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A microbial method for screening acephate residues in food

A technology of acephate and food, applied in the field of acephate residues, can solve the problems of no screening, high or low sensitivity and specificity, affecting the accuracy and precision of microorganisms, etc., to achieve simple and easy detection methods, Sensitive and precise results

Inactive Publication Date: 2011-12-07
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sensitivity and specificity of the working strains to be tested are the most critical issues affecting the accuracy and precision of microbiological methods
So far, there is no relevant report on the screening of working strains of bifidobacteria with high sensitivity and specificity for acephate and its application in the screening of acephate residues in food

Method used

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  • A microbial method for screening acephate residues in food
  • A microbial method for screening acephate residues in food
  • A microbial method for screening acephate residues in food

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1 Isolation and identification of Bifidobacterium longum LJM002 strain

[0039] Using the feces of a healthy young man in Zhejiang Province as an isolated sample, the Bifidobacterium longum LJM002 described in the present invention was obtained by spreading and culturing on the improved MRS agar medium under anaerobic conditions at 37°C for 48 hours, and was identified as longum bifidobacterium LJM002. Bifidobacterium, the bacterial strain has been preserved on May 25, 2011 in the General Microbiology Center of China Committee for the Collection of Microbial Cultures (Address: No. 3, Datun Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Zip Code 100101), The classification name is Bifidobacterium longum, and the preservation number is CGMCC No.4900.

[0040] The formula of the improved MRS agar medium: peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, sodium acetate 5g, K 2 HPO 4 2g, MgSO 4 ·7H 2 O 0.5g,...

Embodiment 2

[0057] Example 2 Screening of acephate residues in Chinese cabbage samples

[0058] 1. Preparation of screening medium:

[0059] Peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, sodium acetate 5g, K 2 HPO 4 2g, MgSO 4 ·7H 2 O 0.5g, MnSO 4 4H 2 O 0.2g, fructooligosaccharide 3g, diammonium citrate 2g, Tween-80 1ml, agar 15g, 5-bromo-4-chloro-3-indole-β-D galactoside (X-Gal) 0.1g , 1L of distilled water, heat and dissolve the above ingredients to correct the pH to 6.5, and autoclave at 115°C for 15-20 minutes.

[0060] 2. Bacterial suspension preparation:

[0061] Bifidobacterium longum LJM002 was inoculated on the slant medium of nutrient agar, cultured anaerobically at 35±2°C for 24 hours, washed the bacterial lawn with sterilized normal saline, made a suspension, and placed the light in a 721 spectrophotometer at a wavelength of 580nm The transmittance is controlled at 0.8, and stored in a refrigerator at 2-8°C.

[0062] 3. Preparation of bacteria layer ...

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Abstract

The invention provides a Bifidobacterium longum LJM002 strain, of which the collection number is CGMCC No.4900. The LJM002 strain is super-sensitive to acephate, and thus, is a working strain for detecting acephate residues in food. The invention also provides a microbiological method for detecting acephate residues in food, which determines whether the residue level of acephate in a food sample to be detected is overproof according to the size of the inhibition zone on the detection culture medium. The method provided by the invention is simple to operate, has the characteristics of low cost and high sensitivity, is suitable for sample detection, and satisfies the detection requirements of Chinese Pesticide Residue Detection Department for mass samples.

Description

technical field [0001] The invention relates to a bifidobacterium longum strain and its application as a working strain in screening acephate residues in food. Background technique [0002] Acephate, whose chemical name is O,S-dimethyl, N-acetylthiophosphoramide, is an N-acetyl derivative of the insecticide methmaidophos. The international common English name is Acephate, molecular formula C 4 h 10 NO 3 PS, molecular weight 183.16, structural formula: [0003] [0004] Acephate is a broad-spectrum organic phosphorus insecticide with high efficiency, long-lasting effect and strong systemic property. It has stomach poisoning and contact killing effects, can kill eggs, and has certain fumigation effect. Tea trees, rice, wheat and other crops can effectively control a variety of chewing and sucking mouthparts pests. [0005] However, the excessive use of acephate makes it highly residual in vegetables and fruits, which poses a huge threat to people's health, so it has be...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12R1/01
Inventor 刘佳明方芳王文蔚
Owner WENZHOU MEDICAL UNIV
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