Pseudoxanthomonasjaponensis and microorganism microbial inoculum as well as applications thereof

A microbial inoculum, the technology of Pseudomonas, which can be used in microorganism-based methods, microorganisms, biological water/sewage treatment, etc., and can solve problems such as low degradation ability

Inactive Publication Date: 2011-11-23
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to overcome the shortcomings of existing microbial strains with low degradation ability to petroleum hyd

Method used

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  • Pseudoxanthomonasjaponensis and microorganism microbial inoculum as well as applications thereof
  • Pseudoxanthomonasjaponensis and microorganism microbial inoculum as well as applications thereof
  • Pseudoxanthomonasjaponensis and microorganism microbial inoculum as well as applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Enrichment and isolation of petroleum hydrocarbon degrading bacterial strains

[0047] Weigh 5g of the oil-contaminated soil mixture and add it to a 250mL Erlenmeyer flask filled with 100mL enrichment medium, cultivate it at 28°C and 180r / min on a constant temperature shaker, and transfer 5mL of the culture medium to a fresh enrichment culture after 5 days base, cultured under the same conditions for 5 days, and continuously enriched and cultured for 25 days in this way, the culture solution was diluted serially and spread on solid PDA medium, and cultured at 28°C; after colonies grew on the plate, single colonies of different shapes were picked Separation and purification were carried out repeatedly by streak separation until a pure culture of a single strain was obtained, and the purified strain was preserved in the slant of beef extract peptone medium.

[0048] 2. Screening of strains with high degradation activity

[0049] Inoculate the above-mentioned purified ...

Embodiment 2

[0054] According to the experimental content and experimental methods recorded in the "Common Bacteria System Identification Manual" [4] and "Actinomycete Systematic Principles, Methods and Practice" [5], the strains with the preservation number CGMCC No. 4797 were screened. Bacteria identification.

[0055] 1. Morphological analysis

[0056] The preservation number is CGMCC No. 4797 bacterial strain carries out morphological analysis and observes under optical microscope, and the results are as follows: figure 1 and 2 shown.

[0057] The observation of the colony morphology was carried out on solid TSA medium. After the strains were streaked and inoculated, they were cultured at a constant temperature of 28°C for 24 hours to observe the colony morphology. The colony of the strain with the preservation number CGMCC No. 4797 is light yellow, round, smooth in texture and raised on the surface. After picking and culturing for 24 hours, the bacterial lawn was stained and obse...

Embodiment 3

[0071] Weigh 10g of petroleum-contaminated soil mixture (1.25g of petroleum content) and add it into 100mL of beef extract peptone medium (preservation number is CGMCC No. 4797). The number of bacteria is 0.5×10 10 1) in a 250mL Erlenmeyer flask, cultured at 28°C and 180r / min on a constant temperature shaker. After 3 days, the oil content in the oil-contaminated soil mixture was detected, and the result was 0.49 grams. The degradation rate of the strain to oil was about 60.8 %.

[0072]

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PUM

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Abstract

The invention provides a pseudoxanthomonasjaponensis with the collection number of CGMCC No.4797 and a microorganism microbial inoculum containing the same and also provides an application of the pseudoxanthomonasjaponensis or the microorganism microbial inoculum to the degradation of petroleum hydrocarbon, an application to biological remediation of the petroleum pollution, an application to biological remediation of soil polluted by the petroleum and an application to biological remediation of water polluted by the petroleum. The pseudoxanthomonasjaponensis with the collection number of CGMCC No.4797 and the microorganism microbial inoculum containing the same have very strong capability of degrading the petroleum hydrocarbon and can be widely applied to degradation of the petroleum hydrocarbon and remediation of the petroleum pollution, such as the remediation of the soil polluted by the petroleum, the remediation of the water polluted by the petroleum and the like.

Description

[0001] technical field [0002] The present invention relates to a kind of pseudoxanthomonas japonicum ( Pseudoxanthomonas japonensis ), and a species containing the Pseudomonas japonicum ( Pseudoxanthomonas japonensis ) microbial agents as active ingredients and their application; more precisely, it involves a kind of Pseudomonas japonicus ( Pseudoxanthomonas japonensis ), and the microbial agent containing this Pseudomonas japonicum, also relates to the application of said Pseudomonas japonicus or microbial agent in the degradation of petroleum hydrocarbons, the application in bioremediation of oil pollution, the application in bioremediation Application in oil-contaminated soil, and application in bioremediation of oil-contaminated water bodies. [0003] Background technique [0004] In today's world, petroleum has become one of the most important energy sources for human beings. A large amount of petroleum and its processed products enter the soil, causing petroleum p...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C02F3/34C12R1/01A62D101/20
Inventor 郭萍朱昌雄田云龙刘雪李峰叶婧
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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