Method for preparing rapeseed active polypeptides

An active polypeptide and rapeseed technology, applied in the field of food processing, can solve the problems of protein denaturation, development and utilization restrictions, etc., and achieve the effects of low antigenicity, good acid solubility, and improvement of learning and memory ability.

Active Publication Date: 2012-07-04
ANHUI YANZHIFANG FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Generally speaking, the amino acid composition of rapeseed protein is reasonable, close to the amino acid composition model recommended by the Food and Agriculture Organization of the United Nations (WHO) and the World Health Organization (FAO), but the high-temperature oil extraction process will cause protein denaturation. There are more or less anti-nutritional factors in rapeseed crude protein, which limits its development and utilization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Preparation of rapeseed active polypeptide

[0022] (1) Take the dehulled defatted dry rapeseed meal (crude protein content 38.5%), crush it through a 60-mesh sieve and place it in an extraction tank, add 10 times the amount of distilled water, stir and extract at 40±2℃ for 1h, 6000rpm, 4 Centrifuge at ℃ for 20 minutes, filter the supernatant to obtain the precipitate, perform the same extraction operation 3 times according to the above steps, combine the obtained supernatants, precipitate the supernatant with 1mol / L hydrochloric acid, adjust the pH to 4.0 and then 6000rpm, 4℃ Centrifuge for 10 minutes to obtain the precipitate, place the precipitate in distilled water for 24 hours, and replace the distilled water every 8 hours, and then vacuum freeze-dry to obtain rapeseed albumin;

[0023] (2) Prepare rapeseed albumin with distilled water to prepare a protein solution with a mass fraction of 6%, add 4000U of Flavourzyme protease per gram of rapeseed albumin, main...

Embodiment 2

[0026] Example 2 Experimental study of rapeseed active polypeptide promoting memory in mice

[0027] Six-week-old healthy mice (weight 22g-25g, male and female), the test mice were randomly divided into 3 groups, 15 in each group, namely the young control group, the aging model group, and the active peptide treatment group. Among them, the mice in the aging model group and the active peptide treatment group were subcutaneously injected with 0.5ml / 20g (200mg / kg) of 0.8% D-galactose solution diluted with physiological saline to the neck and back of the mice, once a day for 6 consecutive weeks, resulting in the aging model The young control group was injected with the same amount of physiological saline subcutaneously, and the active peptide treatment group was gavage once a day at nine o’clock in the morning while building the model. The rapeseed active polypeptide prepared in Example 1 of the present invention was dosed at 0.8 g / kg body weight. Dilute with normal saline for 6 wee...

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PUM

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Abstract

The invention provides a method for preparing rapeseed active polypeptides. The method comprises the following steps of: grinding dehusked, defatted and dried rapeseeds, leaching with water, centrifuging, precipitating the resulting supernatant with acids, dialyzing in water, hydrolyzing the resulting rapeseed albumin with two enzymes, decolorizing, and removing foreign matters, to obtain rapeseed active polypeptides. The method has the advantages of simple production process, mild reaction condition and no strict requirement for production equipment, and is suitable for industrial production.

Description

Technical field [0001] The invention is a food processing technology field, involving a preparation method and application of rapeseed activated polypeptides. [0002] Background technique [0003] Rapeseed is one of the important oil crops, and my country's rape production ranks first in the world.Under normal circumstances, rapeseed can get about 3506 rapeseed oil and about 65%rapeseed meal.Rapeseed meal is rich in crude protein, crude fat, lecithin, a variety of vitamins, amino acids, etc., which can digest a protein content of 27.8%.In addition, rapeseed meal also contains B vitamins and CA, P, Fe, CU, Zn, Mn, etc. B, P, FE, Zn, Mn, etc.element.The crude protein content of rapeseed meal is generally 35%-45%. It is a high-quality full-cost plant protein. It has important economic significance and social significance for development and utilization.However, the current use of rapeseed meal in my country is quite limited. It is only used as fertilizer or a small amount of baids ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/14A61K38/01A61P25/00A23L1/29A23L1/305A23L33/00
Inventor 张丽琍祁斌吴雷张超
Owner ANHUI YANZHIFANG FOOD
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