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Pseudomonas sp. 841P-3 for preventing cotton verticillium wilt and use thereof

A technology for cotton verticillium wilt and pseudomonas, applied in the field of pseudomonas, can solve the problems of difficulty in prevention and control, difficulty in achieving ideal control effects with disease control methods and agents, difficulty in finding resistant materials, etc., achieving remarkable results, The effect of good biopesticide development prospects

Inactive Publication Date: 2013-04-10
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Verticillium wilt of cotton is a soil-borne vascular disease. It is difficult to control and find good resistant materials. Therefore, it has not been possible to breed new cotton varieties with high resistance to Verticillium wilt for many years. Difficult to achieve ideal control effect

Method used

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  • Pseudomonas sp. 841P-3 for preventing cotton verticillium wilt and use thereof
  • Pseudomonas sp. 841P-3 for preventing cotton verticillium wilt and use thereof
  • Pseudomonas sp. 841P-3 for preventing cotton verticillium wilt and use thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The acquisition of embodiment 1 Pseudomonas 841P-3

[0027] 1.1 Isolation of bacteria

[0028] Take healthy flower roots of cotton M-8124-1159 at the budding stage, use a sterilized knife to take the main root at 5-10 cm, immerse in 70% alcohol for 1 min, and sterilize the surface of the sample with 1.05% sodium hypochlorite for 15-18 min. Take 1.00g of the material that has been thoroughly sterilized on the surface of the test, put it in a sterile mortar, add sterilized PBS buffer solution to grind, let it stand for 15 minutes, add buffer solution to the volume to 10mL, take 1ml of the separation solution and take 0.1ml for coating In prepared S2 (agar 18g, sucrose 10g, glycerol 10ml, casein 5g, sodium bicarbonate 1g, MgSO 4 ·7H 2 O 1g, K 2 HPO 4 2.3g, sodium lauroyl sarcosinate 1.2g, deionized water to 1L) culture medium, used for the isolation of Pseudomonas, three groups in parallel, cultured at 28°C for 2 to 3 days, and counted the number of colonies. Pick a ...

Embodiment 2

[0036] Embodiment 2 Antagonism mechanism research:

[0037] Pick the hyphae around the antagonistic bacteria Pseudomonas 841P-3 (CGMCC No.5583) in the confrontation culture and collect the spores around the antagonistic bacteria for microscopic examination to observe the growth of the mycelium and the germination of the spores. The result shows that the hyphae of Verticillium dahliae V107 around Pseudomonas 841P-3 (CGMCCNo.5583) appear deformed, enlarged, abnormal growth, spore rupture, and spore germination is inhibited ( Figure 4 , 5).

Embodiment 3

[0038] Example 3 Induced Resistance Verification

[0039] 3.1 Improving cotton young root resistance

[0040] Preparation of cell suspension: Inoculate Pseudomonas 841P-3 (CGMCC No.5583) strain in liquid LB, culture at 37°C for 24 hours, centrifuge to remove supernatant, resuspend in sterile water, and dilute to OD 600 =0.1 bacterial suspension. After soaking and sterilizing cotton seeds for 30 minutes, and culturing them at 28°C for 48 hours, they were transferred to PDA plates that had been grown for 2 days and connected with Verticillium dahliae V107, and the degree of injury was observed after 7 days of co-cultivation. The results showed that the degree of damage to the young roots of Pseudomonas 841P-3 (CGMCC No.5583) after soaking was significantly smaller than that of water-treated roots, such as Image 6 .

[0041] 3.2 Improve leaf resistance

[0042] The concentration of bacteria suspension is OD 600 After =0.2 Pseudomonas 841P-3 (CGMCC No.5583) root irrigation p...

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Abstract

The invention belongs to the technical field of biopesticides and relates to pseudomonas sp. 841P-3 for preventing cotton verticillium wilt and use thereof. The pseudomonas sp. 841P-3 was preserved in China General Microbiological Culutre Collection Center in December 13th, 2011. The collection number of the pseudomonas sp. 841P-3 is CGMCC No.5583. The invention also discloses the use of the pseudomonas sp. 841P-3 with the collection number being CGMCC No.5583 in prevention and control of cotton verticillium wilt. Through microbial fermentation, lots of pseudomonas sp. 841P-3 strains can be obtained. The strains can produce resistance to induction system, are safe to human beings, animals and crops, are environment-friendly, have obvious cotton verticillium wilt prevention and control effect and good biopesticide development prospect.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and relates to a pseudomonas strain used for preventing and treating verticillium wilt of cotton and its application. Background technique [0002] Cotton Verticillium wilt is a fungal vascular system disease caused by Verticillium dahliae infection, and it is one of the most serious diseases of cotton. The mycelium of Verticillium dahliae can produce a large number of micro-sclerotias. These micro-sclerotias survive in the soil for a long time, have a wide range of hosts, and have many transmission routes, which seriously threaten cotton production and cause serious losses to cotton production in my country. So far there is no effective control method. Verticillium wilt of cotton is a soil-borne vascular disease, and it is difficult to control and find good resistant materials. Therefore, new cotton varieties with high resistance to Verticillium wilt have not been bred for many ye...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/38
Inventor 唐灿明赵明文韩琴李顺鹏
Owner NANJING AGRICULTURAL UNIVERSITY