Method for extracting total alkaloid in stem bark and leaves of nauclea offcinalis

An extraction method and a technology of total alkaloids, which are applied in the field of separation and purification of natural medicines, can solve the problems of active substance changes, losses, material consumption, energy consumption, and high working hours, so as to reduce extraction temperature and concentration temperature, reduce damage or deterioration, and improve The effect of leaching efficiency

Inactive Publication Date: 2012-07-04
海南制药厂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The existing extraction and purification technology mainly has the following deficiencies: (1) Using water as the extraction medium is economical, but the efficiency of extracting active ingredients is low, which makes it difficult to effectively extract the active ingredients in the medicinal material of Panax chinensis. The ever-decreasing medicinal materials of bile wood have not been effectively utilized; in addition, long-term boiling and concentration at higher temperatures will cause structural changes or damage to some active ingredients with poor stability, resulting in undue losses
(2) The preparation of the extract solution of the gallwood, because the stem bark of the gallwood is boiled with water contains a certain amount of colloid, there are many steps in the process, the cycle is long, the material consumption, energy consumption and working hours are high, and the yield is not ideal
Due to many steps, repeated storage under high temperature and alkaline conditions will inevitably cause changes and losses of some active substances with poor stability
(3) Tests have shown that the extracts of birchwood prepared from aqueous solutions with relatively high concentrations of alcohol have higher antibacterial activity. There are certain markets in several provinces and regions, and they have a good therapeutic effect on diseases such as colds and inflammations caused by upper respiratory tract infections. Due to the shortage of medicinal materials of birchwood, the market situation is in short supply. With the advancement of technology and the expansion of market demand, the original Some deficiencies in the preparation process need to be improved urgently

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Extraction with an acidic aqueous solution of alcohol

[0041] Grind 5kg of the stem bark of the gallwood into 30-60 mesh of the stem bark of the gallwood, put it into the extraction tank, add 10 times of the stem bark of the gallwood powder to a content of 75% (mass percentage concentration) of 3% (mass percentage concentration) of ethanol Hydrochloric acid aqueous solution, soak and extract at room temperature for 2 hours, release the leachate, soak repeatedly in the same method until the detection of vinblastam is very small, filter the extract, combine and concentrate in vacuum until almost no alcohol smell, and use 10% sodium hydroxide Adjust the pH of the solution to 6.5-7.0, continue to concentrate to a density of 1.16-1.17, refrigerate overnight and then filter to obtain 6.5 liters of concentrated solution of total alkaloids of japonica. The concentrated solution was vacuum concentrated and dried to obtain 420 grams of choline total alkaloid powder.

[0042...

Embodiment 2

[0045] (1) Extraction with an acidic aqueous solution of alcohol

[0046] Grinding 3kg of the stem bark of the gallwood into 30-60 mesh stem bark powder, putting it into the extraction tank, adding 10 times of the stem bark powder mass content of 60% (mass percentage concentration) of 3% (mass percentage concentration) of ethanol Aqueous solution of glacial acetic acid, soak and extract at room temperature for 2 hours, release the leachate, soak repeatedly in the same way until the detection of vinca lactam is very small, filter the extract, combine and concentrate in vacuum until almost no alcohol smell, adjust the solution with concentrated ammonia water pH to 6.5-7.0, then continue to concentrate to a density of 1.16-1.17, refrigerate overnight and then filter to obtain 3.6 liters of the total alkaloid concentrate of the eucalyptus. The concentrated solution was vacuum concentrated and dried to obtain 252 grams of choline total alkaloid powder.

[0047] (2) Determination o...

Embodiment 3

[0050] (1) Extraction with an acidic aqueous solution of alcohol

[0051] Grinding 3kg of the stem bark of the gallwood into 30-60 mesh stem bark powder, putting it into the extraction tank, adding 10 times the stem bark powder mass content of 45% (mass percentage concentration) and 3% (mass percentage concentration) of ethanol Sulfuric acid aqueous solution, soak and extract at room temperature for 2 hours, release the leachate, soak repeatedly in the same method until the detection of vinca lactam is very small, filter the extract, combine and concentrate in vacuum until almost no alcohol smell, and use 10% potassium hydroxide Adjust the pH of the solution to 6.5-7.0, continue to concentrate to a density of 1.16-1.17, refrigerate overnight and then filter to obtain 3.5 liters of the total alkaloid concentrate of the eucalyptus. The concentrated solution was vacuum concentrated and dried to obtain 226 grams of choline total alkaloid powder.

[0052] (2) Determination of the ...

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Abstract

The invention relates to a method for extracting total alkaloid in stem bark and leaves of nauclea offcinalis, which comprises the steps of (1) performing filtering extraction by utilizing acid water solution of alcohol, (2) preparing total alkaloid concentrated liquid, (3) preparing total alkaloid dry powder or dry extraction, and (4) measuring total alkaloid content. The method for extracting the total alkaloid in the stem bark and the leaves of the nauclea offcinalis has the advantages of (1) reducing extraction temperature and concentration temperature, reducing heated time of the extraction, saving energy consumption, improving leaching efficiency, leaching ratio and leaching amount of active components of the alkaloid, avoiding or reducing damage or deterioration of the alkaloid with poor stability, and being capable of effectively obtaining the total alkaloid extraction or the total alkaloid dry powder of the nauclea offcinalis; (2) reducing preparation procedures and loss of the total alkaloid; (3) enabling the density of the total alkaloid obtained by the acid water solution of the alcohol to be improved by one third and enabling the types of active alkaloid to be increased by over one time; and (4) providing a simple measurement method for measuring the total alkali in the extraction of the nauclea offcinalis, and selecting wavelength scope of measurement of the total alkaloid.

Description

[0001] technical field [0002] The invention belongs to the technical field of separation and purification of natural medicines, and in particular relates to a method for extracting total alkaloids in bark and leaves of birchwood. [0003] Background technique [0004] Gall wood, also known as medicine black sandalwood, mountain bear bile, bear bile tree, fine-leaved yellow tree, yellow sheep wood, etc. for madder [0005] Dry stems, branches, trees of Nauclea Officinalis Pierrc ex Pitard [0006] bark and roots. Gallwood is bitter and cold in nature and has the effects of clearing away heat and detoxification, reducing swelling and alleviating pain. my country's Guangxi, Guangdong and Hainan folks are commonly used in the treatment of colds, fever, pneumonia, enteritis, dysentery, eczema, rashes, abscesses and other diseases. At present, there are four kinds of traditional Chinese medicine preparations: "Danmu injection", "Danmu extract tablet", "Danmu extract capsule...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/74A61P11/00A61P11/04A61P27/02A61K127/00A61K135/00
Inventor 蒲含林李秀峰赖潜李争光
Owner 海南制药厂有限公司
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