY02P20/54
Inventor 朱海刘卫斌
Owner TSINGDAO BIOFORCE AID TECH CO LTD