Method for detecting aflatoxin by asymmetrical gold nanoparticle dimer immunosensor
A gold nanoparticle and aflatoxin technology, applied in nanotechnology, nanotechnology, nanotechnology for materials and surface science, etc., can solve problems such as blanks in the detection field
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[0037] (1) Synthesis of 15nm gold nanoparticles
[0038] The synthesis scheme of 15nm gold nanoparticles is as follows: add 87.5mL ultrapure water to a clean three-necked flask, add 2.3mL 0.4% chloroauric acid solution, stir and heat to boiling, add 1.4mL 1% trisodium citrate after 7-8min Solution, after the solution turns from colorless to red, stop heating and continue stirring for 15 minutes; transmission electron microscope shows 15nm;
[0039] (2) Synthesis of 8nm gold nanoparticles
[0040] The synthesis scheme of 8nm gold nanoparticles is as follows: add 69mL ultrapure water and 0.9mL 1% chloroauric acid solution to a clean three-necked flask as solution A; take a clean small bottle, add 3.8mL 1% trisodium citrate solution, 0.1mL 1% tannic acid solution, 0.2mL 25mM potassium carbonate solution and 17.8mL ultrapure water were used as liquid B; both liquids A and B were heated to 60°C, and then liquid B was quickly added to liquid A under high-speed stirring, and finally...
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