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Method for preparing citric acid through fermentation
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A technology of citric acid and fermentation bacteria, which is applied in the field of fermentation to produce citric acid, can solve problems such as energy waste, and achieve the effects of reducing energy consumption, solving emission problems and improving acid production rate.
Inactive Publication Date: 2013-10-30
COFCO BIOTECHNOLOGY CO LTD
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Abstract
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Problems solved by technology
As a result, the increased energy consumption due to oxygen supply in the citric acid fermentation process accounts for a large proportion of the production cost, and the direct discharge of fermentation tail gas into the air is also a waste of energy
Method used
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Effect test
Embodiment 1
[0048] 1) Put 28m into the seed tank 3 After the enzymolysis solution obtained above, the seed medium of the seed tank was sterilized at 121°C for 30 minutes, cooled to 36°C and inserted into Aspergillus niger seeds, and the inoculation amount was 2×10 per milliliter of enzymolysis solution. 5 colony-forming unit, at a temperature of 36°C, a ventilation rate of 0.3 volume: (volume · minute) of air (provided by the first compressor (air compressor), pressure 0.23MPa), and a tank pressure of 0.1MPa for 27 days. Hour, obtain the mature seed liquid and the first tail gas of cultivation, the first tail gas is passed through the pipe net with filter screen (filter screen aperture is 1um), enters the second compressor after passing through the filter again, controls the pressure of compressed tail gas It is 0.23MPa, and the compressed tail gas is stored in the gas storage tank.
[0049] 2) The enzymolysis solution obtained above is dropped into the fermenter for 20m 3 , the remaini...
Embodiment 2
[0053] 1) Put 28m into the seed tank 3 After the enzymolysis solution obtained above, sterilize the seed medium of the seed tank at 121°C for 30 minutes, cool down to 36°C and insert Aspergillus niger seeds, and the inoculation amount is 10 per milliliter of enzymolysis solution. 5 colony forming unit, at a temperature of 36°C, a ventilation rate of 0.45 volume: (volume · minute) of air (provided by the first compressor (air compressor), pressure 0.23MPa), and a tank pressure of 0.1MPa. Hour, obtain the mature seed liquid and the first tail gas of cultivating, the first tail gas is passed through the pipe network that has filter screen (filter screen aperture is 0.5um), enters the second compressor after passing through the filter again, controls the tail gas volume after compression The pressure is 0.23MPa, and the compressed tail gas is stored in the gas storage tank.
[0054] 2) The enzymolysis solution obtained above is dropped into the fermenter for 20m 3 , the remainin...
Embodiment 3
[0056] Adopt the method of embodiment 2 to carry out, difference is that in step 2), the time that starts feeding compressed tail gas is when fermenting 10 hours. Fermentor 56 hours sugar exhaustion stops fermentation, obtains citric acid fermented liquid, and the acidity of this citric acid fermented liquid, volume, compressor power consumption (comprising the electric energy consumed by the tail gas produced by compressing seed cultivation) are shown in Table 1.
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Abstract
The invention provides a method for preparing citric acid through fermentation, which comprises the steps that: under the condition of introducing first gas, citric acid zymogen is introduced into seed culture media in a seed tank for carrying out seed culture, seed culture liquid and first tail gas are obtained, under the condition of introducing second gas, the seed culture liquid is introduced into fermentation culture media in a fermentation culture tank for carrying out fermentation culture, and citric acid fermentation liquid and second tail gas are obtained, wherein the first gas is air, partial or all second gas is the first tail gas and / or the second tail gas, and in addition, the rest part of the second gas is air. When the method provided by the invention is adopted, the tail gas generated in the citric acid fermentation process can be effectively utilized, and in addition, the increased energy consumption of the citric acid fermentation caused by oxygen supply is also greatly reduced, so the production cost of the citric acid is reduced.
Description
technical field [0001] The invention relates to a method for fermenting citric acid. Background technique [0002] Citric acid is the largest acid among organic acids. Due to its excellent physical and chemical properties, it is widely used in medicine, chemistry, electronics, textiles, petroleum, leather, construction, photography, plastics, casting and ceramics and other industrial fields. [0003] The current mainstream technology for citric acid fermentation is two-stage liquid submerged fermentation technology, which requires a large amount of compressed air (0.18-0.3MPa) to be introduced during the fermentation process, and the tank pressure of the seed tank and fermentation tank is controlled at 0.05-0.1MPa Between to supply the growth and fermentation acid production of Aspergillus niger seeds. Because Aspergillus niger is more sensitive to oxygen during the growth and acid production stages, under the condition of insufficient dissolved oxygen or hypoxia, the metab...
Claims
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Application Information
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