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Background image processing method of slices under microscope

A background image and microscope technology, applied in the field of image processing, can solve the problems of affecting the result of image background correction, high hardware cost, and practical limitations, and achieve good processing effect, high processing speed, and wide application range Effect

Active Publication Date: 2014-11-26
MOTIC CHINA GRP CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned software background correction method has a major disadvantage to the conventional image light field uniform correction method: the image used for background image correction must be absolutely blank, and only reflect the uneven change of microscope illumination, but it is difficult to do it in reality. When the background image is used, the aligned field of view will always have more or less dirty spots or noises, which will directly affect the results of image background correction
The advantage of this method is that it can remove the noise and dirty points of the background correction image, but the disadvantage is that the hardware cost is high and the configuration is complicated, so it also has limitations in practicability.

Method used

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  • Background image processing method of slices under microscope
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  • Background image processing method of slices under microscope

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Experimental program
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Embodiment 1

[0023] Such as figure 1 As shown, a background image processing method of a section under a microscope according to the present invention comprises the following steps:

[0024] Step 100, capturing an original background image block under a field of view of the microscope.

[0025] After adjusting the eyepiece and objective lens of the microscope, aim the camera at a blank field of view, the image observed under the eyepiece of the microscope is the original background image block under the field of view of the microscope, and the background image block may be There are noises or dirty points, grab this image block as the original background image block. Such as Figure 4 As shown, on the profile analysis curve on the left side of the figure, there are many noises or stains on the original background image block.

[0026] Step 200, selecting a mathematical model to fit the above-mentioned original background image block, and calculating specific parameter values ​​of the ma...

Embodiment 2

[0088] The difference between this embodiment and the first embodiment is that the first embodiment uses a Gaussian mathematical model to process the original background image, while this embodiment uses a polynomial nonlinear mathematical model to fit the original background image. The parts of this embodiment that are the same as those of Embodiment 1 will not be repeated below.

[0089] For a monochrome grayscale camera, the third-order nonlinear model formula is:

[0090] G(x,y)=Ax 3 +Bx 2 y+Cxy 2 +Dy3+Ex 2 +Fxy+Gy 2 +Hx+Iy+J

[0091] Among them, G(x, y) is the gray value of each pixel of the binary image matrix, x is the horizontal position of the binary image matrix (the value range is image horizontal position 1 to M), and y is the binary image matrix The vertical position of the image matrix (the value ranges from the image vertical position 1 to N), so the total number of pixels is MxN. A, B, C, D, E, F, G, H, I, J are 10 parameters of the 3rd degree nonlinear ...

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Abstract

The invention discloses a background image processing method of slices under a microscope. The background image processing method comprises the following steps of: capturing an original background image block in a vision field of the microscope; fitting the original background image block by using a mathematical model, and figuring out specific parameter values of the mathematical model according to the original background image block; and figuring out a new background image block according to physical ratio size of the original background image block and the specified mathematical model so as to replace the original background image block. By adopting the technical scheme, the requirement on the adopted original background image block is lower, and the original background image block is not necessarily an absolute blank background image; and furthermore, the processing effect of the background image is good.

Description

technical field [0001] The invention relates to an image processing method, in particular to a background image processing method of slices under a microscope. Background technique [0002] Due to the influence of the lighting unit and the optical system, a microscope digital imaging system will always produce uneven images. Typically, the imaged digital image is brighter in the middle and darker at the sides. This image light field inhomogeneity has a great influence on the observation of imaging images, especially for digital slice scanning. In digital slice scanning, images need to be spliced. If the brightness values ​​of the left and right or top and bottom images change slightly, such as more than 5 gray levels, the spliced ​​image will have obvious brightness splicing traces. At present, there are mainly software background correction methods and hardware background correction methods for light field correction of microscope digital imaging. [0003] The software ba...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06T5/00
Inventor 肖倩陈德敏陈进
Owner MOTIC CHINA GRP CO LTD
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