Method for extracting total alkaloid in stem bark and leaves of nauclea offcinalis

Abstract

The invention relates to a method for extracting total alkaloid in stem bark and leaves of nauclea offcinalis, which comprises the steps of (1) performing filtering extraction by utilizing acid water solution of alcohol, (2) preparing total alkaloid concentrated liquid, (3) preparing total alkaloid dry powder or dry extraction, and (4) measuring total alkaloid content. The method for extracting the total alkaloid in the stem bark and the leaves of the nauclea offcinalis has the advantages of (1) reducing extraction temperature and concentration temperature, reducing heated time of the extraction, saving energy consumption, improving leaching efficiency, leaching ratio and leaching amount of active components of the alkaloid, avoiding or reducing damage or deterioration of the alkaloid with poor stability, and being capable of effectively obtaining the total alkaloid extraction or the total alkaloid dry powder of the nauclea offcinalis; (2) reducing preparation procedures and loss of the total alkaloid; (3) enabling the density of the total alkaloid obtained by the acid water solution of the alcohol to be improved by one third and enabling the types of active alkaloid to be increased by over one time; and (4) providing a simple measurement method for measuring the total alkali in the extraction of the nauclea offcinalis, and selecting wavelength scope of measurement of the total alkaloid.

Description

[0001] technical field

[0002] The invention belongs to the technical field of separation and purification of natural medicines, and in particular relates to a method for extracting total alkaloids in bark and leaves of birchwood.

[0003] Background technique

[0004] Gall wood, also known as medicine black sandalwood, mountain bear bile, bear bile tree, fine-leaved yellow tree, yellow sheep wood, etc. for madder

[0005] Dry stems, branches, trees of Nauclea Officinalis Pierrc ex Pitard

[0006] bark and roots. Gallwood is bitter and cold in nature and has the effects of clearing away heat and detoxification, reducing swelling and alleviating pain. my country's Guangxi, Guangdong and Hainan folks are commonly used in the treatment of colds, fever, pneumonia, enteritis, dysentery, eczema, rashes, abscesses and other diseases. At present, there are four kinds of traditional Chinese medicine preparations: "Danmu injection", "Danmu extract tablet", "Danmu extract capsule...

Examples

Embodiment 1

[0040] (1) Extraction with an acidic aqueous solution of alcohol

[0041] Grind 5kg of the stem bark of the gallwood into 30-60 mesh of the stem bark of the gallwood, put it into the extraction tank, add 10 times of the stem bark of the gallwood powder to a content of 75% (mass percentage concentration) of 3% (mass percentage concentration) of ethanol Hydrochloric acid aqueous solution, soak and extract at room temperature for 2 hours, release the leachate, soak repeatedly in the same method until the detection of vinblastam is very small, filter the extract, combine and concentrate in vacuum until almost no alcohol smell, and use 10% sodium hydroxide Adjust the pH of the solution to 6.5-7.0, continue to concentrate to a density of 1.16-1.17, refrigerate overnight and then filter to obtain 6.5 liters of concentrated solution of total alkaloids of japonica. The concentrated solution was vacuum concentrated and dried to obtain 420 grams of choline total alkaloid powder.

[0042...

Embodiment 2

[0045] (1) Extraction with an acidic aqueous solution of alcohol

[0046] Grinding 3kg of the stem bark of the gallwood into 30-60 mesh stem bark powder, putting it into the extraction tank, adding 10 times of the stem bark powder mass content of 60% (mass percentage concentration) of 3% (mass percentage concentration) of ethanol Aqueous solution of glacial acetic acid, soak and extract at room temperature for 2 hours, release the leachate, soak repeatedly in the same way until the detection of vinca lactam is very small, filter the extract, combine and concentrate in vacuum until almost no alcohol smell, adjust the solution with concentrated ammonia water pH to 6.5-7.0, then continue to concentrate to a density of 1.16-1.17, refrigerate overnight and then filter to obtain 3.6 liters of the total alkaloid concentrate of the eucalyptus. The concentrated solution was vacuum concentrated and dried to obtain 252 grams of choline total alkaloid powder.

[0047] (2) Determination o...

