Low molecular heparin iron nanoparticles and preparation method thereof

A low-molecular-weight heparin and nanoparticle technology, which is applied in the field of preparation of low-molecular-weight heparin iron nanoparticles, can solve problems such as medication inconvenience, and achieve the effect of reducing the frequency of administration and reducing the administration time

Active Publication Date: 2012-05-30
吴忠仕 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Heparin and LMWH, which are commonly used in clinical practice, need to be administered multiple times a day, which brings inconvenience to inpatients and non-hospitalized patients. If the drug effect can last for several days with one administration, it will bring huge economic and social effects

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  • Low molecular heparin iron nanoparticles and preparation method thereof
  • Low molecular heparin iron nanoparticles and preparation method thereof
  • Low molecular heparin iron nanoparticles and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Preparation and detection of low molecular weight heparin iron nanoparticles

[0031] Method: (1) Preparation of dihydroxyl (Ⅲ) iron ion (DHOF) solution:

[0032] The mass concentration of DHOF solution is 1-20g / L, among which 5-10g / L is more suitable. FeCl with deionized water 3 ·6H 2 Dissolve O to twice the required concentration; weigh the amount of NaOH according to the molar number of Fe:OH=1:2, and dissolve it with the same volume of deionized water. Under the condition of magnetic stirring, NaOH solution was slowly dropped into FeCl 3 In the solution, a dihydroxyl (Ⅲ) iron ion solution with a pH value of 3.0 is prepared.

[0033] (2) Preparation of low molecular weight heparin (abbreviated as LMWH) solution:

[0034] The mass concentration of LMWH is 1-20g / L, among which 5-10g / L is more suitable. And add sodium chloride to the LMWH solution, the concentration of sodium chloride is 1-5g / L.

[0035] (3) Preparation of low molecular weight heparin iron nanopa...

Embodiment 2

[0046] Infrared Absorption Spectrum of Low Molecular Weight Heparin Iron Nanoparticles

[0047] Method: The mass ratio of DHOF to LMWH is 1.0, under the condition of ultrasonic vibration, the prepared low molecular weight heparin nanoparticle solution (the production method is the same as that in Example 1), and the prepared low molecular weight heparin nanoparticle solution is placed in vacuum at -54°C After drying, mix 10 mg of low-molecular-weight heparin iron nanoparticles powder and LMWH powder with 90 mg of potassium bromide powder and press them into tablets, and then use a Fourier transform infrared instrument to measure low-molecular-weight heparin iron nanoparticles and LMWH infrared. absorption spectrum.

[0048] Results: The infrared absorption spectra of low-molecular-weight heparin iron nanoparticles and LMWH are generally similar (Fourier transform infrared absorption spectra are shown in the appendix Figure 4 ). Due to [Fe(OH) 2 ] + Interacting with LMWH, ...

Embodiment 3

[0051] In vitro sustained release detection of low molecular weight heparin iron nanoparticles

[0052] Method: the prepared low-molecular-weight heparin nanoparticle solution (manufacturing method is the same as in Example 1), the nanoparticle solution was subjected to high-speed centrifugation at 10,000 rpm for 20 minutes, the supernatant was discarded, the precipitate was washed 3 times with deionized water, and 10 mg was weighed after drying. Use 10ml of PBS solution to oscillate evenly, then ultrasonically disperse evenly, put it in a dialysis bag with a molecular weight of 12000, put it into 190ml of PBS solution, shake at 80rpm, take 100ul samples from PBS on 1d, 2d, 1w, 2w, and 4w respectively, and detect its concentration of LMWH.

[0053] Results: The release of low-molecular-weight heparin nanoparticles was relatively large on the first day, accounting for about 5.1% of the total amount, followed by uniform release, and the release amount accounted for about 43.8% o...

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Abstract

The present invention relates to a low molecular heparin iron nanoparticle biological product. The invention further relates to a preparation method for the low molecular heparin iron nanoparticles. According to the present invention, the preparation method for the low molecular heparin iron nanoparticles with excellent slow release function is provided; with the method, the low molecular heparin iron nanoparticles capable of slow release of LMWH in vivo can be obtained, and the heparin administration time and the times of the heparin administration can be effectively reduced with the drug.

Description

technical field [0001] The invention relates to a low molecular weight heparin iron nano particle biological product. [0002] The invention also relates to a preparation method of the low-molecular-weight heparin iron nanoparticles. technical background [0003] Heparin is a type of glycosaminoglycan, a mixture of polysaccharide chains consisting of repeating disaccharide units linked by uronic acid and glucosamine with 1→4 glycosidic bonds. Heparin effectively inhibits thrombin by binding antithrombin III (AT-III), thereby delaying blood coagulation, preventing thrombosis, and promoting thrombus dissolution. [0004] The molecular weight of heparin is about 3000-15000, with an average of 16000. The molecular weight of low molecular weight heparin (LMWH) is lower than 6000-10000. LMWH has the effect of selective anticoagulation factor Xa activity, and has no obvious effect on thrombin and other coagulation factors. The anticoagulant effect of unfractionated heparin is ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K9/14A61K47/48A61K31/727A61P7/02
Inventor 陶运明吴忠仕
Owner 吴忠仕
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