Preparation and quality detection methods of high-purity honeysuckle flower-baikal skullcap root soluble powder

A quality detection method and soluble technology, which are applied in the field of preparation and quality detection of high-purity silver yellow soluble powder, can solve the problems of high cost, unfavorable promotion, development and application, complicated methods, etc., and achieve reduced application and reliable quality control method. , good water solubility

Inactive Publication Date: 2012-04-04
BEIJING CENT BIOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there are many reports related to the preparation method of Yinhuang preparation, the method is more complicated and the cost is high, which is not conducive to popularization, development and application

Method used

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  • Preparation and quality detection methods of high-purity honeysuckle flower-baikal skullcap root soluble powder
  • Preparation and quality detection methods of high-purity honeysuckle flower-baikal skullcap root soluble powder
  • Preparation and quality detection methods of high-purity honeysuckle flower-baikal skullcap root soluble powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Take 375g of Scutellaria baicalensis Georgi, add water and decoct four times, combine the decoction, filter, and concentrate the filtrate to a relative density of 1.10; adjust the pH to 1.7 with hydrochloric acid, keep it at 60°C, let it stand, and filter; add appropriate amount of water to the precipitate and stir evenly , Adjust the pH value to 6.5 with 40% sodium hydroxide; add the same amount of ethanol, stir to dissolve, filter, adjust the pH value of the filtrate to 1.7 with hydrochloric acid, keep it at 70°C, keep it, and filter; Wash with different concentrations of ethanol to pH 7.0; evaporate the ethanol and dry under reduced pressure to obtain 29.6 g of Scutellaria baicalensis extract; take 500 g of honeysuckle and decoct it once with water; combine the decoction and concentrate the filtrate under reduced pressure to a relative density of 1.10 ( 70℃) cleansing paste; add ethanol to make the alcohol content reach 95%, stand for 24 hours, and filter; add ethanol ...

Embodiment 2

[0038] 1. For identification, take 1g of silver yellow soluble powder, add 9ml of 75% ethanol, shake it well, and use it as the test solution; take baicalin reference substance and chlorogenic acid reference substance, respectively add 75% ethanol to make each 1ml containing 0.3 mg solution, as the reference solution; according to the thin-layer chromatography test, draw 2μl of each of the above three solutions, respectively dot on the same polyamide film, use acetic acid as a developing agent, unfold, take out, dry, and place a UV lamp ( Check at 365nm); in the chromatogram of the test substance, the same color fluorescent spots appear on the position corresponding to the chromatogram of the reference substance.

[0039] 2. Inspect, the moisture content shall not exceed 5.0% according to the moisture determination method; others, except for the content uniformity, shall comply with the various regulations under the soluble powder item.

[0040] 3. Assay, honeysuckle, according to ...

Embodiment 3

[0044] Example 3: Research on the preparation and quality detection methods of silver yellow soluble powder and experimental research on the extraction process of scutellaria in silver yellow soluble powder

[0045] Scutellaria Baicalensis Georgi (Sctellaria Baicalensis Georgi) is a commonly used traditional Chinese medicine. Its main component is flavonoids. Among them, Baicalin has the highest content, which has good extraction and utilization value. Pharmacologically, it has been confirmed that it has the functions of antibacterial, clearing away heat, diuresis, choleretic, anti-inflammatory, anti-allergic, and detoxification.

[0046] In this experiment, baicalin can be easily extracted and purified by taking advantage of the easy precipitation of baicalin in acidic solution.

[0047] 1 Instruments and reagents

[0048] 1.1 Instrument SHIMADZU L VP C-10AT high performance liquid chromatograph; SPD-10A VP UV-Vis monitor; Chromatographic column: SHIMADZU ODSC18 column (150×4.6mm, 5μ...

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Abstract

The invention discloses preparation and quality detection methods of high-purity honeysuckle flower-baikal skullcap root soluble powder. The preparation method comprises the following steps of: 1, preparing an appropriate amount of baikal skullcap root, adding water for decocting for 2-4 times, combining decoctions, filtering, and concentrating the filtrate till the relative density is 1.10-1.35; 2, regulating the pH value to 1.0-2.0 with hydrochloric acid, preserving heat at the temperature 60-80 DEG C, standing, and filtering; 3, adding water into a precipitate, stirring uniformly, and regulating the pH value to 6.5-7.5 with 30-40 percent sodium hydroxide; 4, adding an equal amount of ethanol, stirring for dissolving, filtering, regulating the pH value of the filtrate to 1.0-2.0 with hydrochloric acid, preserving heat at the temperature 60-80 DEG C, standing, and filtering; 5, washing the precipitate with an appropriate amount of water and ethanol of different concentrations in sequence till the pH value is 6.5-7.5; 6, vaporizing the ethanol and drying under reduced pressure to obtain a baikal skullcap root extract; 7, preparing an appropriate amount of honeysuckle flower, adding water for decocting for 1-3 times, combining decoctions, filtering, and concentrating the filtrate under reduced pressure at the temperature 50-70 DEG C till the relative density is 1.10-1.25 to obtain clear paste; 8, adding ethanol till the ethanol content is 80-90 percent, standing for 24 hours, and filtering; 9, adding ethanol into filter residues till the ethanol content is 75-95 percent, standing for 18-30 hours, and filtering; 10, combining two filtrates, recovering ethanol, and concentrating under reduced pressure till the relative density is 1.15-1.30; 11, performing spray drying to obtain a honeysuckle flower extract; and 12, combining the baikal skullcap root extract with the honeysuckle flower extract, adding sodium bicarbonate to fixed amount, adding glucose to fixed amount, and mixing uniformly to obtain the honeysuckle flower-baikal skullcap root soluble powder.

Description

Technical field [0001] The invention relates to a high-purity silver-yellow soluble powder, and a preparation and quality detection method of the high-purity silver-yellow soluble powder. Background technique [0002] Yinhuang preparations are mainly composed of honeysuckle and scutellaria. It has the effects of clearing away heat, detoxifying, antibacterial and anti-inflammatory. It is one of the traditional medicines with a long history in my country. In the research and application at home and abroad, it has found its various pharmacological and clinical functions. Veterinarians are mainly used in livestock and poultry for exogenous fever, pneumonia, asthma and mixed infections caused by multiple bacteria. In addition, some data prove that its antibacterial and anti-inflammatory effects are significant, and it has obvious antibacterial effects on Proteus, Staphylococcus aureus, Staphylococcus aureus, Streptococcus A, and Streptococcus B; it also has a significant inhibitory eff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/539A61K9/14G01N30/90G01N30/02A61P29/00A61P39/02A61P11/10A61P11/14A61P11/06A61P1/12A61P31/04A61P31/12A61P37/04A61P9/14A61P1/16
Inventor 江厚生王秀敏侯晓礁李学良
Owner BEIJING CENT BIOLOGY CO LTD
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