Embodiment 3

[0050] (1) Extraction with an acidic aqueous solution of alcohol

[0051] Grinding 3kg of the stem bark of the gallwood into 30-60 mesh stem bark powder, putting it into the extraction tank, adding 10 times the stem bark powder mass content of 45% (mass percentage concentration) and 3% (mass percentage concentration) of ethanol Sulfuric acid aqueous solution, soak and extract at room temperature for 2 hours, release the leachate, soak repeatedly in the same method until the detection of vinca lactam is very small, filter the extract, combine and concentrate in vacuum until almost no alcohol smell, and use 10% potassium hydroxide Adjust the pH of the solution to 6.5-7.0, continue to concentrate to a density of 1.16-1.17, refrigerate overnight and then filter to obtain 3.5 liters of the total alkaloid concentrate of the eucalyptus. The concentrated solution was vacuum concentrated and dried to obtain 226 grams of choline total alkaloid powder.

[0052] (2) Determination of the ...

[0001] technical field

[0002] The invention belongs to the technical field of separation and purification of natural medicines, and in particular relates to a method for extracting total alkaloids in bark and leaves of birchwood.

[0003] Background technique

[0004] Gall wood, also known as medicine black sandalwood, mountain bear bile, bear bile tree, fine-leaved yellow tree, yellow sheep wood, etc. for madder

[0005] Dry stems, branches, trees of Nauclea Officinalis Pierrc ex Pitard

[0006] bark and roots. Gallwood is bitter and cold in nature and has the effects of clearing away heat and detoxification, reducing swelling and alleviating pain. my country's Guangxi, Guangdong and Hainan folks are commonly used in the treatment of colds, fever, pneumonia, enteritis, dysentery, eczema, rashes, abscesses and other diseases. At present, there are four kinds of traditional Chinese medicine preparations: "Danmu injection", "Danmu extract tablet", "Danmu extract capsule...

Examples

Embodiment 1

[0040] (1) Extraction with an acidic aqueous solution of alcohol

[0041] Grind 5kg of the stem bark of the gallwood into 30-60 mesh of the stem bark of the gallwood, put it into the extraction tank, add 10 times of the stem bark of the gallwood powder to a content of 75% (mass percentage concentration) of 3% (mass percentage concentration) of ethanol Hydrochloric acid aqueous solution, soak and extract at room temperature for 2 hours, release the leachate, soak repeatedly in the same method until the detection of vinblastam is very small, filter the extract, combine and concentrate in vacuum until almost no alcohol smell, and use 10% sodium hydroxide Adjust the pH of the solution to 6.5-7.0, continue to concentrate to a density of 1.16-1.17, refrigerate overnight and then filter to obtain 6.5 liters of concentrated solution of total alkaloids of japonica. The concentrated solution was vacuum concentrated and dried to obtain 420 grams of choline total alkaloid powder.

[0042...

Embodiment 2

[0045] (1) Extraction with an acidic aqueous solution of alcohol

[0046] Grinding 3kg of the stem bark of the gallwood into 30-60 mesh stem bark powder, putting it into the extraction tank, adding 10 times of the stem bark powder mass content of 60% (mass percentage concentration) of 3% (mass percentage concentration) of ethanol Aqueous solution of glacial acetic acid, soak and extract at room temperature for 2 hours, release the leachate, soak repeatedly in the same way until the detection of vinca lactam is very small, filter the extract, combine and concentrate in vacuum until almost no alcohol smell, adjust the solution with concentrated ammonia water pH to 6.5-7.0, then continue to concentrate to a density of 1.16-1.17, refrigerate overnight and then filter to obtain 3.6 liters of the total alkaloid concentrate of the eucalyptus. The concentrated solution was vacuum concentrated and dried to obtain 252 grams of choline total alkaloid powder.

[0047] (2) Determination o...

Embodiment 3

[0050] (1) Extraction with an acidic aqueous solution of alcohol

[0051] Grinding 3kg of the stem bark of the gallwood into 30-60 mesh stem bark powder, putting it into the extraction tank, adding 10 times the stem bark powder mass content of 45% (mass percentage concentration) and 3% (mass percentage concentration) of ethanol Sulfuric acid aqueous solution, soak and extract at room temperature for 2 hours, release the leachate, soak repeatedly in the same method until the detection of vinca lactam is very small, filter the extract, combine and concentrate in vacuum until almost no alcohol smell, and use 10% potassium hydroxide Adjust the pH of the solution to 6.5-7.0, continue to concentrate to a density of 1.16-1.17, refrigerate overnight and then filter to obtain 3.5 liters of the total alkaloid concentrate of the eucalyptus. The concentrated solution was vacuum concentrated and dried to obtain 226 grams of choline total alkaloid powder.

[0052] (2) Determination of the ...