Microbacterium 18 for degrading unsymmetrical dimethylhydrazine and unsymmetrical dimethylhydrazine method therewith

A technology of unsymmetrical dimethylhydrazine and microbacteria, which is applied in the biological field and can solve problems such as high operating costs, high energy consumption, and immature technology

Inactive Publication Date: 2012-03-07
LOGISTICS DEPT ANTIEPIDEMIC ARMY OF THE GENERAL ARMAMENT DEPT PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These unsymmetrical dimethylhydrazine treatment methods have problems such as high energy consumption, high operating costs, low safety factor, and immature technology.

Method used

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  • Microbacterium 18 for degrading unsymmetrical dimethylhydrazine and unsymmetrical dimethylhydrazine method therewith

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The present embodiment provides a kind of method utilizing Microbacterium M18 to degrade unsymmetrical dimethylhydrazine, comprising:

[0018] Seed culture: inoculate the strain of Microbacterium M18 on a slant culture medium, and culture at 24-37° C. for 12-30 hours to obtain a slant culture. The above slant medium consists of: tryptone 1-15g / L, malt extract 1-15g / L, bacto-peptone 1-15g / L and agar 15g / L.

[0019] Take the well-grown slant culture and inoculate it into a 250mL Erlenmeyer flask containing 50-100mL of seed medium. Under the condition that the rotational speed of the shake flask is 150-200 r / min and the culture temperature is 28-37 DEG C, culture is carried out for 12-30 hours to obtain the seed culture liquid. The composition of the above-mentioned seed medium: 1-15 g / L of tryptone, 1-15 g / L of malt extract and 1-15 g / L of bacto-peptone.

[0020] Degradation of unsymmetrical dimethylhydrazine: Inoculate 15.78mL of seed liquid into a 1000mL Erlenmeyer fl...

Embodiment 2

[0023] Seed culture: inoculate the strain of Microbacterium M18 on a slant medium composed of tryptone, malt extract, bacto-peptone, and agar, and cultivate it at 27°C for 24 hours to obtain a slant culture; the composition of the above slant medium : tryptone 3g / L, malt extract 3g / L, bacto-peptone 5g / L and agar 15g / L.

[0024] Take the well-grown slant culture and inoculate it into a 250mL Erlenmeyer flask containing 110mL of seed medium, and cultivate it for 18 hours under the conditions of a shaker flask rotation speed of 200r / min and a culture temperature of 30°C; the composition of the above seed medium: Peptone 3g / L, malt extract 3g / L and bacto-peptone 5g / L.

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Abstract

The invention discloses a microbacterium 18 for degrading unsymmetrical dimethylhydrazine, and provides a method for degrading unsymmetrical dimethylhydrazine with the microbacterium 18. The method comprises the steps of: inoculating a microbacterium 18 strain in a slant culture medium composed of tryptone, malt extract, bacto peptone and agar for culturing so as to obtain a slant culture; inoculating the slant culture in a container loaded with a seed culture medium composed of tryptone, malt extract, and bacto peptone for culturing so as to obtain a seed solution; inoculating the seed solution in a container loaded with a substrate solution composed of cane sugar, NaCl, K2HPO4, KH2PO4, MgSO4, CaCl2, FeSO4, H3BO3, ZnSO4 and unsymmetrical dimethylhydrazine for culturing, and conducting degradation at a temperature of 28-37DEG C for 72h. With the microbacterium 18 and the method that are provided in the invention, the degradation rate of unsymmetrical dimethylhydrazine can reach 88.06%.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a microbacterium M18 degrading unsymmetrical dimethylhydrazine and a method for degrading unsymmetrical dimethylhydrazine. Background technique [0002] Unsymmetrical dimethylhydrazine, the English name is Unsymmetrical Dimethylhydrazine, referred to as UDMH, and the molecular formula is (CH 3 ) 2 NNH 2 , the molecular weight of unsymmetrical dimethylhydrazine is 60.11, the boiling point is 63°C, the freezing point is -57.2°C, and the density is 0.791g / cm 3 , has good hydrophilicity, is miscible with water, and can be mixed with gasoline, alcohol and other organic solvents. Unsymmetrical dimethylhydrazine is a flammable, volatile, colorless or slightly yellowish transparent liquid with a strong pungent fishy odor. It has high calorific value and good combustion performance, and is a rocket propellant with excellent performance. my country's Long March series of rockets use unsym...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/26
Inventor 王力夏本立丛继信范春华曹晔彭清涛曹巧玲
Owner LOGISTICS DEPT ANTIEPIDEMIC ARMY OF THE GENERAL ARMAMENT DEPT PLA
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