Symbiosis composition and its preparation method

A composition and mixture technology, applied in food preparation, food science, animal feed, etc., can solve the problem of unsatisfactory symbiotic composition and achieve good homogeneity

Inactive Publication Date: 2011-12-28
ROSEBUD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] However, the symbiotic compositions propos

Method used

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  • Symbiosis composition and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0151] Example 1 - Preparation of Composition

[0152] The compositions of the present invention are prepared from the following compounds:

[0153] - cane molasses in crude condition from the Gardel Sugar Factory (Guadeloupe) especially for the type of food prepared in question;

[0154] - Dehydrated and encapsulated live microorganisms: Bifidobacterium longum type bacteria, sold by Lallemand (France) under the name Probiocap Rosell-175ME; the microbiological characteristics of this compound provided by Lallemand are as follows: Bifidobacterium longum > 5 × 10 10 cfu / g; Staphylococci 3 cfu / g; saprophytes 4 cfu / g; Pseudomonas 4 cfu / g; Salmonellae <1 cfu / 25g; Escherichia coli <1 cfu / g.

[0155] - Polysaccharide fiber: Nutriose (Roquette, France).

[0156] Microbiological characterization of molasses was performed prior to its use. For this, dilute molasses to 1 / 10 in LT100 broth and inoculate:

[0157] -To count the total aerobic bacteria, inoculate on Trypticase Soybea...

Embodiment 2

[0167] Example 2 - Effect of Compositions on Cell Viability

[0168] The combination obtained in Example 1 was evaluated from the viewpoint of viability of human colorectal cancer cells (Caco-2 line, clone TC-7) cultured in monolayer by the reduced MTT method (brominated thiazolyl blue tetrazolium) things. In DMEM supplemented with penicillin (Sigma, 50IU / mL), streptomycin (Sigma, 50mg / mL), gentamicin (Sigma, 10mg / mL) and fetal bovine serum (Sigma, 10%, v / v) Cell monolayers were grown to confluence in Acane MEM medium.

[0169] Dilute the final composition to 1 μg / mL, 10 μg / mL, 100 μg / mL, 1 mg / mL, 10 mg / mL, and 100 mg / mL, and place the cells at 37°C, containing 5% CO 2 Stored in a humid atmosphere for 48 days. EGF (epidermal growth factor) was used as a reference for the TC-7 cell proliferation assay.

[0170] Cell viability was measured according to the MTT reduction method. The reduction of MTT to Formazan Blue was assessed by measuring the absorbance, which is direct...

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Abstract

The present invention relates to a composition comprising: dry molasses in powder form, and dehydrated and encapsulated live microorganisms. The invention also relates to a food or dietary product or a medicament comprising said composition, and a method for preparing said composition comprising mixing said molasses and said dehydrated and encapsulated live microorganisms, and a drying step.

Description

technical field [0001] The present invention relates to symbiotic compositions (combinations of prebiotics and probiotics) and methods for their preparation. Background technique [0002] The beneficial effects of ingestion of certain live microorganisms on human or animal health have long been known. According to the Food and Agriculture Organization of the United Nations, these live microorganisms (designated "probiotics") provide health benefits to the host when administered in appropriate amounts. [0003] These probiotics (such as bifidobacteria, etc.) are used to make health food or pharmaceutical compositions. Certain compositions comprise encapsulated probiotics and food supplements (vitamins C and E, zinc proteinate, manganese proteinate, ferrous sulfate, copper proteinate, calcium iodide, and ferrous sodium selenate) (document WO 2007 / 126990). In certain compositions, the food supplement prolongs the life of the probiotics. [0004] It is generally believed tha...

Claims

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Application Information

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IPC IPC(8): A23K1/02A23K1/00A23L1/30A23L1/308A23K10/33
CPCA23L1/3014A23L1/308A23K1/009A23K1/02A23L1/30A23L1/09A23K10/18A23K10/33A23L29/30A23L33/10A23L33/135A23L33/21A23L33/125A23L7/00A23L5/00Y02P60/87
Inventor 布里吉特·阿莱曼德弗洛伦斯·普塞
Owner ROSEBUD
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