Method for controlling growth of pipeline biological film by utilizing bromine chlorine hydantoin liposome

Abstract

A method for controlling the growth of a pipeline biological film by utilizing bromine chlorine hydantoin liposome comprises the following steps of: firstly, dissolving soybean lecithin and cholesterol into a chloroform and ether mixed solvent, adding BCDMH (Bromine Chlorine Dimethyl Hydantoin) acid salt buffer solution, carrying out water bath ultrasound treatment and forming stable emulsion; secondly, adding the emulsion into an eggplant-shaped bottle, placing the eggplant-shaped bottle on a rotary evaporator and evaporating the emulsion after pressure reduction to remove an organic solvent; thirdly, taking down the eggplant-shaped bottle, blowing by nitrogen, removing difficult-to-volatilize diethyl ether existing in the emulsion, continuing to evaporate, forming a uniform lipid membrane on the wall of the bottle, adding PBS (Phosphate Buffered Saline) and obtaining liposome suspension liquid after the membrane on the wall of the eggplant-shaped bottle is cleaned and removed; fourthly, filtering and removing large-particle impurities by using a micro-porous filtering membrane soaked in the PBS, placing at a temperature of 4 DEG C for storage and stripping the microbiological membrane of a simulated pipe network by the prepared bromine chlorine hydantoin liposome. The method has high embedding rate and high biological stripping efficiency and plays slow release and disinfection roles.

Description

[0001] technical field

[0002] The invention belongs to the field of water treatment, and relates to a biofilm control technology for drinking pipeline water, in particular to a method for controlling the growth of pipeline biofilm by utilizing a novel bromochlorohydantoin liposome embedding technology.

[0003] Background technique

[0004] Microorganisms in drinking water sources can attach and grow in drinking water pipe network systems with relatively low nutrient content, forming mature biofilms. Biofilms are mainly composed of polymers, proteins, and ribose substances, which can provide a "shelter" for biofilms to adhere tightly to the surface of the carrier, effectively protecting biofilms from various damages from the outside world. The biofilm in the drinking water pipeline system will cause the corrosion of the pipeline, and produce toxic and harmful microbial metabolites, produce malodorous gas, and reduce the water quality. Therefore, effective stripping ...

Examples

Embodiment 1

[0034] Accurately weigh 1.2g soybean lecithin and 0.6g cholesterol, dissolve in 10mL chloroform and 15 mL diethyl ether mixed solvent, add 6mL 2 g / L BCDMH phosphate buffer solution (PBS, pH=6.8, filter to remove suspended matter), Sonicate in a water bath for 0.5 min to form an emulsion. The emulsion was poured into an eggplant-shaped bottle and placed on a rotary evaporator. After the pressure was reduced to 0.02 MPa, the emulsion was evaporated at 30 °C for 8 h to remove the organic solvent. The rotation speed of the eggplant-shaped bottle was 100 r / min. Remove the eggplant-shaped bottle and blow off with nitrogen to remove the hard-volatile ether present in the emulsion. After continuing to evaporate for 8 hours, a uniform lipid film was formed on the wall of the bottle. Add 40 mL, 2 g / L PBS (pH=6.8) and continue to rotate for 6-9 hours. white) liposome suspension. The microporous membrane (particle size: 0.8 μm) soaked in PBS (pH=6.8) was used to filter and remove large ...

Embodiment 2

[0037] Weigh 1g soybean lecithin and 0.4g cholesterol, dissolve in 5mL chloroform and 10mL diethyl ether mixed solvent, add 8mL 2 g / L BCDMH phosphate buffer solution (PBS, pH=6.8, filter to remove suspended matter), water bath ultrasonic Treat for 1 min to form an emulsion. The emulsion was poured into an eggplant-shaped bottle and placed on a rotary evaporator. After the pressure was reduced to 0.04 MPa, the emulsion was evaporated at 37.5 °C for 12 h to remove the organic solvent. The rotation speed of the eggplant-shaped bottle was 135 r / min. Remove the eggplant-shaped bottle and blow off with nitrogen to remove the hard-volatile ether present in the emulsion. After continuing to evaporate for 12 hours, a uniform lipid film was formed on the wall of the bottle. Add 60mL, 2 g / L PBS (pH=6.8) and continue to rotate for 7.5 hours. liposome suspension. The microporous membrane (particle size: 0.8 μm) soaked in PBS (pH=6.8) was used to filter and remove large particles of impur...

Embodiment 3

[0040]Weigh 1.4 g soybean lecithin and 0.8 g cholesterol, dissolve in 15 mL chloroform and 20 mL diethyl ether mixed solvent, add 10 mL 2 g / L BCDMH phosphate buffer solution (PBS, pH=6.8, filter to remove suspended matter) , ultrasonically treated in a water bath for 2 min to form a stable emulsion. The emulsion was put into an eggplant-shaped bottle and placed on a rotary evaporator. After the pressure was reduced to 0.06 MPa, the emulsion was evaporated at 45 °C for 16 h to remove the organic solvent. The rotation speed of the eggplant-shaped bottle was 165 r / min. Remove the eggplant-shaped bottle and blow off with nitrogen to remove the hard-volatile ether present in the emulsion. After continuing to evaporate for 16 hours, a uniform lipid film was formed on the wall of the bottle. Add 80 mL, 2 g / L PBS (pH=6.8) and continue to rotate for 9 hours. liposome suspension. The microporous membrane (particle size: 0.8 μm) soaked in PBS (pH=6.8) was used to filter and remove larg...

[0001] technical field

[0002] The invention belongs to the field of water treatment, and relates to a biofilm control technology for drinking pipeline water, in particular to a method for controlling the growth of pipeline biofilm by utilizing a novel bromochlorohydantoin liposome embedding technology.

[0003] Background technique

[0004] Microorganisms in drinking water sources can attach and grow in drinking water pipe network systems with relatively low nutrient content, forming mature biofilms. Biofilms are mainly composed of polymers, proteins, and ribose substances, which can provide a "shelter" for biofilms to adhere tightly to the surface of the carrier, effectively protecting biofilms from various damages from the outside world. The biofilm in the drinking water pipeline system will cause the corrosion of the pipeline, and produce toxic and harmful microbial metabolites, produce malodorous gas, and reduce the water quality. Therefore, effective stripping ...

Examples

Embodiment 1

[0034] Accurately weigh 1.2g soybean lecithin and 0.6g cholesterol, dissolve in 10mL chloroform and 15 mL diethyl ether mixed solvent, add 6mL 2 g / L BCDMH phosphate buffer solution (PBS, pH=6.8, filter to remove suspended matter), Sonicate in a water bath for 0.5 min to form an emulsion. The emulsion was poured into an eggplant-shaped bottle and placed on a rotary evaporator. After the pressure was reduced to 0.02 MPa, the emulsion was evaporated at 30 °C for 8 h to remove the organic solvent. The rotation speed of the eggplant-shaped bottle was 100 r / min. Remove the eggplant-shaped bottle and blow off with nitrogen to remove the hard-volatile ether present in the emulsion. After continuing to evaporate for 8 hours, a uniform lipid film was formed on the wall of the bottle. Add 40 mL, 2 g / L PBS (pH=6.8) and continue to rotate for 6-9 hours. white) liposome suspension. The microporous membrane (particle size: 0.8 μm) soaked in PBS (pH=6.8) was used to filter and remove large ...

Embodiment 2

[0037] Weigh 1g soybean lecithin and 0.4g cholesterol, dissolve in 5mL chloroform and 10mL diethyl ether mixed solvent, add 8mL 2 g / L BCDMH phosphate buffer solution (PBS, pH=6.8, filter to remove suspended matter), water bath ultrasonic Treat for 1 min to form an emulsion. The emulsion was poured into an eggplant-shaped bottle and placed on a rotary evaporator. After the pressure was reduced to 0.04 MPa, the emulsion was evaporated at 37.5 °C for 12 h to remove the organic solvent. The rotation speed of the eggplant-shaped bottle was 135 r / min. Remove the eggplant-shaped bottle and blow off with nitrogen to remove the hard-volatile ether present in the emulsion. After continuing to evaporate for 12 hours, a uniform lipid film was formed on the wall of the bottle. Add 60mL, 2 g / L PBS (pH=6.8) and continue to rotate for 7.5 hours. liposome suspension. The microporous membrane (particle size: 0.8 μm) soaked in PBS (pH=6.8) was used to filter and remove large particles of impur...

Embodiment 3

[0040]Weigh 1.4 g soybean lecithin and 0.8 g cholesterol, dissolve in 15 mL chloroform and 20 mL diethyl ether mixed solvent, add 10 mL 2 g / L BCDMH phosphate buffer solution (PBS, pH=6.8, filter to remove suspended matter) , ultrasonically treated in a water bath for 2 min to form a stable emulsion. The emulsion was put into an eggplant-shaped bottle and placed on a rotary evaporator. After the pressure was reduced to 0.06 MPa, the emulsion was evaporated at 45 °C for 16 h to remove the organic solvent. The rotation speed of the eggplant-shaped bottle was 165 r / min. Remove the eggplant-shaped bottle and blow off with nitrogen to remove the hard-volatile ether present in the emulsion. After continuing to evaporate for 16 hours, a uniform lipid film was formed on the wall of the bottle. Add 80 mL, 2 g / L PBS (pH=6.8) and continue to rotate for 9 hours. liposome suspension. The microporous membrane (particle size: 0.8 μm) soaked in PBS (pH=6.8) was used to filter and remove larg...

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