Method for assisted selection of wheat variety with stripe rust resistance and special PCR reagent used therein

A stripe rust and reagent technology, applied in the field of PCR reagents to assist in the breeding of stripe rust-resistant wheat varieties, can solve the problems of few reports on the application effect.

Inactive Publication Date: 2011-11-02
INST OF CROP SCI CHINESE ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although there are some relevant studies in my country, there are few reports on specific application effects

Method used

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  • Method for assisted selection of wheat variety with stripe rust resistance and special PCR reagent used therein
  • Method for assisted selection of wheat variety with stripe rust resistance and special PCR reagent used therein
  • Method for assisted selection of wheat variety with stripe rust resistance and special PCR reagent used therein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Example 1, the discovery of a new anti-stripe rust gene Yrcaas and the acquisition of its SSR marker in wheat material Zhou 8425B:

[0091] 1. Acquisition of phenotypes and their performance at the seedling stage

[0092] Use Zhou 8425B to cross with Avocet S to obtain F 1 groups, the F 1 F 2 group, F 2 The population is 586 individual plants. The physiological race No. 32 among the current popular races of stripe rust in my country was used to inoculate and identify the experimental materials. The results showed that Zhou 8425B showed high resistance (reactive IT=0;) to immunity (IT=0) at the seedling stage. AvocetS showed high sensitivity (IT=4) at the seedling stage, F 1 All groups showed high resistance (IT=0;), F 2 In the population, 439 plants were resistant and 147 plants were susceptible, indicating that Zhou 8425B carried a pair of dominant genes highly resistant to stripe rust. According to the identification results at the seedling stage, 10 extremely r...

Embodiment 2

[0104] Embodiment 2, the breeding process of Cunmai No. 7 wheat

[0105] Cunmai 7 is a semi-winter, medium-dwarf wheat variety bred by crossing Zhoumai 24 / Zhoumai 22. For its pedigree, see figure 2 . This variety exhibits high resistance to mixed strains of stripe rust at the seedling stage, and is not only highly resistant to stripe rust at the adult stage, but also highly resistant to leaf rust. It has participated in the 2010-2011 national Huanghuai slice pre-test.

[0106] The selection process is as follows:

[0107] 1. F 1 Procurement of substitute wheat

[0108] Take Zhoumai 24 (a variety bred by Zhoukou Academy of Agricultural Sciences, which was protected by variety rights in 2006 (CNA002834E), and passed the national examination in 2007. The characteristics and cultivation techniques of Zhoumai 24, Wang Shigang, Seed Industry Guide, No. 05 in 2010 Issue, page 29, the public can obtain it from the Crop Science Research Institute of the Chinese Academy of Agricult...

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Abstract

The invention discloses a method for screening a wheat variety with stripe rust resistance assisted by molecular markers and special primers used therein. Sequences of the primer pairs provided in the invention for assisted selection of a wheat variety with stripe rust resistance are described in a primer sequence table (Table 1). The method for assisted selection of a wheat variety with stripe rust resistance provided in the invention is carried out on the basis of conventional breeding and causes no interruption to the breeding process of a breeder except that in the generations of F2 and F6, after the selection of the breeder, candidate individual plants are subjected to molecular marker detection and only those individual plants containing two stripe rust resistant genes are reserved,thereby helping the breeder to more effectively select excellent strains containing a plurality of stripe rust resistant genes and enhancing endurance of stripe rust resistance of the strains. According to the invention, a novel stripe rust resistant gene Yrcaas and its special primers are discovered in the wheat variety Zhou 8425B; the gene is on wheat 1B chromosome and is located between SSR mark Xbarc8 and SSR mark Xgwm582, wherein the two marks can be used for assisted breeding of stripe rust resistant plants. The special primers of the stripe rust resistance genes in the invention are important to the breeding of stripe rust resistant wheat.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for assisting in the breeding of stripe rust-resistant wheat varieties and a special PCR reagent thereof. Background technique [0002] Wheat stripe rust is an airborne leaf disease caused by wheat stripe rust (Puccinia striiformis f.sp.tritici), which will affect plant development and grain filling, and seriously affect wheat yield and quality. There are 43 million hm in the world 2 prone areas, accounting for 19.4% of the total wheat planting area ( http: / / www.cimmyt.org / agricdb / fao / Default.aspx). my country is the largest wheat stripe rust endemic area in the world, with about 20 million ha 2 (Stubbs, 1988; Wan et al., 2004, 2007), especially in cool and high altitude areas. Since the 1950s, there have been eight stripe rust pandemics in my country. Among them, the four pandemics in 1950, 1964, 1990 and 2002 had the largest area and the most serious damage. The secon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11A01H1/02
Inventor 何中虎郑天存任妍郑继东郑继周夏先春
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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