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Simple culture method of photosynthetic bacteria with high cell concentration

A technology for photosynthetic bacteria and cell concentration, which is applied in the fields of biotechnology and microbial applications, can solve the problems of limited popularization and application, rising ethanol prices, and high ethanol prices, and achieves the effects of strong maneuverability, reduced production costs, and simple equipment.

Inactive Publication Date: 2011-10-12
TIANJIN CITY AGRI BIO TECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

China Invention Patent Application No. 200910039524.0 "A Production Method for Photosynthetic Bacteria Aquaculture Feed Additives" can ensure the stable quality of the photosynthetic bacteria fermentation liquid produced, but it requires a fully automatic photosynthetic microorganism fermentation tank, and the output is also limited by the fermentation tank body , the high production cost will limit the popularization and application of this technology
Patent No. 03220904.5 "Vertical Photosynthetic Bacteria Cultivation Device" Although it saves a certain amount of space and can also use artificial light sources, the device needs to be specially customized and has poor mobility. If the use of sunlight requires a site similar to a glass house with strong light transmission , if the artificial light is fully utilized, the energy consumption will be more
From the analysis of the medium, the carbon source accounts for more than 50% of the dry weight of photosynthetic bacteria, so there are many studies on the carbon source of photosynthetic bacteria. Generally, sodium acetate is used as the main organic carbon source of the medium. Patent No. 200410009803.x" A method for cultivating photosynthetic bacteria with ultra-high cell concentration" uses ethanol instead of sodium acetate, and the cell concentration of the prepared photosynthetic bacteria can reach more than 4.5 billion per milliliter, but the price of ethanol is relatively high, and with the energy crisis Intensified, the price of ethanol will increase day by day

Method used

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  • Simple culture method of photosynthetic bacteria with high cell concentration
  • Simple culture method of photosynthetic bacteria with high cell concentration
  • Simple culture method of photosynthetic bacteria with high cell concentration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Determination of Photosynthetic Bacteria Medium and Culture Conditions

[0038] Photosynthetic bacteria medium (g / L): CH 3 COONa 3.0, NaHCO 3 8.0, NH 4 Cl 1.0, NaCl 1.0, MgSO 4 ·7H 2 O0.2,K 2 HPO 4 0.5, KH 2 PO 4 0.2, CaCl 2 0.05, yeast paste 0.5, pH natural.

[0039] Temperature: transfer the bacterium into the culture medium according to the inoculum amount of 10%, and culture them under anaerobic light (light intensity about 3000lux) at 15°C, 20°C, 25°C, 30°C, 35°C, and 40°C respectively. The number of bacteria was counted on the 7th day of the test, and the results are shown in Table 1.

[0040] Table 1 Effect of temperature on the growth of photosynthetic bacteria

[0041]

[0042] It can be seen from Table 1 that the growth of photosynthetic bacteria is good in the temperature range of 25-35°C, and the difference is not large. The optimum growth temperature of photosynthetic bacteria is 30°C, and the growth of photosynthetic bacteria is significant...

Embodiment 2

[0052] Indoor open culture test

[0053] The configuration method of the photosynthetic bacteria culture medium is the same as embodiment 1, the multi-column tower type photosynthetic bacteria culture device is cleaned, the bacterial classification of 20% is connected in the post, then adds 80% nutrient solution, gives artificial light source with the incandescent lamp of 100w, Adjust the distance of the light bulb so that the light intensity is about 3000lux, the temperature is controlled at about 30°C, and samples are taken every day to measure the concentration of the bacterial solution.

[0054] Table 4 The concentration of photosynthetic bacteria changes with time (10 8 pieces / ml)

[0055]

[0056]

[0057] It can be seen from Table 4 that under the above-mentioned culture conditions, the culture period of photosynthetic bacteria is 7 days, the concentration of the bacteria solution can reach more than 2 billion per milliliter, and the bacteria solution can be stor...

Embodiment 3

[0059] Outdoor open culture test

[0060] The preparation method of the photosynthetic bacteria culture medium is the same as in Example 1. Wash the 25L white drum. If it is a new drum, it can be used directly after cleaning. If it has been used, spray it with 20-30 times the dilution of Antiformin The inner wall is then washed with clear water, and 20% of the strains are connected to the barrel, and then 80% of the culture solution is added, and the lid is covered and placed in the outdoor sunlight for cultivation. The daily average temperature is above 25°C, and the weather is fine. Samples are taken every day to measure the concentration of the bacterial solution.

[0061] Table 5 The concentration of photosynthetic bacteria changes with time (10 8 pieces / ml)

[0062]

[0063] The test results show that in summer, photosynthetic bacteria can reach the maximum concentration of bacteria liquid in 5 consecutive sunny days or 7 intermittently accumulated sunny days, and th...

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Abstract

The invention provides a simple culture method of photosynthetic bacteria with a high cell concentration. The components of the culture medium of photosynthetic bacteria are simplified. The main components of the culture medium comprise the following steps: 0.3% of sodium acetate, 0.6% of sodium bicarbonate, 0.1% of NH4Cl, 0.1% of NaCl, 0.02% of MgSO4.7H2O, 0.05% of K2HPO4, 0.02% of KH2PO4, 0.005% of CaCl2 and 0.05% of yeast extract. The production process of photosynthetic bacteria is also simplified. The invention has the following advantages: the composition and components of the culture medium are optimized, and the production cost can be reduced under the premise of ensuring the concentration of a bacteria solution; the culture process of photosynthetic bacteria is simplified to ensure that the scale production of photosynthetic bacteria has high operability; and the required equipment is simple and culturists can produce photosynthetic bacteria at home so as to greatly reduce the use cost of photosynthetic bacteria. The photosynthetic bacteria produced by the method can be used to adjust the algal phase of a water body, effectively reduce the ammonia-nitrogen content and increase the content of dissolved oxygen in the water body.

Description

technical field [0001] The invention relates to the fields of biotechnology and microbial application, in particular to a simple culture method for photosynthetic bacteria with high cell concentration and the application of fermentation liquid to regulate water quality. [0002] Specifically, it is a simple and practical production method of photosynthetic bacteria with high cell concentration and the application of fermented liquid in regulating water quality in aquaculture. Background technique [0003] In recent years, with the continuous expansion of aquaculture breeding area and production scale, as well as the sharp increase of aquaculture production and the continuous improvement of aquaculture intensification, aquaculture production itself has produced a certain degree of pollution to the environment of aquaculture waters; in addition, human life Excessive discharge of sewage, various industrial wastewater, and agricultural backwater further pollutes water sources fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCY02W10/37
Inventor 谢凤行赵玉洁张峰峰周可
Owner TIANJIN CITY AGRI BIO TECH RES CENT
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