Tissue culture and rapid propagation method of chimera hosta plants

A technology of tissue culture rapid propagation and chimera, which is applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of not meeting the needs of production and market, low reproduction coefficient, long cycle, etc.

Inactive Publication Date: 2011-12-21
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, hosta is mostly propagated by ramification, which has a low reproduction coefficient and a long cycle, which cannot meet the needs of production and the market.

Method used

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  • Tissue culture and rapid propagation method of chimera hosta plants
  • Tissue culture and rapid propagation method of chimera hosta plants
  • Tissue culture and rapid propagation method of chimera hosta plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Test material: Take healthy, disease-free, 2-3 cm long green-edged yellow-core chimera sprouts as explants.

[0022] (2) Obtaining sterile materials: Soak in washing powder water for about 10-15 minutes and gently wash with soft hair, then rinse with running water for 30-45 minutes, soak in 75% alcohol for 30-45 seconds, and use 0.1% mercury liter Soak for 10-15min, stir continuously, rinse with sterile water for 3-5 times, blot dry with filter paper, cut into 0.5-1cm 3 The square of the shoot, the base of the young shoot is inserted into the culture medium for observation.

[0023] (3) Medium: It is easier to induce clustered buds in the medium supplemented with 6-BA3.0mg / L and NAA0.5mg / L in MS; the combination of 6-BA2.0mg / L and NAA0.3mg / L is the most effective The medium suitable for bud proliferation; 1 / 2MS as the basic medium, adding 0.3mg / LNAA and 1.0mg / LIBA and 0.2% activated carbon is most conducive to the rooting of hosta test tube seedlings. At the same ...

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Abstract

The invention provides a method for tissue culture and rapid propagation of chimera hosta plants, which includes disinfection, primary culture, subculture and rooting culture, and utilizes young buds of chimera hosta as explants for tissue culture and rapid propagation. The method has a high reproduction coefficient, is suitable for commercial production, and fills a gap in market demand for chimera hostas. The present invention uses the young shoots of the chimera hosta as explants, and studies the plant regeneration and rapid propagation technology of the hosta flower. The whole industrialization process of plant regeneration, multiplication, rooting and seedling formation is completed by the invention, and the high proliferation coefficient of the culture medium can meet the large demand of commercial production.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of chimera hosta plants. Background technique [0002] Chimera is the same individual or organ formed by the co-development of cells with different genetic traits, and cell mutation is the basis for the formation of chimera. Mutations can occur in any type of plant. Plant chimera refers to plant tissues or plant individuals containing two or more genotypes. Baur (1909) conducted a detailed study on the yellow-edged green-core geranium (Pelargonium zonale) plant and found that the shoot apical meristem (SAM), stem, petiole and leaves were composed of two genetic types of cells. Formed regularly, it is called plant chimera. On this basis, the basic theory of plant chimera has been formed after further research, which provides a basis for the application of plant chimera and the breeding of exotic flowers and plants. [0003] Hosta ( Hosta plantiginea Ascherson), also known a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李房英吴少华何碧珠关夏玉钟凤林
Owner FUJIAN AGRI & FORESTRY UNIV
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