Method for detecting aflatoxin by asymmetrical gold nanoparticle dimer immunosensor

A gold nanoparticle and aflatoxin technology, applied in nanotechnology, nanotechnology, nanotechnology for materials and surface science, etc., can solve problems such as blanks in the detection field

Active Publication Date: 2012-04-25
SHANDONG MEIZHENG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, the chiral research of nanoparticle assembly structure has become a hot topic of concern, but the CD signal generated by assembly is still blank in the field of detection

Method used

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  • Method for detecting aflatoxin by asymmetrical gold nanoparticle dimer immunosensor
  • Method for detecting aflatoxin by asymmetrical gold nanoparticle dimer immunosensor
  • Method for detecting aflatoxin by asymmetrical gold nanoparticle dimer immunosensor

Examples

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Embodiment 1

[0037] (1) Synthesis of 15nm gold nanoparticles

[0038] The synthesis scheme of 15nm gold nanoparticles is as follows: add 87.5mL ultrapure water to a clean three-necked flask, add 2.3mL 0.4% chloroauric acid solution, stir and heat to boiling, add 1.4mL 1% trisodium citrate after 7-8min Solution, after the solution turns from colorless to red, stop heating and continue stirring for 15 minutes; transmission electron microscope shows 15nm;

[0039] (2) Synthesis of 8nm gold nanoparticles

[0040] The synthesis scheme of 8nm gold nanoparticles is as follows: add 69mL ultrapure water and 0.9mL 1% chloroauric acid solution to a clean three-necked flask as solution A; take a clean small bottle, add 3.8mL 1% trisodium citrate solution, 0.1mL 1% tannic acid solution, 0.2mL 25mM potassium carbonate solution and 17.8mL ultrapure water were used as liquid B; both liquids A and B were heated to 60°C, and then liquid B was quickly added to liquid A under high-speed stirring, and finally...

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Abstract

The invention relates to a method for detecting aflatoxin by an asymmetrical gold nanoparticle dimer immunosensor, belonging to the technical fields of material chemistry and food safety. The method comprises the steps of: synthesis of gold nanoparticles, and circular dichroism spectrum detection for antibody / coated original coupling gold nanoparticles and the asymmetrical gold nanoparticle dimer immunosensor. The invention provides the asymmetrical gold nanoparticle dimer immunosensor for detecting the aflatoxin, so that the immunosensor capable of detecting by utilizing circular dichroism spectrum (CD) signals can be established.

Description

technical field [0001] A method for detecting aflatoxin by an asymmetric gold nanoparticle dimer immunosensor belongs to the technical fields of material chemistry and food safety. Background technique [0002] Nanoparticles (Nanoparticles, Nas) generally refer to particles with at least one dimension between 1-100 nm. The nanoscale is the transition region at the junction of atomic clusters and macroscopic objects. Materials in this region have some unique properties, such as small size effects, surface, interface effects, and quantum size effects. Although the concentration of nanoparticles in the air is very low, it has a high number of particles. After subdividing a macroscopic object into nanoparticles, its optical, thermal, electrical, magnetic, mechanical, and chemical properties will be significantly different from those of bulk solids. The small size, chemical composition, surface structure, solubility, shape and aggregation of nanomaterials determine their specia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/537G01N33/531G01N21/19
CPCB82Y40/00G01N33/54346G01N33/53B82Y30/00
Inventor 王利兵胥传来许宙
Owner SHANDONG MEIZHENG BIOTECHNOLOGY CO LTD
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