Detection method for cytotoxicity of gas-liquid contact type cigarettes under full-smoke exposure

A technology of cytotoxicity and gas-liquid contact, applied in measuring devices, air quality improvement, instruments, etc., can solve the problems of undiscovered public reports, etc., and achieve the effect of low cost

Active Publication Date: 2014-08-13
YUNNAN RES INST OF TOBACCO SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But through document retrieval, do not find the public report of identical document with the content of the present invention

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  • Detection method for cytotoxicity of gas-liquid contact type cigarettes under full-smoke exposure
  • Detection method for cytotoxicity of gas-liquid contact type cigarettes under full-smoke exposure
  • Detection method for cytotoxicity of gas-liquid contact type cigarettes under full-smoke exposure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Detection of neutrophil toxicity under exposure to full smoke of a certain mixed type of cigarette

[0034] Prepare the CHO cell suspension according to the above step a., and then follow the above steps b., c. and d. to expose the CHO cells to cigarette smoke. When the cigarette sample is smoked, the mainstream smoke of the cigarette is diluted with clean air and then introduced into ZL201120063251 .6 In the full-smoke exposure bottle, set 4 different clean air flow rates (0mL / min, 250mL / min, 500mL / min, 750mL / min, 1000mL / min) to dilute the mainstream cigarette smoke. The exposure time is 5 minutes, the smoking volume of the cigarette is 35mL, the duration of smoking is 2s, and the interval between smoking is 58s.

[0035] After the smoke exposure is complete, the neutrophil toxicity test is carried out according to the above step e. (1), and the cell inhibition rate CI is calculated according to the absorbance value.

[0036] CI = ...

Embodiment 2

[0045] Example 2: MTT cytotoxicity detection under full smoke exposure of a certain mixed type of cigarette

[0046] Except for performing the MTT cytotoxicity test according to step e.(2), other experimental methods are the same as in Example 1. See Table 3 and Table 4 for the test results of MTT cytotoxicity under full smoke exposure of a certain mixed cigarette, and analyze the dose-response relationship between smoke exposure and cytotoxicity in the MTT cytotoxicity test under full smoke exposure, see figure 2 .

[0047] From Table 3 and figure 2 It can be seen that as the flow rate of clean air increases, the cell inhibition rate produced by smoke decreases, showing a significant dose-response relationship.

[0048] Table 3 MTT cytotoxicity test results of a mixed cigarette exposed to full smoke

[0049]

[0050]

[0051] Table 4 MTT cytotoxicity detection in smoke dilution multiples and cell inhibition rate

[0052]

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Abstract

The invention relates to a detection method for the cytotoxicity of gas-liquid contact type cigarettes under full-smoke exposure, belonging to the technical field of safety evaluation of cigarette smoke. The detection method provided by the invention comprises the following steps of: firstly, setting cigarette suction parameters to carry out suction on a cigarette; diluting the smoke by clean air with the flow speed of 0-1000 mL / min and transferring the diluted cigarette full smoke into a full-smoke exposure bottle disclosed in the patent with the patent number of ZL201120063251.6 to come into contact and exposure with suspension cells in the bottle, wherein the exposure time is 5 min; after the exposure, carrying out neutral erythrocyte toxicity test or MTT (Methyl Thiazolyl Tetrazolium) cytotoxicity test according to a regular method; analyzing a dosage reaction relation between smoke exposure amount and cytotoxicity according to the following formula: full-smoke dilution multiple=( flow speed of smoke + flow speed of clean air) / flow speed of smoke; smoke exposure amount under exposure of 10 mL of a cell suspension solution for 5 min=TPM (Total Particulate Matter) amount of single cigarette / suction times of single cigarette*6 suctions / 10 mL; and representing the extent of cytotoxicity by a cell inhibition ratio CI. The detection method provided by the invention has the advantages of simplicity, feasibility, low cost and the capability of providing technical supports for the safety evaluation of cigarette smoke.

Description

Technical field: [0001] The invention relates to a method for detecting cytotoxicity under gas-liquid contact type cigarette smoke exposure, and belongs to the technical field of cigarette smoke safety evaluation. Background technique: [0002] The research on the whole smoke exposure method for tobacco toxicological evaluation has attracted worldwide attention because of its irreplaceable advantages in simulating the environment in the human body, realizing multiple relevant detection endpoints, and online real-time detection of original full smoke. Tobacco companies and analysis and testing laboratories are widely concerned, and some tobacco companies have conducted in-depth and systematic research on this exposure method. [0003] At present, there are patent publications related to the method of exposing the whole cigarette smoke to the outside. Publication Patent 1 (Application No.: 200810230659.0) is a cytotoxicity test method of cigarette mainstream smoke. The main e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/00
CPCY02A50/20
Inventor 米其利夭建华管莹李雪梅周岚缪明明
Owner YUNNAN RES INST OF TOBACCO SCI
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