Application of SB-3CT (2-[(4-phenoxyphenyl) sulfonylmethyl] thiirane) in treating cerebrovascular spasm (CVS)
A cerebral vasospasm and drug technology, applied in the field of new drug use research, can solve problems such as cerebral vasospasm that have not been studied, and achieve the effects of reducing the degree of spastic blood vessel wall thickening and the degree of lumen stenosis.
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[0033] 1. Materials and methods
[0034] 1. Materials
[0035] 1.1 Reagents and experimental equipment
[0036] 1.1.1 Main Drugs and Reagents
[0037] SB-3CT, purchased from Sigma, USA; paraformaldehyde, PBS, DAB chromogen, hematoxylin, eosin, glacial acetic acid (analytical grade), normal saline, distilled water, 10% chloral hydrate. Rabbit Anti-MMP-9 was purchased from Beijing Boaosen Biotechnology Co., Ltd., China.
[0038] 1.1.2 Main instruments
[0039] Paraffin slicer (Leica 3215, Germany), automatic dehydrator (Leica 1020, Germany), paraffin embedding machine (BMJ-III, Changzhou, China), Olympus BX40 optical microscope, image photography software (LOGENE-I Path PRT diagnostic report Workstation), a set of surgical instruments, etc.
[0040] 1.2 Experimental animals
[0041] Adult healthy male SD rats were provided by the Experimental Animal Research Center of Soochow University School of Medicine (Su SYXK2002-2010, clean level: level 2), weighing 250-300 g. Befor...
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