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Analysis and assay of glycated hemoglobins by capillary electrophoresis, buffer compositions, and kits for capillary electrophoresis

A technology of glycosylated hemoglobin and capillary electrophoresis, which is applied in the field of analysis and determination of glycosylated hemoglobin to achieve the effect of reliable reading

Active Publication Date: 2014-11-05
SEBIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From a practical point of view, this double coating must be reapplied between each analysis using the exact procedure provided by the kit manufacturer, but this procedure is only for single capillary devices ((P / ACE, Beckman), not suitable for routine analysis) elaboration

Method used

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  • Analysis and assay of glycated hemoglobins by capillary electrophoresis, buffer compositions, and kits for capillary electrophoresis
  • Analysis and assay of glycated hemoglobins by capillary electrophoresis, buffer compositions, and kits for capillary electrophoresis
  • Analysis and assay of glycated hemoglobins by capillary electrophoresis, buffer compositions, and kits for capillary electrophoresis

Examples

Experimental program
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Effect test

Embodiment 1

[0228] Normal human blood (containing hemoglobin HbA) diluted to 1 / 6 in hemolysis solution (1 g / L TritonX100 dissolved in 0 , HbA 1 and HbA 2 ) Capillary electrophoresis was performed using the assay buffer described in patent US5,599,433, which contains 100 mM CAPS and 300 mM orthoboric acid at a pH of 11. The obtained electropherograms are shown in figure 1 middle. The separation between the peaks for hemoglobin is less pronounced.

Embodiment 2

[0230] From different purified fractions containing glycosylated hemoglobin (fraction A 0 and A 1c or Part A 0 、A 1b and A 1a ) reference sample (Exocell, USA) was subjected to capillary electrophoresis using the analytical buffer described in the patent US5,599,433, which contained 100 mM CAPS and 300 mM orthoboric acid, pH 10.20. Income A 1a 、A 1b and A 1c The standard electrophoretic curve is shown in figure 2 middle. HbA 1c with HbA 1a 、A 1b Significantly insufficient separation between hemoglobins; HbA 1c Electrophoretic peak covering HbA1 a / HbA1 b peak.

Embodiment 3

[0232] From different purified fractions containing glycosylated hemoglobin (fraction A 0 and A 1c or Part A 0 、A 1b and A 1a ) reference sample (Exocell, USA) was subjected to capillary electrophoresis using a buffer composition containing 200 mM CAPSO and 10 mM putrescine (pH 10.20), but neither an orthoborate compound nor a borate-based compound . Income A 1a 、A 1b and A 1c The standard electrophoretic curve is shown in image 3 middle. Corresponds to HbA1 c peak of HbA o peaks co-migrate.

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Abstract

The invention relates to a method for analysis by capillary electrophoresis of glycated haemoglobins comprising at least one globin chain comprising a glucose residue bound to the amino acid in the N-terminal position, contained in a biological sample, said method comprising using a buffer composition comprising at least one compound which is capable of specifically complexing glucose residues of one or several glycated haemoglobin(s) and of providing said glycated haemoglobin(s) with several negative electric charges at an alkaline pH. By way of example, this compound may be 3,4- or 3,5-dicarboxyphenylboronic acid, preferably 3,5-dicarboxyphenylboronic acid. Said method may in particular be used to separate and assay haemoglobin HbA1c present in a biological sample optionally comprising other haemoglobins, in particular other minor fractions. The invention also concerns buffer compositions for use in said analysis, as well as kits for the analysis and for the assay of glycated haemoglobins by capillary electrophoresis.

Description

technical field [0001] This application relates to the determination of glycated hemoglobin (specifically hemoglobin A) by capillary electrophoresis 1c ) analysis and measurement field. [0002] The present invention relates to a method of analysis by capillary electrophoresis of a biological sample comprising one or several hemoglobins and in particular one or several glycated hemoglobins, and to a suitable buffer for this analysis A liquid composition, and a kit for analysis and measurement of glycated hemoglobin by capillary electrophoresis. Background technique [0003] Hemoglobin (Hb) is a spherical molecule generally comprising four subunits, each composed of a polypeptide chain bound to a hemoglobin portion. Polypeptide chains are loosely named by the name "globin portion" of the hemoglobin molecule. [0004] All human hemoglobin consists of four polypeptide chains, divided into two groups of two identical chains. In adult humans, three types of hemoglobin are com...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447
CPCG01N27/44747G01N27/447G01N33/72G01N27/44791G01N33/723
Inventor G.德沙姆普斯F.罗伯特D.西莫宁
Owner SEBIA
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