Multi-strain composition for efficiently degrading environmental organophosphorus pesticide residues

A technology of organophosphorus pesticides and compositions, applied in the field of environmental bioengineering, can solve the problems of slow degradation of bacterial strains, lack of rapid degradation, and difficulty in rapid removal, etc., and achieve the effect of removing pollution

Active Publication Date: 2011-12-28
ANHUI AGRICULTURAL UNIVERSITY
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

①. There are differences in the concentration of organophosphorus pesticide residues in different environments, and there are also differences in the degradation ability of strains screened by different laboratories. Some strains degrade organophosphorus residues at a higher concentration of 50 mg/L to 100 mg/L faster, and it is not easy to quickly remove organophosphorus pesticide residue

Method used

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  • Multi-strain composition for efficiently degrading environmental organophosphorus pesticide residues
  • Multi-strain composition for efficiently degrading environmental organophosphorus pesticide residues
  • Multi-strain composition for efficiently degrading environmental organophosphorus pesticide residues

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0026] Example 1: Degradation of 100mg / L high-concentration pesticide chlorpyrifos in water samples by multi-strain composition

[0027] 1. Bacteria preparation

[0028] Prepare bacteria A, B and C respectively:

[0029] (1) Strain A is Rhodococcus rosea ( Rhodococcus rhodochrous ) R-D3 strain,

[0030] (2) Strain B is Stenotrophomonas acidophilus ( Stenotrophomonas acidaminiphila ) G1 strain,

[0031] (3) Strain C is Taiwan Copperophilus ( Cupriavidus taiwanensis ) X1 strain,

[0032] Prepare LB liquid medium. Take a loop of strains from the slant surface of strain A, the slant surface of strain B, and the slant surface of strain C into LB liquid medium aseptically; place the inoculated medium at a constant temperature Cultivate in a shaking incubator, the culture conditions are: 30℃, 120 revolutions / min, 24 hours of culture; the cell concentration is 5.0×10 9 / mL of bacteria A, cell concentration 8.0×10 9 / mL B bacteria solution and cell concentration 6.0×10 9 / mL of C bacteria liqu...

Example Embodiment

[0050] Example 2: The degradation of 50 mg / L high-concentration pesticide chlorpyrifos in water samples by suspension cell multi-strain composition

[0051] 1. Bacteria preparation

[0052] Prepare bacteria A, B and C respectively:

[0053] Take a loop of strains from the slope of strain A, the slope of strain B and the slope of strain C aseptically to inoculate the LB liquid medium; place the inoculated medium in a constant temperature shaking incubator for cultivation, The culture conditions are: 30°C, 120 revolutions / min, culture for 30 hours; the cell concentration is 6.0×10 9 / mL of A bacteria liquid, cell concentration 9.0×10 9 / mL B bacteria liquid and cell concentration 7.0×10 9 / mL of C bacteria liquid at 4℃ for use.

[0054] Take the centrifugal method to obtain the cells in the bacterial solution, the centrifuge speed is 5000 rpm, centrifugation for 20 minutes; discard the supernatant, use 100mL of 0.85% sodium chloride solution to suspend the centrifuged cells, respective...

Example Embodiment

[0069] Example 3: Degradation of 20mg / L low-concentration pesticide chlorpyrifos in soil by solid multi-strain composition

[0070] 1. Bacteria preparation

[0071] The preparation of A bacteria liquid, B bacteria liquid and C bacteria liquid is the same as above, and the cell concentration is 5.0×10 9 / mL of bacteria A, cell concentration 8.0×10 9 / mL B bacteria solution and cell concentration 6.0×10 9 / mL of C bacteria liquid.

[0072] 2. Preparation of solid multi-strain combination bacteria

[0073] Take 100mL of A bacteria liquid, 50mL of B bacteria liquid and 50mL of C bacteria liquid, and mix, that is, mix evenly according to a volume ratio of 2:1:1, add 250g of solid adsorbent peat, and mix well to obtain a solid multi-strain composition; 4℃ for use.

[0074] 3. Degradation of pesticide chlorpyrifos in soil by multi-strain composition

[0075] Take 500g of air-dried soil and place it in a plastic pot, take 200mL of distilled water, add 1mL of pesticide chlorpyrifos at a concent...

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Abstract

The invention relates to a multi-strain composition for high-efficiency degradation of organic phosphorus pesticides in the environment, which consists of a strain A, a strain B and a strain C, wherein the strain A is rhodococcus rhodochrous R-D3 strain; the B strain is stenotrophomonas acidaminiphila G1; and the C strain is cupriavidus taiwanensis X1 strain. The strain A, strain B and strain C are inoculated onto and cultured in a Luria Broth (LB) liquid culture medium respectively to form bacterial solution A, bacterial solution B and bacterial solution C; the bacterial solution A, bacterial solution B and bacterial solution C are uniformly mixed according to a volume mixing ratio of (1-2):(1-2):(1-2); and thus, the multi-strain composition for high-efficiency degradation of organic phosphorus pesticides in the environment is obtained. The multi-strain composition can quickly degrade organic phosphorus pesticide residue to different degrees within 1 to 7 days, the degradation rate of the multi-strain composition reaches over 95 percent, and the pollution of organic phosphorus pesticides is eliminated effectively.

Description

technical field [0001] The invention belongs to environmental bioengineering, in particular to a mixed microbial agent for efficiently degrading residual organophosphorus pesticides in the environment. Background technique [0002] my country is a large agricultural country, and the production and usage of pesticides are increasing year by year. The extensive application of pesticides in agricultural production has played an extremely important role in the prevention and control of crop diseases and insect pests, providing a reliable guarantee for a bumper harvest. However, the long-term and large-scale use of pesticides has made the pesticide pollution in the environment more and more serious, endangering people's health, food safety and other environmental problems. Organophosphorus pesticides have been widely and widely used for a long time, among which chlorpyrifos is one of the most used pesticides in the world. In my country, chlorpyrifos is a new type of high-effici...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/04A62D101/28
Inventor 王道胜唐欣昀花日茂朱贝贝高婷婷吴祥为
Owner ANHUI AGRICULTURAL UNIVERSITY
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