Systems and methods for constructing sequencing libraries

A technology for sequencing libraries and purposes, applied in the field of genetic engineering, can solve problems such as self-ligation of joints, and achieve the effect of quick and easy establishment

Active Publication Date: 2011-12-28
WUHAN CONSIDERIN GENE & HEALTH TECH CO LTD
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  • Abstract
  • Description
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Problems solved by technology

[0005] One of the objects of the present invention is to provide a joint element, aiming to so

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  • Systems and methods for constructing sequencing libraries
  • Systems and methods for constructing sequencing libraries
  • Systems and methods for constructing sequencing libraries

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Embodiment Construction

[0053] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0054] In order to solve the problem of joint self-connection in the process of building the library in the prior art, the structure of the joint element provided by the present invention is:

[0055] From the 5' to the 3' end, a bifurcation-type double-stranded nucleic acid adapter comprising a bifurcation region and a pairing region in sequence, the base sequence between the double strands of the bifurcation region cannot be complementary paired, and each of the double strands of the bifurcation region Contains at least one primer binding site; the pairing region contains at least one restriction recognition site.

[0056] The bifurcation design of the above-mentioned bifurcation type double-stranded nucleic acid adapter can avoid the occurren...

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Abstract

The invention relates to the field of gene engineering, and provides a system and a method for constructing a sequencing library. The system and the method comprise a joint element and a method for applying the joint element to the construction of a sequencing library. The joint element is a bifurcate double-chain nucleic acid joint with a bifurcate region and a paired region orderly included at the 5' and the 3' ends, wherein two single chains of the bifurcate region respectively comprise at least one amplification primer binding site, and the paired region comprises at least one restrictionenzyme site. The joint element of the invention can prevent the self-connection phenomenon of the joint element and the occurrence of a non-object joint element-DNA fragment compound during the library construction.

Description

technical field [0001] The invention relates to the field of genetic engineering, more specifically, to a linker element and a method for constructing a sequencing library. Background technique [0002] At this stage, DNA fragments used for high-throughput gene sequencing are first prepared into sequencing libraries. The preparation of the sequencing library includes the following steps. First, the sample genome is randomly cut to obtain a large number of DNA fragments, and then the adapters are connected for amplification reaction. In the process of building the library, the biggest problem is that the self-ligation of the adapter is easy to occur when the adapter is connected. At the same time, due to the randomness of the adapter connection, it is easy to appear the non-target adapter element-DNA fragment complex that is connected to the same adapter at both ends of the DNA fragment. The waste of raw materials increases the complexity of product separation and purificati...

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Application Information

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IPC IPC(8): C12N15/11C12N15/10C12Q1/68C40B50/06
Inventor 盛司潼
Owner WUHAN CONSIDERIN GENE & HEALTH TECH CO LTD
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