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Well-type neurochip and preparation method thereof

A neural chip and nerve technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of small amplitude, distance and complex shape of electrical signals

Inactive Publication Date: 2012-02-22
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] But this kind of neural chip faces a problem: the nerve cells cultured in vitro will randomly adhere to the substrate, not accurately positioned on the electrode, resulting in poor adhesion or distance between most cells and the electrode, so electrical stimulation and Electrical signal detection may occur in different parts of different cells
Since the electrical signal recorded by the extracellular electrode is very sensitive to the spatial position relationship between the cell and the electrode, the amplitude of the obtained electrical signal is often small (especially for the recording of the neural network in the early developmental stage), and the shape is also complicated, so it is not conducive to Software sorting of electrical signals and determination of corresponding nerve cells

Method used

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  • Well-type neurochip and preparation method thereof

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Effect test

Embodiment 1

[0026] The well type neural chip of the present embodiment is made up of chip unit and a bottomless culture cavity (such as figure 1 shown). The chip unit is composed of a substrate, metal leads, microelectrodes, insulating layers and nerve wells; the substrate is glass; the metal leads are gold; there is a microelectrode array on the substrate, and the diameter of the microelectrodes is 50 μm. The quantity is 16, the distance between micro-electrodes is 500 μm, the surface of micro-electrodes is treated with platinum black, and the insulating layer is SiO 2 and Si 3 N 4 Each nerve well corresponds to a microelectrode; the wall of the nerve well is composed of chitosan porous network with a porosity greater than 90% and a pore size of 5 μm.

[0027] The preparation method of the well type neural chip is carried out according to the following steps:

[0028] 1) Preparation of the chip unit of the microelectrode array device

[0029] (1) SiO used 2 The base is cleaned;

...

Embodiment 2

[0045] The well-shaped neural chip of this embodiment is composed of a chip unit and a bottomless culture chamber. The chip unit is composed of a substrate, metal leads, microelectrodes, insulating layers and nerve wells; the substrate is glass; the metal leads are gold; there is a microelectrode array on the substrate, the diameter of the microelectrode is 30 μm, the microelectrode array is a lattice, and the microelectrode The quantity is 32, the distance between micro-electrodes is 300 μm, the surface of micro-electrodes is treated with platinum black, and the insulating layer is SiO 2 and Si 3 N 4 Each nerve well corresponds to a microelectrode; the wall of the nerve well is composed of chitosan porous network with a porosity greater than 90% and a pore size of 3 μm.

[0046] The preparation method of the well type neural chip is carried out according to the following steps:

[0047] 1) Preparation of the chip unit of the microelectrode array device

[0048] (1) SiO used...

Embodiment 3

[0064] The well-shaped neural chip of this embodiment is composed of a chip unit and a bottomless culture chamber. The chip unit is composed of a substrate, metal leads, microelectrodes, insulating layers and nerve wells; the substrate is glass; the metal leads are gold; there is a microelectrode array on the substrate, the diameter of the microelectrode is 30 μm, the microelectrode array is a lattice, and the microelectrode The quantity is 19, the distance between microelectrodes is 200μm, the surface of microelectrodes is treated with platinum black, and the insulating layer is SiO 2 and Si 3 N 4 Each nerve well corresponds to a microelectrode; the wall of the nerve well is composed of chitosan porous network with a porosity greater than 90% and a pore size of 4 μm.

[0065] The preparation method of the well type neural chip is carried out according to the following steps:

[0066] 1) Preparation of the chip unit of the microelectrode array device

[0067] (1) SiO used ...

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Abstract

The invention discloses a well-type neurochip and a preparation method thereof, belonging to the technical field of in vitro electrophysiological measurement equipment for nerves. The neurochip comprises a chip unit and a culture chamber, and the chip unit comprises a substrate, metal lead wires microelectrodes, an insulating layer and neural wells. The preparation method for the neurochip comprises the following steps: sputtering a gold coating on the substrate to form a metal lead wire layer; forming the insulating layer by using the method of plasma enhanced chemical vapor deposition; applying reactive ion etching on the insulating layer so as to allow the microelectrodes to be exposed; electroplating platinum-black on the microelectrodes; preparing polydimethylsiloxane dies over the microelectrodes, injecting a chitosan solution into the dies, carrying out freeze drying on the dies to obtain porous well type walls, and tearing off the polydimethylsiloxane dies to form the chip unit; allowing the culture chamber and the chip unit to be in seamless connection. According to the invention, a chitosan network structure of neural well walls allows neurites to pass through but does not allow nerve cells to pass through; therefore, the neurochip in the invention can be used for positioning electrophysiological measurement of nerve cells.

Description

technical field [0001] The invention belongs to the technical field of in vitro electrophysiological measuring equipment for nerves, and in particular relates to a well-shaped nerve chip and a preparation method thereof. Background technique [0002] Neurochip refers to the general term for micro-devices that can detect neurophysiological signals or neurotransmitters in multiple channels at the level of nerve cells, tissues or systems in real time. The earliest planar neurochip was reported by Thomas et al. in 1972 for recording in vitro culture. Electrical activity of cardiomyocytes. [0003] But this kind of neural chip faces a problem: the nerve cells cultured in vitro will randomly adhere to the substrate, not accurately positioned on the electrode, resulting in poor adhesion or distance between most cells and the electrode, so electrical stimulation and Electrical signal detection may occur in different parts of different cells. Since the electrical signal recorded by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N27/00
Inventor 王常勇韩尧王妍林秋霞陈威震杜芝燕段翠密
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA