Preparation method of protein stored in camellia oleifera abel cakes

A technology of protein and camellia oleifera cake, which is applied in the field of preparation of stored protein in camellia oleifera cake, can solve the problems of extracting high-quality protein from camellia oleifera cake, achieve high extraction rate, good quality, and improve the effect of resource utilization

Inactive Publication Date: 2012-02-29
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

But, up to now, do not see as yet the report of extracting the high-quality protein wherein with camellia oleifera cake

Method used

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  • Preparation method of protein stored in camellia oleifera abel cakes

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example 1

[0031] Now take the laboratory implementation as an example, and the non-limiting examples are described as follows:

[0032] Dry the tea seed cake raw material after oil extraction under vacuum conditions at 40-65°C and crush it; take 200g of the raw material that has been pulverized and completely degreased, add petroleum ether that is 4-5 times the weight of the oil tea cake powder, and heat it with petroleum ether Reflux for 7-10 hours, remove grease, separate and filter, evaporate the residual solvent, add NaOH solution with a pH of 10 according to the ratio of material to liquid 1:30, and soak at room temperature for 60-100min; Centrifuge under the condition of 1 / min for 20min, take the supernatant as the rough separation extract, adjust the pH value of the rough separation extract with 1mol / L HCl solution, make it reach the isoelectric point 5 of camellia oleifera protein, and keep the isoelectric point state Under these conditions, the protein precipitate was obtained ...

example 2

[0037] Dry the tea seed cake raw material after oil extraction under vacuum conditions at 40-65°C, and crush it; take 200g of the raw material that has been pulverized and completely degreased, add petroleum ether 4-5 times the weight of the oil tea cake powder and 2-4 grams For citric acid, heat and reflux with petroleum ether for 7-10 hours to remove grease, separate and filter to evaporate the residual solvent, add NaOH solution with a pH of 10 according to the ratio of material to liquid 1:30, and soak at room temperature for 60-100 minutes; the crude extract Centrifuge at 4°C for 20min at a speed of 6000r / min, take the supernatant as the crude separation extract, adjust the pH of the crude separation extract with 1mol / L HCl solution, and make it reach the isoelectric point of the camellia oleifera protein 5. Under the condition of maintaining the isoelectric point state, refrigerate and centrifuge to obtain the protein precipitate. Put the precipitate in deionized water f...

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Abstract

The invention provides a preparation method of protein stored in camellia oleifera abel cakes. According to the method, camellia oleifera abel cakes are adopted as a raw material. The preparation method of protein stored in the camellia oleifera abel cakes comprises steps that: a camellia oleifera abel cake raw material is processed through an appropriate pretreatment after oil extraction; the material is processed through alkali extraction; the extract is processed through frozen centrifugation; an obtained supernatant is processed through acid precipitation and frozen centrifugation; an obtained precipitate is processed through dialysis; an obtained product is processed through vacuum lyophilisation, such that the protein is obtained. The protein is then processed through functional property researches and evaluations. The extraction method provided by the invention is advantaged in simple technology, low processing cost, and high extraction rate. With the method, camellia oleifera abel resources can be fully utilized.

Description

technical field [0001] The invention relates to a method for extracting and purifying protein, in particular to a method for preparing protein stored in camellia oleifera cake. Background technique [0002] Camellia oleifera (Camellia oleifera Abel), also known as tea seed tree, camellia oleifera, and white flower tea, belongs to the genus Camellia in the family Theaceae. It is an important oil plant in the hilly areas of southern my country, mainly distributed in Hunan, Jiangxi, Guangxi and other provinces. Camellia oleifera seed is the seed of camellia oleifera tree, with an oil content of more than 30%. The by-product of camellia oleifera after pressing——camellia oleifera cake contains tea saponin, tea seed polysaccharide, tea seed protein, etc. They are all used in chemical industry, light industry, Raw materials for food, feed industrial products, etc., have high economic value. [0003] Camellia oleifera cake is rich in protein and has high nutritional value. Its comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/30C07K1/14
Inventor 丁之恩聂芸丁昱周倩
Owner ANHUI AGRICULTURAL UNIVERSITY
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