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Background image processing method of slices under microscope

A background image and microscopic technology, applied in the field of image processing, can solve the problems of high hardware cost, affecting the result of image background correction, practical limitations, etc., and achieve high processing speed, good processing effect and wide application range Effect

Active Publication Date: 2012-04-18
MOTIC CHINA GRP CO LTD
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Problems solved by technology

The above-mentioned software background correction method has a major disadvantage to the conventional image light field uniform correction method: the image used for background image correction must be absolutely blank, and only reflect the uneven change of microscope illumination, but it is difficult to do it in reality. When the background image is used, the aligned field of view will always have more or less dirty spots or noises, which will directly affect the results of image background correction
The advantage of this method is that it can remove the noise and dirty points of the background correction image, but the disadvantage is that the hardware cost is high and the configuration is complicated, so it also has limitations in practicability.

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  • Background image processing method of slices under microscope
  • Background image processing method of slices under microscope
  • Background image processing method of slices under microscope

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Experimental program
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Embodiment 1

[0023] Such as figure 1 As shown, the background image processing method of a slice under a microscope according to the present invention includes the following steps:

[0024] Step 100: Grab an original background image block in a field of view of the microscope.

[0025] After adjusting the eyepiece and objective lens of the microscope, aim the camera at a blank field of view, and the image observed under the eyepiece of the microscope is the original background image block in the one field of view of the microscope, and the background image block may If there is noise or dirty spots, grab the image block as the original background image block. Such as Figure 4 As shown, on the profile analysis curve on the left side of the figure, there are many noises or stains on the original background image block.

[0026] In step 200, a mathematical model is selected to fit the original background image block, and specific parameter values ​​of the mathematical model are calculated accordin...

Embodiment 2

[0088] The difference between this embodiment and the first embodiment is that the first embodiment uses a Gaussian mathematical model to process the original background image, and this embodiment uses a polynomial nonlinear mathematical model to fit the original background image. The parts of this embodiment that are the same as the first embodiment will not be described in detail below.

[0089] For a monochrome grayscale camera, the third-order nonlinear model formula is:

[0090] G(x,y)=Ax 3 +Bx 2 y+Cxy 2 +Dy3+Ex 2 +Fxy+Gy 2 +Hx+Iy+J

[0091] Among them, G (x, y) is the gray value of each pixel of the two-bit image matrix, x is the horizontal position of the two-bit image matrix (the value range is the image horizontal position 1 to M), and y is the two-bit The vertical position of the image matrix (the value range is the image vertical position 1 to N), so the total number of pixels is MxN. A, B, C, D, E, F, G, H, I, J are the 10 parameters of the third-order nonlinear model f...

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Abstract

The invention discloses a background image processing method of slices under a microscope. The background image processing method comprises the following steps of: capturing an original background image block in a vision field of the microscope; fitting the original background image block by using a mathematical model, and figuring out specific parameter values of the mathematical model according to the original background image block; and figuring out a new background image block according to physical ratio size of the original background image block and the specified mathematical model so as to replace the original background image block. By adopting the technical scheme, the requirement on the adopted original background image block is lower, and the original background image block is not necessarily an absolute blank background image; and furthermore, the processing effect of the background image is good.

Description

Technical field [0001] The invention relates to an image processing method, in particular to a background image processing method of slices under a microscope. Background technique [0002] A microscope digital imaging system will always produce uneven images due to the influence of the illumination unit and the optical system. Typically, the digital image is brighter in the middle and darker on the edges. The unevenness of the image light field has a great influence on the observation of the imaged image, especially for digital slice scanning. In digital slice scanning, images need to be stitched. If the brightness values ​​of the left and right or top and bottom images change slightly, such as a change of more than 5 gray levels, the stitched image will have obvious brightness stitching traces. At present, there are mainly software background correction methods and hardware background correction methods for light field correction of digital microscope imaging. [0003] The soft...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T5/00
Inventor 肖倩陈德敏陈进
Owner MOTIC CHINA GRP CO LTD
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