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Application of cuphea hossipifolia kunth extractives in cosmetics and medicines

A technology of Calyx chinensis and its extract, which is applied in the field of cosmetics and medical supplies, and can solve the problems of moisturizing, whitening and anti-oxidation that have not been seen

Inactive Publication Date: 2012-07-04
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report in the prior art that the extract obtained from the plant Cuphea hossipifolia Kunth has moisturizing, whitening and antioxidant effects on the skin.

Method used

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  • Application of cuphea hossipifolia kunth extractives in cosmetics and medicines
  • Application of cuphea hossipifolia kunth extractives in cosmetics and medicines
  • Application of cuphea hossipifolia kunth extractives in cosmetics and medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Plant material and extraction method:

[0045] 4.4 kg Cuphea hossipifolia Kunth dried in the sun, crushed, soaked in ethanol three to four times at room temperature, filtered, and the ethanol solution was concentrated under reduced pressure to obtain an extract (292.5 grams). After adding an appropriate amount of water to 20 g of the extract, extract with ethyl acetate to obtain ethyl acetate (9.23 g) and water components. The aqueous layer was passed through a D101 macroporous resin column, and the column was eluted with water, 25% methanol-water, 50% methanol-water, 75% methanol-water and methanol to obtain different fractions, water (11.68 grams), 25% methanol - water (0.32 g), 50% methanol-water (0.18 g), 75% methanol-water (0.34 g) and methanol (0.09 g) (see figure 1 ). The silica gel column chromatography of the ethyl acetate fraction was eluted with petroleum ether, 25% acetone-petroleum ether, 50% acetone-petroleum ether, 75% acetone-petroleum ether, acetone a...

Embodiment 2

[0064] Toxic activity test on B16 cells:

[0065]On a 96-well cell culture plate, mix B16 cells with different concentrations of the drug solution to be tested, set up 8 replicate wells, and set a blank control without drug at the same time, 37 ° C, 5% CO 2 After culturing for 24 hours, the cytotoxicity was detected by the MTS colorimetric method, and the OD value was measured by a microplate reader at a wavelength of 490 nm. Calculated to get CC 50 The value (50% cytotoxic concentration) is the concentration of the drug that produces toxicity to 50% of B16 cells.

[0066] Table 1 The results of the toxic effect of samples on B16 cells

[0067]

[0068]

[0069]

Embodiment 3

[0071] In vitro antioxidant activity test:

[0072] Mix and react the drug to be tested with DPPH (final concentration: 100 μM), set up 3 replicate wells, and set a blank control without drug and Trolox positive control at the same time, at 30°C for 1 h, measure the OD value with a microplate reader, and the detection wavelength is 515nm. Calculate the antioxidant rate.

[0073] Table 2 In vitro antioxidant activity results of samples

[0074]

[0075]

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PUM

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Abstract

The invention discloses a cosmetic ingredient with cuphea hossipifolia kunth extractives as raw materials for skin whitening, moisturizing and antioxidant activity and combination application in cosmetics. The cuphea hossipifolia kunth extractives are produced by drying cuphea hossipifolia kunth in the sun, smashing the cuphea hossipifolia kunth, soaking three times to four times at the room temperature, filtering, decompressing with ethnol liquid, concentrating into extract, adding defined amount of water, and extracting sequentially with petroleum ether and ethyl acetate to respectively obtain petroleum ether and ethyl acetate. A water layer flows through a D101 large hole resin column which is eluted by 25% methanol-water, 50% methanol-water, 75% methanol-water and methanol to obtain different distilling sections, the ethyl acetate is subjected with silica column chromatography to be eluted successively with petroleum ether, 25% acetone-petroleum ether, 50% acetone-petroleum ether, 75% acetone-petroleum ether, acetone and methanol to achieve different distilling sections, or ethanol of different concentration is used for extracting to obtain different extracts.

Description

Field of invention: [0001] The invention belongs to the field of cosmetics and pharmaceuticals, in particular, it relates to a cosmetic composed of an active ingredient of Cuphea hossipifolia Kunth extract, and its application in the preparation of cosmetics, and its use in the preparation of drugs for the prevention or treatment of skin diseases Application, in the preparation of anesthetics, antifungal agents, anti-inflammatory agents, antioxidants, preservatives, chelating agents, coloring agents, decolorizing agents, softeners, emulsifiers, exfoliating agents, film-forming agents, fragrances, wetting agents, insect repellants Agents, lubricants, moisturizers, pharmaceuticals, photostabilizers, preservatives, skin protectants, skin penetration enhancers, sunscreens, stabilizers, surfactants, thickeners, viscosity regulators, vitamins, Background technique: [0002] Aging, long-term exposure to unfavorable environments, malnutrition, and fatigue can all cause changes in sk...

Claims

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Application Information

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IPC IPC(8): A61K8/97A61K36/185A61P17/00A61P17/18A61P17/04A61P29/00A61Q19/02A61Q19/08A61Q19/00A61Q5/12
Inventor 刘吉开丁智慧白雪贾锐锐瞿燕董泽军
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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