Embedded tissue-specific expressed Cre tool mice built by utilizing Flp-Frt system

A flp-frt, cd4-stop-cre technology, applied in the biological field, can solve the problems such as not many Cre transgenic tool mice, incomplete tissue-specific promoters, affecting expression, etc.
CN102373237AInactive Publication Date: 2012-03-14NANJING UNIV SCI PARK DEV
1 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING UNIV SCI PARK DEV
Publication Date
2012-03-14
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

Traditional Cre transgene has difficultly in carrying out tissue-specific expression. The invention carries out innovation based on a double transgenic technique and a Flp-Frt technique. The invention is characterized in that: firstly, an STOP original part is added in front of a translation initiation codon of Cre to inhibit the expression of downstream Cre, and two Frt sequences are separately added to the upstream and downstream of the STOP original part; then the STOP original part between the two Frt sequences can be specifically deleted by utilizing Flp recombinase, thus the downstream Cre recombinase can be expressed at the moment; and tissue-specific promoters are added in front of the expression plasmids of Cre and Flp, thus the tissue Cre which is expressed in both the promoters can be expressed. The invention has the beneficial effects that: improvement and innovation are carried out on a traditional building method of a tissue-specific Cre transgenic carrier; and the correct tissue specificity and time specificity of Cre are calibrated by utilizing an intersection of two specific promoters, thus promoting the research on gene function utilizing conditional gene knockout mice and further being applied to research of medical and pharmaceutical targets.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the establishment of a new nested tissue-specific Cre tool mouse, in particular to a new technology for establishing a nested tissue-specific expression Cre tool mouse by utilizing the Flp-Frt system and double transgenic technology, and belongs to the field of biotechnology . Background technique

[0002] Conditional gene knockout technology is an important means to study gene function and further apply to medical research. Tissue-specific expression of Cre transgenic mice is an important tool for conditional gene knockout technology and is widely used in scientific research and medical research. Cre recombinase was discovered by Sternberg in P1 phage in 1981. The gene has 1029 bases (Gene ID: 2777477), encodes a protein with a size of about 38K, belongs to the λInt enzyme superfamily (integrase superfamily), and is a site-specific recombinase that can recognize partially palindromic loxP sequences (5'-ATAACTTCGTATA-ATGTATG...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More