Multi-strain composition for efficiently degrading environmental organophosphorus pesticide residues

A technology of organophosphorus pesticides and compositions, applied in the field of environmental bioengineering, can solve the problems of slow degradation of bacterial strains, lack of rapid degradation, and difficulty in rapid removal, etc., and achieve the effect of removing pollution

Active Publication Date: 2011-12-28
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

①. There are differences in the concentration of organophosphorus pesticide residues in different environments, and there are also differences in the degradation ability of strains screened by different laboratories. Some strains degrade organophosphorus residues at a higher concentration of 50 mg / L to 100 mg / L faster, and it is not easy to quickly remove organophosphorus pesticide residues in the environment with the preparation of such a single strain
②. There is a lack of high-efficiency degrading strains that can rapidly degrade organophosphorus pesticide residues higher than 100 mg / L in the existing technology
③. Generally, the degradation rate of the strains used is slow, and it takes 7 days to more than 30 days for the treatment time to fully exert the degradation effect

Method used

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  • Multi-strain composition for efficiently degrading environmental organophosphorus pesticide residues
  • Multi-strain composition for efficiently degrading environmental organophosphorus pesticide residues
  • Multi-strain composition for efficiently degrading environmental organophosphorus pesticide residues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Degradation of multi-strain composition to 100mg / L high concentration pesticide chlorpyrifos in water sample

[0027] 1. Bacteria solution preparation

[0028] Prepare A bacteria solution, B bacteria solution and C bacteria solution respectively:

[0029] (1), strain A is Rhodococcus rhodococcus ( Rhodococcus rhodochrous ) R-D3 strain,

[0030] (2), B strain is Microacidophilus Stenotrophomonas ( Stenotrophomonas acidaminiphila ) G1 strain,

[0031] (3), C strain is Copperophilus Taiwan ( Cupriavidus taiwanensis ) X1 strain,

[0032] Prepare LB liquid culture medium, aseptically inoculate a ring of bacteria from the slant of A strain, B strain and C strain into LB liquid medium; place the inoculated medium at constant temperature Cultivate in a shaking incubator, the culture conditions are: 30°C, 120 rpm, and culture for 24 hours; the cell concentration is 5.0×10 9 / mL of A bacteria solution, cell concentration 8.0×10 9 / mL of B bacteria solution an...

Embodiment 2

[0050] Example 2: Degradation of 50mg / L high concentration pesticide chlorpyrifos in water sample by suspension cell multi-strain composition

[0051] 1. Bacteria solution preparation

[0052] Prepare A bacteria solution, B bacteria solution and C bacteria solution respectively:

[0053] Aseptically inoculate a loop of bacteria from the slant of strain A, strain B and strain C into LB liquid medium; culture the inoculated medium in a constant temperature shaking incubator, The culture conditions are: 30°C, 120 rpm, and culture for 30 hours; the cell concentration is 6.0×10 9 / mL of A bacteria solution, cell concentration 9.0×10 9 / mL of B bacteria solution and cell concentration 7.0×10 9 / mL of C bacteria solution, the temperature is 4 ℃ for later use.

[0054] Obtain the cells in the bacterial liquid by centrifugation, centrifuge at a speed of 5000 rpm, and centrifuge for 20 minutes; discard the supernatant, and suspend the centrifuged precipitated cells with 100 mL of 0...

Embodiment 3

[0069] Example 3: Degradation of 20mg / L low-concentration pesticide chlorpyrifos in soil by solid multi-strain composition

[0070] 1. Bacteria solution preparation

[0071] The preparation of A bacteria solution, B bacteria solution and C bacteria solution is the same as above, and the cell concentration is 5.0×10 respectively. 9 / mL of A bacteria solution, cell concentration 8.0×10 9 / mL of B bacteria solution and cell concentration 6.0×10 9 / mL of C bacteria solution.

[0072] 2. Preparation of solid-state multi-strain combination bacteria

[0073] Take 100mL of A bacterial solution, 50mL of B bacterial solution and 50mL of C bacterial solution, mix them evenly according to the volume ratio of 2:1:1, add 250g of solid adsorbent peat, and mix well to obtain a solid multi-strain composition; temperature Store at 4°C for later use.

[0074] 3. Degradation of pesticide chlorpyrifos in soil by multi-strain composition

[0075] Take 500g of air-dried soil and put it in a p...

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Abstract

The invention relates to a multi-strain composition for high-efficiency degradation of organic phosphorus pesticides in the environment, which consists of a strain A, a strain B and a strain C, wherein the strain A is rhodococcus rhodochrous R-D3 strain; the B strain is stenotrophomonas acidaminiphila G1; and the C strain is cupriavidus taiwanensis X1 strain. The strain A, strain B and strain C are inoculated onto and cultured in a Luria Broth (LB) liquid culture medium respectively to form bacterial solution A, bacterial solution B and bacterial solution C; the bacterial solution A, bacterial solution B and bacterial solution C are uniformly mixed according to a volume mixing ratio of (1-2):(1-2):(1-2); and thus, the multi-strain composition for high-efficiency degradation of organic phosphorus pesticides in the environment is obtained. The multi-strain composition can quickly degrade organic phosphorus pesticide residue to different degrees within 1 to 7 days, the degradation rate of the multi-strain composition reaches over 95 percent, and the pollution of organic phosphorus pesticides is eliminated effectively.

Description

technical field [0001] The invention belongs to environmental bioengineering, in particular to a mixed microbial agent for efficiently degrading residual organophosphorus pesticides in the environment. Background technique [0002] my country is a large agricultural country, and the production and usage of pesticides are increasing year by year. The extensive application of pesticides in agricultural production has played an extremely important role in the prevention and control of crop diseases and insect pests, providing a reliable guarantee for a bumper harvest. However, the long-term and large-scale use of pesticides has made the pesticide pollution in the environment more and more serious, endangering people's health, food safety and other environmental problems. Organophosphorus pesticides have been widely and widely used for a long time, among which chlorpyrifos is one of the most used pesticides in the world. In my country, chlorpyrifos is a new type of high-effici...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/04A62D101/28
Inventor 王道胜唐欣昀花日茂朱贝贝高婷婷吴祥为
Owner ANHUI AGRICULTURAL UNIVERSITY
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