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Method for extracting flavonoid compound from marigold residue

A technology of flavonoids, marigold, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve problems such as resource waste, environmental impact, etc., achieve low equipment and production costs, strong practicability characteristics, The effect of the simple process route

Inactive Publication Date: 2012-06-27
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This has a certain impact on the environment, but also causes a lot of waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the extraction of flavonoids

[0025] (1) Prepare the marigold residue raw material, and crush it through a 20-mesh sieve;

[0026] (2) Add 100 g of marigold residues with mixed particle sizes into the acetone aqueous solution with a mass percentage of acetone of 40% according to the mass volume ratio of 1:10 (g / ml), and place in a constant temperature water bath shaker at 40°C (100r / min) extract 40min, suction filtration obtains the crude extract of flavonoids;

[0027] (3) Concentrate the crude extract 5 times to obtain a concentrated solution; place the concentrated solution at 4°C for 2 hours, remove the supernatant, and dry to obtain 6.27 g of the crude extract;

[0028] (4) Repeat the above steps to prepare 30 g of crude extract, add it into n-hexane according to the mass volume ratio of 1:9 (g / ml), and elute 1 times, the crude extract 29.04g of fat-soluble substances was removed;

[0029] (5) Add the crude extract obtained from the removal of fat...

Embodiment 2

[0030] Embodiment 2, the extraction of flavonoids

[0031] (1) Prepare the marigold residue raw material, and crush it through a 40-mesh sieve;

[0032] (2) The marigold residue of 100g mixed particle size is added in the acetone aqueous solution that the mass percent content of acetone is 60% according to the mass volume ratio is 1: 20 (g / ml), in the constant temperature water bath shaker at 50 ℃ (100r / min) extraction 80min, suction filtration obtains the crude extract of flavonoids;

[0033] (3) Concentrate the crude extract 5 times to obtain a concentrated solution; place the concentrated solution at 4°C for 2 hours, remove the supernatant, and dry to obtain 7.28 g of the crude extract;

[0034] (4) Repeat the above steps to prepare 30 g of crude extract, add it to n-hexane according to the mass volume ratio of 1:12 (g / ml), and elute in a constant temperature water bath shaker (100 r / min) at 50 ° C for 40 min. times, obtain the crude extract 28.52g of removing fat-soluble...

Embodiment 3

[0036] Embodiment 3, the extraction of flavonoids

[0037] (1) Prepare the marigold residue raw material, and crush it through a 60-mesh sieve;

[0038] (2) Add 100 g of marigold residues with mixed particle sizes into the acetone aqueous solution with a mass percent content of 70% of acetone according to the mass volume ratio of 1:30 (g / ml), and place in a constant temperature water bath shaker at 60 ° C. (100r / min) extract 120min, suction filtration obtains the crude extract of flavonoids;

[0039] (3) Concentrate the crude extract 5 times to obtain a concentrated solution; place the concentrated solution at 4°C to cool for 2 hours, remove the supernatant, and dry to obtain 7.85 g of the crude extract;

[0040](4) Repeat the above steps to prepare 30 g of crude extract, add it to n-hexane according to the mass volume ratio of 1:15 (g / ml), and elute it in a constant temperature water bath shaker (100 r / min) at 60 ° C for 50 min. times, obtain the crude extract 28.23g of rem...

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Abstract

The invention discloses a method for extracting a flavonoid compound from a marigold residue. The method comprises the steps of: (1) conducting extraction on the marigold residue with an acetone water solution so as to obtain a crude extraction solution; (2) concentrating the crude extraction solution so as to obtain a concentrated solution, which is then cooled to obtain a crude extract; and (3) eluting the crude extract with n-hexane so as to obtain a fat-soluble impurity removed crude extract, which is then eluted by a sodium hydrogen phosphate-citric acid buffer solution, thus obtaining a product. The method of the invention takes a marigold residue as the raw material, which is subjected to crushing, sieving, as well as rapid and effective extraction of a flavonoid substance by a water solution of the organic solvent. And through concentration, precipitation, drying, and purification, an obtained yellow substance has a flavonoid compound content of over 90g RE / 100g. The method provided in the invention has the advantages of reasonable technical parameters, simple technological path, simple and safe operation, high yield, low equipment and production cost, and great practical value as well as social and economic benefits.

Description

technical field [0001] The invention relates to a method for extracting flavonoids from marigold residue. Background technique [0002] Marigold is one of the main herbal potted flowers cultivated in my country, and it is widely used in indoor and outdoor environment layout. With the development of the nutrition and health industry, the international demand for marigolds is increasing. Marigold not only has ornamental value, but also has good medicinal value. The main components of its pharmacological effects are flavonoids, carotenoids, terpenes and essential oils. Tagetes and its congeners contain common flavonoids such as quercetin, kaempferol, and myricetin, and more flavonoids exist in the form of glycosides, such as tagetes, quercetin tagetes, and kaempferols. Flavonoids have the antioxidant activity of scavenging free radicals, strong anti-tumor effect, anti-cancer effect, lowering blood fat, lowering blood sugar, and preventing cardiovascular and cerebrovascular di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08C07D311/30C07D311/40
CPCY02P20/54
Inventor 高彦祥蒋俊平袁芳
Owner CHINA AGRI UNIV
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