Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of Pin1 Family Genes of Auxin Export Carrier in Maize and Sorghum Breeding

A technology for exporting vectors and auxin, which is applied in the field of bioengineering breeding of crops, and can solve problems such as abnormal growth and development of plants

Inactive Publication Date: 2011-12-28
SHANDONG UNIV
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Affecting the distribution and polar orientation of PIN1 protein leads to abnormal plant growth and development

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Transformation of Prsp::ZmPIN1a gene to create drought-tolerant and low-phosphorus inbred lines of maize and its application

[0052] Construction of fusion gene and plant expression vector

[0053] In order to realize the specific expression of the transgenic ZmPIN1a in maize roots, it needs to be connected with a root-specific expression promoter to construct a fusion gene. The barley phosphorus transporter 1 gene is specifically expressed in roots and induced by low phosphorus stress (Schünmann et al., 2004). The promoter part (843 bp upstream of the ATG codon 5' in GenBank accession number AF543197, incomplete long) was amplified from the barley genome and named Prsp. The promoter was recombined into the intermediate vector after sequencing. Then utilize the Hind III and Xbal I restriction sites to recombine the promoter directionally into the plant expression vector with the glyphosate resistance gene EPSP as the plant selection marker to obtain the pl...

Embodiment 2

[0084] Example 2: Transformation of Prsp::ZmPIN1b gene to create drought-tolerant and low-phosphorus inbred lines of maize and its application

[0085] Construction of fusion gene and plant expression vector

[0086] In order to realize the specific expression of the transgenic ZmPIN1b gene in maize roots, the ZmPIN1b gene needs to be connected with the root-specific expression promoter Prsp (barley phosphorus transporter 1 gene promoter) to construct a fusion gene. The ZmPIN1b gene with positive sequencing was recombined into pCAMBIA-Prsp::MCS-Tnos-P35S::bar(pRPB) for Agrobacterium-mediated genetic transformation of maize. The vector contains the herbicide glufosinate-resistant bar gene initiated by P35S and the target gene initiated by Prsp. ZmPIN1b can be inserted into the multi-cloning site MCS in sense or antisense form to obtain the required plasmid. After the latter is identified by enzyme digestion or sequencing, it is introduced into Agrobacterium LBA4404 or other ba...

Embodiment 3

[0103] Example 3: Transformation of Prsp::ZmPIN1a or Prsp::ZmPIN1c gene to create sorghum lodging-resistant and low-phosphorus-tolerant materials

[0104] Construction of fusion gene and plant expression vector

[0105] The positive ZmPIN1a and ZmPIN1c were recombined into pCAMBIA-Prsp::MCS-Tnos-P35S::bar(pRPB), respectively. The vector contains the herbicide glufosinate resistance gene bar initiated by P35S and the target gene cassette initiated by Prsp. In the constructed plant expression vector, ZmPIN1a or ZmPIN1c can be respectively inserted into the multiple cloning site MCS in positive sense form to obtain the desired plasmid. After the latter is identified by enzyme digestion or sequencing, it is introduced into Agrobacterium LBA4404 or other strains by liquid nitrogen quick-freezing method, and used for Agrobacterium-mediated genetic transformation of sorghum.

[0106] Sorghum sterile vaccine obtained

[0107] The fine genotype seeds of sorghum are soaked in 70% eth...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses the use of auxin efflux carrier PINFORMED1 (PIN1) family genes in breeding corn and broomcorn. In the invention, different PIN1 family genes are cloned from different higher plants and bonded with different promoters respectively to form fusion genes; and the recombinant genes are transferred into corn and broomcorn cells by transgenic technology; and thus, transgenic plants and progeny of the transgenic plants are obtained, engineering plants of which the plant traits are changed with the gradient change in the concentration of auxin can be selected, and new varieties with application prospects in corn and broomcorn breeding can be created.

Description

technical field [0001] The invention belongs to the field of bioengineering breeding of crops, in particular, relates to a scheme and application for changing the traits of corn and sorghum by constructing fusion genes and transgenes, especially relates to the application of auxin export carrier PIN1 family genes in corn and sorghum breeding application. Background technique [0002] The development of plant genetic engineering depends on the application of genes with different functions. Auxin is synthesized in young parts of plants or vigorously dividing tissues, and reaches the site of action through its unique transport mode. Auxin plays an important role in plant growth and development. It regulates auxin synthesis, transport, metabolism and related signal transduction. The expression of guide genes may have an important impact on plant growth and development, and some of these genes related to auxin may be ideal targets for plant genetic engineering operations. [00...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/84A01H5/00
Inventor 张举仁李朝霞张新蕊
Owner SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com