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A method for fluorescent labeling of fish otoliths

A fluorescent labeling and labeling technology, applied in fish farming, application, climate change adaptation, etc., can solve problems such as waste, side effects of fish, and unavailability, to improve utilization, reduce potential pollution, and mark low-cost products. Effect

Inactive Publication Date: 2011-12-14
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the three pairs of otoliths, micro otoliths and sagittal otoliths are more sensitive to alizarin complexes, while aster otoliths are less sensitive
[0006] However, according to the physical properties, it can be seen that the alizarin complex is slightly soluble in water, and other co-solvents are required to achieve a markable concentration
The use of co-solvents has certain side effects on fish
Therefore, using the method of soaking and marking otoliths, only a very small amount of markers will precipitate on the otoliths, most of the markers may exist in water and cannot be used, and the utilization rate of markers is extremely low. Large amount, serious waste and other problems, and will cause certain adverse effects on the environment

Method used

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  • A method for fluorescent labeling of fish otoliths
  • A method for fluorescent labeling of fish otoliths
  • A method for fluorescent labeling of fish otoliths

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Select alizarin complex as a fluorescent marker, mix it with fish compound feed at a mass ratio of 1:10, add a small amount of water to moisten the feed and alizarin complex, and stir evenly to make the whole feed purple Red, dried, ground to make marked feed of appropriate particle size;

[0028] 2) Feed enough marked feed to feed the young Cantonese bream for more than 24 hours.

[0029] After the marking is over, take out the otoliths of the juvenile Cantonese bream, clean the otoliths with absolute ethanol, remove the impurities on the surface of the otoliths, and observe the otoliths under a fluorescent microscope, which can be seen as figure 1 with figure 2 otolith marking wheel. Among them: 1 is the image observed under the excitation of yellow-green light; 2 is the image observed under the excitation of blue light; 3 is the image observed under the excitation of purple light.

Embodiment 2

[0031] 1) Mix alizarin complex with fish feed at a ratio of 1:40 to make marked feed;

[0032] 2) Feed enough marked feed to feed the young Cantonese bream for more than 24 hours.

[0033] After marking, the otoliths of young Cantonese bream were taken out, and the otoliths were cleaned with absolute ethanol to remove impurities on the surface of the otoliths. The otoliths were observed under a fluorescence microscope, and it was found that clear marking wheels were formed on the micro otoliths. The result is as image 3 shown. Among them: 1 is the image observed under the excitation of yellow-green light; 2 is the image observed under the excitation of blue light; 3 is the image observed under the excitation of purple light.

[0034] tag feed

[0035] Mark feed, containing alizarin complex. Preferably, in order to ensure the normal feeding of fish, the alizarin complex accounts for about 2-10% of the feed. It can be obtained by uniformly mixing alizarin complex with co...

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PUM

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Abstract

The invention discloses a new method for fluorescent labeling of fish otoliths. The invention mixes the alizarin complex and the compound feed for fish to prepare the marked feed, and then feeds the fish to be marked, so that the identifiable fluorescent mark can be detected on the otolith of the marked fish. It avoids the disadvantages of other marking methods such as greater damage to the fish body, high mark loss rate, expensive mark, low retention rate, and limited number of marks. This method solves the waste and pollution problems of the alizarin complex indicator in the otolith fluorescent labeling method by soaking. Suitable for mass release marking.

Description

technical field [0001] The invention relates to a method for fluorescently marking fish otoliths. Background technique [0002] The otolith of fish is a kind of calcified tissue, which exists in the membranous labyrinth of the inner ear, and acts as a balance and auditory tissue. Teleost fish generally have three pairs of otoliths, sagittal otoliths, micro otoliths and star otoliths, which exist in the inner ear respectively. in the balloon, utricle and listening pot. During the process of material exchange between fish and the external environment, chemical elements in the environment are continuously ingested into the fish body, undergo a series of metabolism and circulation in the fish body, and finally deposit on the otoliths. Therefore, by changing the environment in which fish grow, fish can form specific marks on otoliths to distinguish them from natural populations. [0003] The mark-recapture method is usually used to evaluate the effect of fish proliferation and ...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 李跃飞李新辉王超杨计平谭细畅李捷赖子尼吴茜
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI