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Chicken Flavivirus and inactivated vaccine thereof

A virus inactivation and flavivirus technology, which is applied in antiviral agents, virus/bacteriophage, virus antigen components, etc., can solve the problem of no preventive vaccine, etc., and achieve the effect of easy safety evaluation, convenient operation, and good antigenicity

Active Publication Date: 2013-04-03
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the disease is a new disease, there is currently no vaccine to prevent the disease at home and abroad

Method used

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  • Chicken Flavivirus and inactivated vaccine thereof
  • Chicken Flavivirus and inactivated vaccine thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Chicken yellow virus FQ-C1 strain of the present invention is obtained in the following manner:

[0013] In December 2010, a 210-day-old laying hen flock in a laying hen farm in Fuqing City experienced an acute and severe drop in egg production, but no chickens died, and the use of antibacterial and antiviral drugs had no obvious effect. We isolated a strain of virus from the tissues of dead diseased laying hens, and ruled out avian influenza virus, chicken egg drop syndrome virus, chicken Newcastle disease virus, chicken infectious bronchitis virus, chicken infectious laryngotracheitis through laboratory testing Viruses, chicken arthritis virus, chicken infectious bursal virus, through flavivirus-specific RT-PCR identification and sequencing analysis, it was shown that the FQ-C1 strain virus belongs to the Flavivirus genus of the family Flaviviridae.

[0014] (1) Treatment of diseased materials: Aseptically collect hemorrhage follicles from laying hens showing abnormal...

Embodiment 2

[0021] Chicken yellow virus inactivated vaccine of the present invention is obtained in the following manner:

[0022] Chicken yellow virus FQ-C1 strain is inoculated into SPF chicken embryo, collects dead SPF chicken embryo allantoic fluid after inoculation, wherein the content of chicken yellow virus FQ-C1 strain is 10 3.33 ELD 50 / 0.2ml, SPF chicken embryo allantoic fluid is inactivated by formaldehyde and added white oil adjuvant to emulsify, and is prepared into a conventional oil emulsion inactivated vaccine.

[0023] The immune result of the prepared inactivated vaccine is as follows:

[0024] 1. Vaccine safety test

[0025] 1.1 Safety test of SPF chicks

[0026] 60 1-day-old SPF chicks were purchased, and they were randomly divided into 4 groups when they were 7 days old, with 15 chickens in each group. The SPF chickens in the 3 experimental groups were injected intramuscularly with different batches (3 batches) of chicken yellow virus through the legs. The in...

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Abstract

The invention relates to a chicken yellow virus and an inactivated vaccine thereof, belonging to the field of veterinary epidemiology. Chicken Flavivirus of the present invention is Chicken Flavivirus (Chicken Flavivirus) FQ-C1 strain, which has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on May 30, 2011, and the preservation registration number is CGMCCNO.4905. Chicken yellow virus FQ-C1 strain was inoculated with SPF chicken embryos, and the allantoic fluid of SPF chicken embryos that died after inoculation was collected. The allantoic fluid of SPF chicken embryos was inactivated by formaldehyde and added with white oil adjuvant for emulsification to prepare a conventional oil emulsion. Inactivated vaccines. The inactivated chicken yellow virus vaccine prepared by the invention will not cause any adverse reaction to chickens, and has high experimental safety. The vaccine can effectively control chicken yellow virus disease, reduce losses and improve breeding benefits for a large number of chicken producers in my country.

Description

technical field [0001] The invention relates to a chicken yellow virus and an inactivated vaccine thereof, belonging to the field of veterinary epidemiology. Background technique [0002] Since August 2010, laying hens or (and) breeders in Fujian, Guangdong, Shandong and other places in my country have experienced a disease characterized by a rapid decline in feed intake and egg production rate and low mortality, commonly known as chicken egg production shock. Down syndrome, chicken yellow virus disease. The laying hens and breeders with high egg production rate (such as more than 85%) that are sick will drop rapidly from the egg production rate to 20% to 30% 4 to 5 days after the onset, and stop production 7 to 10 days after the onset of the disease. According to incomplete statistics, the economic loss caused by the disease to my country's chicken industry has been as high as 30 million yuan since its occurrence. Because the disease is a new disease, there is no vaccine t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/12A61P31/14C12R1/93
Inventor 黄瑜傅光华万春和施少华程龙飞陈红梅林芳林建生
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI