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Composite probiotic microorganism feed additive, preparation method and application thereof

A technology of microbial feed and compound probiotics, which is applied in the field of compound microbial feed additives, can solve the problems of restricting the scale of probiotic products, single function of bacteria, and low number of viable bacteria, so as to improve animal growth performance, reduce bait coefficient, The effect of reducing the material-to-weight ratio

Inactive Publication Date: 2012-06-27
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual industrial production, the number of viable bacteria is low, the function of the bacteria is single, and the stability is poor and strongly restricts the scale and industrialization of probiotic products.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Clostridium butyricum in the form of freeze-dried powder (purchased from China Industrial Microorganism Culture Collection Management Center, preservation number 20036), Bacillus natto (purchased from China Agricultural Microbiology Culture Collection Management Center, preservation number ATCC10619), lactic acid Bacillus (purchased from China Agricultural Microbiology Culture Collection Management Center, preservation number ATCC10637), Bacillus coagulans (purchased from China Industrial Microbiology Culture Collection Management Center, preservation number 10067), Bifidobacterium as probiotics (purchased from China Industrial Culture Preservation Management Center, preservation number 6169), mixed at a mass ratio of 1:7:1:2:1. Xylo-oligosaccharides, fructo-oligosaccharides and malto-oligosaccharides are selected as prebiotics and mixed in a mass ratio of 3:1:2. Microcrystalline kaolinite, shell powder, sweet potato starch, and corn starch are selected as carriers and ...

Embodiment 2

[0025] Clostridium butyricum, Bacillus natto, Lactobacillus, Bacillus coagulans and Bifidobacterium in the form of freeze-dried powder are selected as probiotics and mixed in a mass ratio of 2:10:2:4:3. Xylooligosaccharides, fructooligosaccharides and maltooligosaccharides are selected as prebiotics and mixed in a mass ratio of 6:2:4. Microcrystalline kaolinite, shell powder, sweet potato starch, and corn starch are selected as carriers and mixed in a mass ratio of 1:1:2:2. Add probiotics, prebiotics, and carriers into a mixer at a mass ratio of 3:10:135, stir for 8 minutes at 250 rpm and 10°C, and mix evenly to prepare a compound probiotic microbial feed additive.

[0026] Put the prepared compound probiotic microbial feed additive into common aquatic feed at 1% by mass; put into common full-price pig feed at 4% by mass.

[0027]

Embodiment 3

[0029] Penaeus vannamei was selected as the test object, and the test was divided into two groups, wherein 1wt% of this additive was added to the feed of one group, and the control group did not add this additive. The test results are recorded as follows:

[0030] Additive feed group Feed group without additives Pond area (mu) 20 20 The total number of shrimp seedlings (only) 20000 20000 Experiment cycle 8 8 Initial weight (g) 0.01±0.001 0.01±0.001 Weight gain (%) 2599.1±388.8 2332.1±209.5 Survival rate (%) 80.1%±1.2 77.6±1.3 Specific growth rate (%) 6.01±0.21 5.69±0.19 Feed to meat ratio 2.13±0.13 2.26±0.15 Protein efficiency (%) 1.11±0.11 0.99±0.09

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Abstract

The invention discloses a composite probiotic microorganism feed additive, a preparation method and application thereof. The composite probiotic micro-organism feed additive is characterized by comprising probiotics, prebiotics and vectors. The preparation method of the composite probiotic microorganism feed additive comprises the following step of uniformly mixing the probiotics, the prebiotics and the vectors. When the composite probiotic microorganism feed additive is applied to aquatic feeds, the mass percentage of the addition amount of the composite probiotic micro-organism feed additive is 0.8-1%; and when the composite probiotic microorganism feed additive is applied to complete pig feeds, the mass percentage of the addition amount of the composite probiotic microorganism feed additive is 2-4%. The composite probiotic microorganism feed additive disclosed by the invention has the advantages of improving growth performance of animals, reducing bait material coefficients, increasing appetite of the animals and improving immunity function of the animals, and realizes a purpose of improving meat quality of the animals.

Description

technical field [0001] The invention relates to the field of feed additives, in particular to a compound microbial feed additive containing compound probiotics, prebiotics and carriers. Background technique [0002] Antibiotics play a role in disease prevention, improvement of bait coefficient, etc. in the process of breeding and promoting growth, and improve breeding efficiency. However, the long-term abuse of antibiotics has brought many adverse effects, such as bacterial resistance, superbug formation, DNA pollution, etc., which affect the micro-ecological balance and body immunity of animals, and through the food chain, directly affect human health and increase the risk of human injury. [0003] At present, many countries, such as South Korea, Japan, and the European Union, have banned the artificial addition of antibiotics in the breeding process, and my country has also begun to establish relevant laws and regulations. The world's breeding industry has gradually entere...

Claims

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Application Information

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IPC IPC(8): A23K1/16
Inventor 江瀚马正驰
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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