Processing method for activity stability of enzyme in human serum
A processing method, human serum technology, applied in the preparation of test samples, etc., can solve the problems of inconsistent and unstable measurement results, large variation of measurement results of the analysis system, etc., to meet the needs of clinical use and solve interoperability problems
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[0031] Step 1. Collect the fresh human serum on the day of the experiment and place it in a closed freeze-thaw tube, freeze it below -60°C until about 1000ml is collected.
[0032] Step 2. Take the serum out of the freezer and place it naturally at room temperature for 4 hours until it is completely dissolved by visual observation, and then freeze it below -60°C for more than 2 hours; then place the serum at 4°C for 10 hours until it is completely dissolved by naked eye observation , and then freeze at -60°C for 2 hours; then rinse the freeze-thaw tube containing the serum with water at 10°C until it is completely dissolved by visual inspection.
[0033] Step 3: Use low-high speed ∮ 15-30cm qualitative filter paper to filter out impurities such as fibrin filaments, various antigen-antibody complexes and protein complex particles in the serum.
[0034] Step 4. The human serum after primary filtration is aseptically filtered under conventional aseptic conditions, using a micropo...
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