Fish sperm preserving fluid and preserving method

A technology of fish sperm and preservation method, applied in the field of animal reproduction, can solve the problem that artificial reproduction technology is not solved, and achieve the effects of prolonging in vitro survival time, improving survival index and simple preparation

Inactive Publication Date: 2011-11-23
吴江市水产养殖有限公司
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AI Technical Summary

Problems solved by technology

Due to the scarcity of semen and other reasons, the artificial reproduction

Method used

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Embodiment Construction

[0024] The preferred embodiments of the present invention are described in detail below, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, so as to define the protection scope of the present invention more clearly.

[0025] In order to improve the survival rate of fish sperm and realize the artificial reproduction of fish in the subfamily of Scorpio, the fish sperm preservation solution uses NaCl, KCl, CaCl 2 , MgSO 4 ·7H 2 O, NaHCO 3 、V C and other ingredients, prepared with distilled water.

[0026] The fish sperm preservation solution in the present embodiment comprises the following components:

[0027] NaCl 2.320g

[0028] KCl 1.902g

[0029] CaCl 2 0.328g

[0030] MgSO 4 ·7H 2 O 0.233g

[0031] NaHCO 3 0.264g

[0032] V C 0.228g

[0033] Distilled water 500mL.

[0034] Its preparation method is as follow...

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Abstract

The invention discloses a fish sperm preserving fluid which is prepared from 2.120-2.420g of NaCl, 1.602-2.202g of KCL, 0.178-0.478g of CaCl2, 0.133-0.333g of MgSO4.7H2O, 0.114-0.414g of NaHCO3, 0.128-0.328g of VC and 500mL of distilled water. The fish sperm preserving method comprises the following steps of: (a) sperm collection: wiping and drying the genital pore region of a mature milter by using a towel, slightly pressing the abdomen of the mature milter, and collecting the extruded sperm by using a suction tube; and (b) sperm preservation: putting the collected sperm into a clean container, adding the sperm preserving fluid of which the volume is 10-20 times of the volume of the sperm, and preserving in low-temperature light-tight places.

Description

technical field [0001] The invention relates to a fish sperm preservation solution and a preservation method, belonging to the technical field of animal reproduction. Background technique [0002] my country is a country rich in aquatic species resources, and the rich and diverse aquatic species resources have played a very important role in the development of my country's aquaculture industry. The subfamily of scorpionidae is a small fish that lives in the middle and lower layers of water bodies. It is distributed in most of the rivers and their affiliated water bodies in the plains of my country. Although the artificial reproduction technology of many fishes has been broken through, the artificial reproduction of fishes in the subfamily of scorpionidae has not yet been realized. Due to the long-term overfishing of fishery resources and the pollution of the water environment, the fish resources of the subfamily of Scorpidae have seriously declined and are on the verge of e...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 王荣泉宣云峰徐海华闫磊沈勤华
Owner 吴江市水产养殖有限公司
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