Method for rapidly detecting non-dairy creamer in adulterated fresh milk

A lipid-based, fast technology, applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., can solve problems such as poor specificity, and achieve the effects of low cost, rapid detection, and suitable sensitivity

Inactive Publication Date: 2013-08-28
BEIJING FUTONGHUA INVESTMENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, for the detection of non-dairy creamer mixed with raw milk, it is judged that a small amount of palm oil complexes will float in the milk sample when non-dairy creamer and oil powder in fresh milk are cold, and cooperate with the detection of whether there is Sugars are present, but such detection methods are less specific

Method used

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  • Method for rapidly detecting non-dairy creamer in adulterated fresh milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The detection reagent of non-dairy creamer in embodiment 1 milk

[0023] Preparation of liquid A:

[0024] Add 400ml of high-purity water into a 1000ml beaker (the resistivity is required to be above 18M), put it on a magnetic stirrer, and add 2g of hydroxymethylcellulose sodium salt (CMC-requires white appearance, low molecular weight, and low viscosity) while stirring, 3.5 g of anhydrous citric acid (analytically pure) and 4.25g of a crystalline water sodium citrate (analytical pure), heated to 60-65°C, stirred for about 20 minutes to dissolve completely, stopped heating, and when the solution dropped to room temperature, added while stirring 2ml 10% RHODAFAC RE960 stabilizer (nonylphenol phosphate), 4g glucose oxidase (126U / mg) (purchased from Sigma, USA), 1g peroxidase (114U / mg) (purchased from Sigma, USA), 10ml 2% thiazole yellow (purchased from Sigma, USA) was stirred for about 30 minutes to dissolve completely, and the volume was adjusted to 500 ml with high-pur...

Embodiment 2

[0027] The detection reagent of non-dairy creamer in embodiment 2 milk

[0028] Preparation of liquid A:

[0029] Add 400ml of high-purity water into a 1000ml beaker (the resistivity is required to be above 18M), put it on a magnetic stirrer, and add 4g of hydroxymethylcellulose sodium salt (CMC-requires white appearance, low molecular weight, and low viscosity) while stirring, 3.5 g of anhydrous citric acid (analytically pure) and 4.25g of a crystalline water sodium citrate (analytical pure), heated to 60-65°C, stirred for about 20 minutes to dissolve completely, stopped heating, and when the solution dropped to room temperature, added while stirring 2ml10% triphenyl phosphite, 2g glucose oxidase (126U / mg) (purchased from Sigma, U.S.), 0.7g peroxidase (114U / mg) (purchased from Sigma, U.S.), 40ml2% reactive yellow (purchased from Sigma, U.S. (from the U.S. Sigma Company), stirred for about 30 minutes to dissolve completely, and dilute to 500 ml with high-purity water, and set...

Embodiment 3

[0032] The detection reagent of non-dairy creamer in embodiment 3 milk

[0033] Preparation of liquid A:

[0034] Add 400ml of high-purity water into a 1000ml beaker (resistivity must be above 18M), put it on a magnetic stirrer, and add 6g of polyvinyl alcohol, 3.5g of anhydrous citric acid (analytical pure) and 4.25g of a crystalline water of citric acid while stirring Sodium (analytical pure), heated to 60-65 ° C, stirred for about 20 minutes to dissolve completely, stop heating, after the solution has cooled to room temperature, add 2ml 10% RHDAFAC RE960 stabilizer, 3g glucose oxidase (126U / mg) while stirring (purchased from U.S. Sigma Company), 1.5g peroxidase (114U / mg) (purchased from U.S. Sigma Company), 20ml 2% acid yellow (purchased from U.S. Sigma Company), stirred for about 30min to dissolve completely, and settled to volume with high-purity water to 500 ml, set aside.

[0035] Preparation of liquid B:

[0036] Add 300ml anhydrous toluene (reagent grade) in the 10...

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Abstract

The invention belongs to the field of food detection, and in particular relates to a detection method of milk, particularly to detection of non-dairy creamer in adulterated fresh milk. A detection reagent for rapidly detecting the non-dairy creamer in the milk disclosed in the invention is a solution A and a solution B, wherein the solution A comprises 2-10g / L thickener, 1*10<5>-1*10<7>U / L glucose oxidase, 1*10<5>-1*10<6>U / L horseradish peroxidase, 0.3-0.5g / L yellow dye and water in balance, and has pH of 5.0-7.5; and the solution B comprises 6-10g / L tetramethylbenzidine, and organic solvent in balance. The invention also discloses a test strip containing the reagent and a rapid detection of non-dairy creamer in adulterated fresh milk by using the test strip. The method in the invention has a simple and fast operation, appropriate sensitivity and low cost, and can rapidly detect non-dairy creamer in fresh milk.

Description

technical field [0001] The invention belongs to the field of food detection, and in particular relates to a detection method of milk, in particular to detection of adulteration of non-dairy creamer in fresh milk. Background technique [0002] Non-dairy creamer, also known as creamer, is a condiment produced from hydrogenated vegetable oil, corn syrup or glucose syrup, emulsifier, anti-caking agent, etc. It has a strong milk aroma and a fat content of about 20%-75%. , so the heat production is generally higher. Since dairy companies purchase raw milk based on the milk fat percentage, dairy farmers add non-dairy creamer to the raw milk in order to increase the milk fat percentage of fresh milk. Cheap and low-quality non-dairy creamer contains harmful substances such as arsenic (arsenic), lead, and copper. Long-term intake can increase the chances of suffering from coronary heart disease, tumors, asthma and other diseases, and children will even become mentally retarded. [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
Inventor 张宏强
Owner BEIJING FUTONGHUA INVESTMENT
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