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Application of 2 alpha-hydroxy protopanoxadiol medicine

A panaxadiol and drug technology, which is applied to the application field of 2α-hydroxy protopanaxadiol in the preparation of antitumor drugs, can solve few problems and the like, and achieve the effects of inhibiting proliferation and inhibiting tumor significantly.

Inactive Publication Date: 2012-04-04
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies and reports on the function of 2α-hydroxyprotopanaxadiol, especially the clear medicinal function

Method used

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  • Application of 2 alpha-hydroxy protopanoxadiol medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Determination of the inhibitory effect of 2α-hydroxyprotopanaxadiol on the growth of pancreatic cancer cells by MTS method

[0035] 3000 PANC-1 cells (purchased from the Chinese Academy of Sciences Cell Bank) were inoculated into 96-well plates, cultured for 24 hours to allow them to adhere to the wall, and then 2α-hydroxyprotopanaxadiol (purchased from the Shanghai Institute of Materia Medica, Chinese Academy of Sciences) was added. 6 concentration gradients, with 3 replicate wells for each concentration. Cells at 37°C, 5% CO 2 After culturing for 72 hours under the same conditions, the culture medium was discarded, and the cell viability was measured with an MTS kit (Promega).

[0036] The test method is: wash the cells once with serum-free medium, and add the pre-prepared MTS chromogenic solution according to the amount of 100 μl / well (add 2ml of solution 1 and 100μl of solution 2 to 10ml of serum-free medium, and mix well). A well without cells was set a...

Embodiment 2

[0039] Example 2 Growth inhibitory effect of 2α-hydroxyprotopanaxadiol on human leukemia cells

[0040] Using cck-8 kit (Japan Dojin Chemical Research Institute) detection.

[0041] steps:

[0042] 1) K562 cells (purchased from ATCC) were evenly seeded in a 96-well plate, with 10,000 cells per well.

[0043] 2) Leave to adhere to the wall, add drugs after overnight, add drugs (2α-hydroxyprotopanaxadiol concentrations are 50, 16.67, 5.56, 1.85, 0.62 μM, and each concentration has 3 replicate wells.

[0044] 3) After culturing for 48 hours, replace the complete medium with a mixture of serum-free medium and CCK8 (10:1), and incubate in a 37°C incubator for 2 hours.

[0045] 4) Take 450nm as the measurement wavelength and 650nm as the reference wavelength, and measure the reading on a microplate reader.

[0046] Results: The IC50 value of 2α-hydroxyprotopanaxadiol on k562 cells was 4.77μM.

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Abstract

The invention belongs to the fields of chemical industries and medicines, and relates to the application of 2 alpha-hydroxy protopanoxadiol in preparation of antitumor medicines. Antitumor cells comprise leukemia or pancreatic cancers. The 2 alpha-hydroxy protopanoxadiol belongs to a natural product and has certain clinical application value. A small molecular compound is used as a new antitumor medicine or an auxiliary component of the new antitumor medicine for development, has an obvious antitumor effect, is environment-friendly and provides a new method for treating and healing tumors.

Description

technical field [0001] The invention belongs to the fields of chemical industry and medicine, and relates to the application of 2α-hydroxy protopanaxadiol in the preparation of antitumor drugs. Background technique [0002] 2α-Hydroxy protopanaxadiol has the following structural formula. [0003] [0004] 1999 [0005] In 1998, Cui Jian-Fang et al prepared 2α-hydroxy protopanaxadiol (compound 6 in Table 1, Alkaline cleavage of gypenosides and characterization of dammarane-type aglycons by gas chromatography-mass spectrometry.Phytochemical Analysis. 9(3), 128-133). In 1999, Cui et al. reported a method for separating and identifying 2α-hydroxyl protopanaxadiol from Gynostemma pentaphyllum (compound 7, Gynostemma pentaphyllum: identification of major sapogenins and differentiation from Panax species. European Journal of Pharmaceutical Sciences.8(3), 187-191). However, there are few studies and reports on the function of 2α-hydroxyprotopanaxadiol, especially the clear me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/575A61P35/00A61P35/02C12Q1/02
Inventor 余龙唐丽莎刘祖龙胡立宏
Owner FUDAN UNIV
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