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Method for producing ethanol fuel from kitchen waste

A technology for kitchen waste and fuel ethanol, applied in the biological field, can solve the problems of easy leakage, high moisture content of garbage, and environmental pollution caused by urban daily traffic.

Active Publication Date: 2013-04-10
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, food waste has been used as feed for pigs in my country, and they have found their way out through the market. There are many problems in this treatment method: 1. The waste has high moisture content, high fluidity, and is easy to leak, causing secondary pollution
2. Garbage trucks affect daily urban traffic and pollute the environment
3. The garbage has not been treated in any way, and the sanitation of the pigs is worrying, which may easily lead to the occurrence of diseases such as foot-and-mouth disease
Chinese patent 200710017960.9 announced "a method for resource utilization of kitchen waste", which has already proposed a good treatment method, but there are defects in pollution discharge, etc., while Chinese patent ZL03117223.7 invented a method that uses rich fiber The method of producing fuel ethanol from organic waste of cellulose and hemicellulose requires acid resistance and pressure resistance, which requires high equipment and poor operability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Add biological bacteria liquid and enzyme preparations to 4 kg of food waste. Biological bacteria liquid, which contains the following ingredients: Bacillus licheniformis, Bacillus megaterium, Bacillus natto, Bacillus subtilis, Lactobacillus plantarum and Lactobacillus delbrueckii subsp. Bulgaria, Saccharomyces cerevisiae and Pichia fermentative bacteria liquid, which is added The mass ratio of each is 0.25%. Among them, the cell concentration of Bacillus licheniformis, Bacillus megaterium, Bacillus natto, Bacillus subtilis, Lactobacillus plantarum and Lactobacillus delbrueckii subsp. Bulgaria is 7×10 8 Pcs / ml to 8×10 8 Pcs / ml; the cell concentration of Saccharomyces cerevisiae and Pichia fermenting bacteria liquid is 6×10 8 Pcs / ml to 7×10 8 Pcs / ml. Add a mixed enzyme preparation with a mass ratio of 0.4%. The composition of the enzyme preparation is: amylase (200000u / g), cellulase (200000u / g), lipase (100000u / g), protease (100000u / g), fat Enzyme (100000u / g), pectinase ...

Embodiment 2

[0028] Add biological bacteria liquid and enzyme preparations to 4 kg of food waste. Biological bacteria liquid, which contains the following ingredients: Bacillus licheniformis, Bacillus megaterium, Bacillus natto, Bacillus subtilis, Lactobacillus plantarum, Lactobacillus delbrueckii subsp. Bulgaria, Saccharomyces cerevisiae and Pichia fermentans, and their addition ratios 0.4% each, among them, the cell concentration of Bacillus licheniformis, Bacillus megaterium, Bacillus natto, Bacillus subtilis, Lactobacillus plantarum and Lactobacillus delbrueckii subsp. Bulgaria is 7×10 8 Pcs / ml to 8×10 8 Pcs / ml; the cell concentration of Saccharomyces cerevisiae and Pichia fermenting bacteria liquid is 6×10 8 Pcs / ml to 7×10 8 Pcs / ml.

[0029] Add a mixed enzyme preparation with a mass ratio of 0.6%. The composition of the enzyme preparation is: amylase (200000u / g), cellulase (200000u / g), lipase (100000u / g), protease (100000u / g), fat Enzyme (100000u / g), pectinase (100000u / g), glucoamylase ...

Embodiment 3

[0032] Add biological bacteria liquid and enzyme preparations to 4 kg of food waste. Biological bacterial liquid, which contains the following ingredients: Bacillus licheniformis, Bacillus megaterium, Bacillus natto, Bacillus subtilis, Lactobacillus plantarum and Lactobacillus delbrueckii subsp. Bulgaria, Saccharomyces cerevisiae and Pichia fermentans, and their addition ratios 0.5% each, among them, the cell concentration of Bacillus licheniformis, Bacillus megaterium, Bacillus natto, Bacillus subtilis, Lactobacillus plantarum and Lactobacillus delbrueckii subsp. Bulgaria is 7×10 8 Pcs / ml to 8×10 8 Pcs / ml; the cell concentration of Saccharomyces cerevisiae and Pichia fermenting bacteria liquid is 6×10 8 Pcs / ml to 7×10 8 Pcs / ml. . A mixed enzyme preparation with a mass ratio of 0.6% is added, and the composition mass ratio of the enzyme preparation is amylase: cellulase: lipase: protease: pectinase: saccharification enzyme of 0.8:1.0:0.8:1.0:0.8:0.6. Then put the feed liquid w...

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PUM

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Abstract

The invention discloses a method for producing ethanol fuel from kitchen waste, belonging to the technical field of biology. The invention aims to reduce the environmental pollution of kitchen waste, and change wastes into valuable substances, thereby recycling the kitchen waste. According to the method, the kitchen waste, which contains rich starch, cellulose, fat, protein and the like, is comprehensively utilized with a biological bacterial liquid and an enzyme preparation. The biological bacterial liquid contains Bacillus licheniformis, Bacillus megaterium, Bacillus natto, Bacillus subtilis, Lactobacillus plantarum, Lactobacillus delbrueckii subspecies bulgaricus, Saccharomyces cerevisiae and Pichia pastoris. The enzyme preparation comprises amylase, cellulase, lipase, protease, pectinase and saccharifying enzyme. The method comprises the following steps: fermenting the kitchen waste, biological bacterial liquid and enzyme preparation in a fermentation tank at the constant temperature of 25-32 DEG C for 48-80 hours, distilling the fermentation broth, and rectifying to obtain the ethanol fuel.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and specifically relates to a method using biotechnology, that is, a biological bacterial liquid and an enzyme preparation are used to jointly treat kitchen wastes to produce fuel ethanol. Background technique: [0002] Food waste refers to leftovers, leftovers, roots and leaves of vegetables, skin cores of melons and fruits, discarded animal tissues, etc. Its components are mainly sugar, fat, protein, salt, water, etc., which are rich in nutrients. For a long time, food waste has been used as feed for pigs in our country, and it has found its own way through the market. There are many problems with this treatment method: 1. The waste has high moisture content, high fluidity, and is easy to leak, causing secondary pollution. 2. Garbage trucks affect the city's daily traffic and pollute the environment. 3. The garbage has not been treated in any way, and the hygiene of the pigs fed is worrying, which can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P7/08C12R1/10C12R1/11C12R1/07C12R1/125C12R1/25C12R1/225C12R1/84C12R1/865
Inventor 乔长昇李栋杨朋朋蒋磊赵男
Owner 天津北洋百川生物技术有限公司