Chaenomeles speciosa extract and cosmetic by taking extract as active ingredient

A wrinkled papaya extract technology, applied in the field of wrinkled papaya extract and cosmetics using it as an active ingredient, to achieve the effect of improving utilization function and added value, good safety, and improving resource utilization value

Inactive Publication Date: 2016-02-03
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has been no report that the composition of wrin...

Method used

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  • Chaenomeles speciosa extract and cosmetic by taking extract as active ingredient
  • Chaenomeles speciosa extract and cosmetic by taking extract as active ingredient
  • Chaenomeles speciosa extract and cosmetic by taking extract as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of wrinkled papaya extract:

[0029] Weigh 16 g of chaenomeles speciosa (Sweet) Nakai) seeds, grind them into powder, and use 25 times (482 mL) of 85% ethanol for cold soaking for 24 hours, and then collect the filtrate with a Buchner funnel and repeat 3 times. The filtrate was distilled under reduced pressure with a rotary evaporator, the filtrate was concentrated, and the concentrated filtrate was dried at low temperature to obtain a crude extract. The crude extract was adsorbed and decolorized by macroporous resin (D101), and eluted with 95% ethanol, the eluate was concentrated and evaporated to dryness, and freeze-dried at low temperature to obtain 1 g of papaya seed extract with an extraction rate of 6.25%.

[0030] Alternatively, weigh 16 g of chaenomeles speciosa (Sweet) Nakai) seeds, grind them into powder, and cold-soak them with 20 times the amount of 95% ethanol for 20 hours, and then collect the filtrate with a Buchner funnel and repeat 4 times. The ...

Embodiment 2

[0032] Cytotoxicity test of wrinkled papaya extract: Determine the CC of the sample by MTS colorimetry 50 (50%cytotoxicconcentration), that is, the concentration of the drug when it is toxic to 50% of the cells, so as to determine the safe sample concentration for the following activity experiment.

[0033] Experimental method: On a 96-well cell culture plate, mix B16 cells with different concentrations of the drug solution to be tested, set 3 replicate wells, and set a blank control without drug at 37°C, 5% CO 2 Cultivate for 24h, use MTS colorimetric method to detect cytotoxicity, measure OD value with microplate reader, and measure wavelength at 490nm. Calculate CC 50 value.

[0034] Cell survival rate (%) = OD of experimental well 490nm / Blank hole OD 490nm ×100%

[0035] Experimental results: The cell survival rate of the papaya seed extract is 96.901%, which is a normal level. It can be clearly seen that the papaya extract has no toxic effect on cells, and its safety as a nat...

Embodiment 3

[0037] DPPH free radical scavenging experiment: mix and react the drug to be tested with DPPH (final concentration 100μM), set 3 repetitive wells, set a blank control without drug and a Trolox positive control at the same time, 30℃, 1h, microplate reader measurement OD value, detection wavelength is 515nm. Calculate the antioxidant rate.

[0038] Anti-oxidation rate (%) = (1-test hole OD 515nm / Blank hole OD 515nm )×100%

[0039] Experimental results: In the experiment, the extract of papaya seed showed significant DPPH free radical scavenging ability, and according to the concentration gradient, it showed a good dose-effect relationship (see figure 1 ), can be used as a good natural plant source with anti-wrinkle or sunscreen potential cosmetic activity.

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Abstract

The invention discloses a chaenomeles speciosa (Chaenomeles speciosa (Sweet) Nakai) plant extract, a cosmetic additive with effects of whitening skin, moisturizing and achieving antioxidant activity, as well as a combined application thereof in cosmetics. The chaenomeles speciosa extract refers to an ethanol extract of chaenomeles speciosa seeds, and the extract has good capability of scavenging radicals DPPH and is a good natural antioxidant. In the human skin protection aspect, the chaenomeles speciosa extract has excellent skin cosmetic application potentials with the effects of removing wrinkles, whitening and protecting sun. The traditional minority medicinal plant in China, namely the chaenomeles speciosa, is effectively utilized, and the chaenomeles speciosa extracts with extremely high skin cosmetic activity are obtained. The extracts can be used for manufacturing good antioxidants, has excellent effects of removing wrinkles, whitening and protecting sun and are good natural plant cosmetic raw materials.

Description

Technical field [0001] The present invention belongs to the technical field of cosmetics and medicine, and specifically relates to the extraction of natural active substance-wrinkled papaya extract from a traditional medicinal plant wrinkled papaya commonly used in ethnic minority areas in China, and cosmetics or medicines using it as an active ingredient. And the application of wrinkled papaya extract in the preparation of moisturizing, skin rejuvenation, anti-wrinkle, sunscreen, whitening and other skin cosmetics, as well as the application in the preparation of drugs for treating or preventing skin diseases technical background [0002] Wrinkled papaya (Chaenomelesspeciosa (Sweet) Nakai), also known as papaya and iron-footed pear, is a deciduous shrub of the Rosaceae Papaya genus. The branches are erect, thorny, cylindrical, slightly buckled, glabrous, purple-brown or dark-brown , With sparse light brown lenticels. Wrinkled papaya is a temperate tree species with strong adapt...

Claims

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Application Information

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IPC IPC(8): A61K36/732A61K8/97A61P17/00A61Q19/08A61Q19/00A61Q19/02A61P39/06A61P23/00A61P31/10A61Q17/04
Inventor 杨立新许刚刘爱忠裴盛基
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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