Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and application of active component of radix gentianae extractive

A technology of gentian extract and active components, which is applied in the field of medicinal use of traditional Chinese medicine to achieve significant therapeutic effects, reduce nerve cell death, and reduce brain edema

Inactive Publication Date: 2013-03-13
ZHEJIANG UNIV +1
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report about the activity of gentian active components similar to nerve growth factor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of active component of radix gentianae extractive
  • Preparation method and application of active component of radix gentianae extractive
  • Preparation method and application of active component of radix gentianae extractive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Preparation of n-GS

[0036] A preparation method for obtaining the active component n-GS with significant NGF mimic activity from the traditional Chinese medicine Gentiana, the specific steps are:

[0037] 1) Crushing and extraction:

[0038]After 1000 g of traditional Chinese medicine gentian was crushed, it was extracted with 5 L of ethanol (pharmaceutical grade) at room temperature for 5 days (occasionally shaking). After suction filtration and concentration, 138.98 g of crude ethanol extract was obtained, which was separated by alternating extraction with 80% aqueous methanol (750 ml) and n-hexane until the n-hexane layer was clear. A total of 13 g of crude n-hexane layer was obtained.

[0039] 2) Separation and purification:

[0040] The crude n-hexane layer was separated by a silica gel open column (200-300 mesh, the following solvent systems are all in volume ratio, n-hexane:chloroform=100:0, 90:10, 80:20, 70:30, 50:50 ), the desired active compo...

Embodiment 2

[0047] Example 2 n-GS has a protective effect on ischemic brain injury and can partially improve post-stroke dyskinesia

[0048] Main materials for the experiment: Kunming mice (40 males and 40 females each), weighing 25 g to 30 g, purchased from the Experimental Animal Center of Jiangsu Province.

[0049] Experimental operation: 10% chloral hydrate intraperitoneal anesthesia, ligation of the right common carotid artery and external carotid artery, clipping the distal end of the internal carotid artery with an arterial clip, inserting fishing line into the distal end of the internal carotid artery, and implementing the middle cerebral artery for 60 minutes Obstruction (middle cerebral artery occlusion is abbreviated as MCAO in the figure), and a mouse model of focal cerebral ischemia was prepared (use an electric blanket to keep the temperature of the anus of the experimental animal at 37±0.5 degrees). One hour after cerebral ischemia, n-GS was intraperitoneally injected at ...

Embodiment 3

[0051] Example 3 n-GS promotes neurogenesis in adults

[0052] Using BrdU (5-bromodeoxyuridine) to mark mitotic cells, it was found that the neural stem cells of the adult mammalian hippocampus can differentiate into nerve cells - nerve regeneration. Newborn neurons can establish synaptic connections with neurons in CA3 area and undergo synaptic plasticity. It is believed that the neural regeneration of the adult brain can replace neurons that die naturally due to aging or disease, and maintain the integrity of brain function and structure. This experiment was to observe the effect of PS on the process of nerve regeneration (mainly including the proliferation, survival and differentiation of neural stem cells).

[0053] Main materials for the experiment: 2-month-old Kunming mice (40 males and 40 females each), weighing 25g-30g, purchased from the Experimental Animal Center of Jiangsu Province.

[0054] Experimental operation: BrdU was injected three times continuously with...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an active radix gentianae component extracted from Chinese medicine radix gentianae. The active radix gentianae component is white, has pungent smell, and is easy to dissolve in chloroform, soluble in normal hexane and slightly soluble in alcohol and methanol. When TLC analysis is carried out, chloroform is used as a solvent, the Rf value of a 5-cm high forward silica plate is about 0.4, the sample can obviously absorb ultraviolet under 248nm to 320nm of wavelengths, and the content of the active component in dry radix gentianae roots and rhizomes is about 0.1%. The active radix gentianae component in the invention can be obtained by lixiviating an organic solvent, distributing a solvent and separating an open chromatographic column, has obvious similar nerve growth factor activity, can promote the rebirth of neurons of the adult brain and prevent the regenerating function of the neurotrosis and the inhibitory nerve of beta-amyloid, and can be applied to preparing medicaments for preventing and treating neurodegenerative diseases such as hypoxic ischemia brain damage, senile dementia and the like.

Description

technical field [0001] The invention belongs to the field of medicinal uses of traditional Chinese medicines, and specifically relates to an active component of an extract prepared from a Chinese medicinal material, gentiana, and its application in the preparation of medicines for treating ischemic brain injury and preventing and treating neurodegenerative diseases such as senile dementia. . Background technique [0002] Alzheimer's disease is mainly divided into two types: Alzheimer's disease (AD for short) and vascular dementia. (1) Cerebral ischemic disease is one of the most common clinical diseases, characterized by high morbidity, disability and mortality, which seriously threatens human health. With the gradual aging of the world's population, the incidence of ischemic brain injury is increasing year by year, but so far there is still a lack of effective prevention and treatment measures at home and abroad. The high sensitivity of central nervous cells to hypoxic in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/515A23L1/29A61P9/10A61P25/28A23L33/00
Inventor 戚建华陈玲向兰周丽彬曹时宁钱黎莉高丽娟李金优
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com