Enhancer Hr3

Abstract

The invention relates to enhancer Hr3 adopting the nucleotide sequence shown in the SEQIDNO: 1. The enhancer Hr3 can be used for preparing and recombining heterologous protein by bombyx mori. The invention further relates to a recombination carrier containing the enhancer Hr3, and the recombination carrier is prepared in such a manner that the enhancer Hr3 is connected with a Nco1 restriction site at the upstream of a promoter of a carrier containing no enhancer through the Nco1 restriction site of the enhancer Hr3. In the invention, functional elements such as the first grade enhancer, activating transcription factor IE1, untranslated region sequences (5' UTR and 3' UTR) and the like which are expressed by intensifier are identified in the cellular level, and an efficient and stable sericin I expression system is built by integrating optimum elements on the basis, so that efficient expression recombination heterologous protein can be made of middle silk gland of transgenic bombyx mori.

Description

technical field [0001] The invention relates to biological genetic engineering, in particular to the field of transgenic silkworm. Background technique [0002] With the rapid development of bioengineering and genetic engineering in the 21st century, people have an increasing demand for functional proteins for various purposes such as medical, medicinal, edible, beauty, and health care, which cannot be met by relying on protein extraction and production from natural sources. Rapidly growing market demand. Establishing and improving various high-efficiency prokaryotic and eukaryotic expression systems, and using strains, cells, and insects as host bioreactors is an effective and sustainable way to achieve low-cost, large-scale production of recombinant foreign proteins with biological activity. method, and has become a research hotspot in the world today. Using Chinese hamster ovary cells as a bioreactor to produce foreign proteins is currently the most standard expression ...

AI Technical Summary

Problems solved by technology

[0005] The expression of foreign genes in eukaryotic cells and tissues is regulated by a series of regulation such as transcription level, post-transcriptional regulation (including mRNA transport and stability regulation), translation efficiency, post-translational regulation, and protein secretion. The regulatory region, the cis-acting elements in the ORF of the foreign gene and the trans-regulatory factors in the host cooperate to complete the process, which is extremely precise and complex

On the other hand, pBac-based transposon-mediated silkworm transgenesis is the insertion and integration of exogenous genes carried by pBac into the silkworm genome in a random manner. This random insertion often makes the exogenous genes affected by the position effect of the insertion site in the genome. , so that the expression of exogenous genes decreased

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