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Technology for propagating watermifoil through hormone induction

A technology of foxtail algae and hormones, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of affecting the speed of breeding foxtail algae seedlings, the decrease of survival rate, and the complicated operation of tissue culture.

Inactive Publication Date: 2012-05-16
南京中科水治理股份有限公司
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AI Technical Summary

Problems solved by technology

[0003] At present, the methods of propagating Foxtail algae mainly include cutting propagation and tissue culture. The operation of tissue culture is relatively complicated and the conditions are strict.
The operation of cutting propagation is relatively simple, but the survival rate of explants will be greatly reduced when the explants are below 10 cm, which seriously affects the propagation speed of foxtail algae seedlings

Method used

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Embodiment Construction

[0029] The method for cultivating foxtail algae seedlings by hormone induction, the specific implementation measures are as follows:

[0030] 1. Collect plants that are in good growth condition and free from diseases and insect pests.

[0031] 2. After collection, wash with clean water 3 times, and remove the impurities in it.

[0032] 3. Cut the explants into 5-8 cm long, and remove the leaves.

[0033] 4. Preparation of Hoagland culture medium. Hoagland culture medium includes: Hoagland basic elements, indole butyric acid 2.5mg / L, gibberellin 0.2mg / L.

[0034] 5. Put the cut explants into Hoagland medium for culture. Under the conditions of a room temperature of 25° C. and a day-to-night ratio of 1:1, they were cultured for 12 days.

[0035] 6. When the new root system grows to 2 cm and the lateral buds grow to 5 cm, take it out of the culture medium and rinse it once with clean water. Planted in nursery fields for cultivation.

[0036] 7. Keep the water depth at 30 cm...

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Abstract

The invention discloses a method for propagating watermifoil and belongs to the biotechnology field. The method comprises the following operation steps: (1) cutting the explant of the watermifoil to 5-8cm; (2) culturing the explant in a Hoagland culture solution, inducing the root to generate under the condition of adding 0.5-5mg / L of indolebutyric acid, and inducing a lateral bud to grow under the condition of adding 0.1-0.5mg / L of gibberellin; and (3) culturing for 10-15 days to grow a completed plant, wherein the temperature of a culture room is 25DEG C and the ratio of day to night is 1:1. The completed plant is taken out of the culture solution, and is cultured in a seedling growing field continually, and the survival rate can be over 95% and is obviously improved compared with that of the watermifoil explant through direct cuttage.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a technology for hormone-induced propagation of submerged plant Pleurotus chinensis. Background technique [0002] Myriophyllum spicatum L., Myriophyllum spicatum L., Haloragidaceae (Myriophyllum), a perennial submerged plant. It often forms various types of aquatic plant communities with bitter grass and hornwort. In the natural restoration of aquatic vegetation, foxtail algae is also one of the first pioneer species to survive and settle. Due to the strong pollution resistance of foxtail algae, it is the priority planted aquatic plants in the aquatic vegetation restoration project. [0003] At present, the main methods of propagating Trichosanthes include cutting propagation and tissue culture, and the operation of tissue culture is relatively complicated and the conditions are strict. The operation of cutting propagation is relatively simple, but the surviva...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/00
Inventor 柴夏刘平平史加达呙明杰刘从玉万陆军陈小峰谢超
Owner 南京中科水治理股份有限公司