Disposable cell passage, blowing, beating and subpackaging suction tube

A one-time, pipette technology, applied in the field of one-time cell passaging, dispensing pipettes, blowing and beating, it can solve the problems of cell impact force, easy generation of foam, affecting cell passaging, etc., so as to reduce the pressing force, reduce damage, and facilitate entry and exit. bottle mouth effect

Active Publication Date: 2012-07-18
QIANFOSHAN HOSPITAL OF SHANDONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This caused a large piece of cells at the bottom of the culture flask to fall off when blown, and there was no way to become a single-cell suspension.
In addition, at the two upper shoulders of the culture bottle, the straight tips cannot be blown to, resulting in the cells at the two shoulders being unable to be passaged, wh

Method used

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  • Disposable cell passage, blowing, beating and subpackaging suction tube
  • Disposable cell passage, blowing, beating and subpackaging suction tube
  • Disposable cell passage, blowing, beating and subpackaging suction tube

Examples

Experimental program
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Embodiment 1

[0019] Such as figure 1 As shown, a disposable straw for cell passage, blowing, and dispensing, which includes a straw body 1, the rear end of the straw body 1 is a balloon 2, and the front end is a suction nozzle 3, and the straw body 1 is provided with a curved connecting portion 4 . The bending angle α between the front end of the straw and the rear end of the straw (the included angle between the extension lines of the center lines of the two) is 45°. The straw main body 1 is integrally formed by plastic material.

Embodiment 2

[0021] Such as figure 2 shown, as figure 1 As shown, a disposable straw for cell passage, blowing, and dispensing includes a straw main body 1 integrally formed of plastic materials. The back end of the straw main body 1 is a balloon 2, and the front end is a suction nozzle 3. The suction nozzle 3 is also a section of straight pipe. It is composed of ring-shaped undulating folds 5 in a folded state. The bending angle α between the front end of the straw and the rear end of the straw is between 0° and 180°.

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PUM

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Abstract

The invention discloses a disposable cell passage, blowing, beating and subpackaging suction tube which comprises a suction tube body, wherein the back end of the suction tube is provided with a saccule while the front end per se is provided with a suction nozzle; and the suction tube body is provided with a bended connecting part. The suction tube is folded to have a certain angle according to a need to enable the suction nozzle to point towards the direction of a flask bottom, so that cells at the flask bottom can be blown and beaten conveniently and unicellular suspension is easier to form. In addition, as the suction nozzle points towards the direction of the flask bottom, pressing force degree of a finger can be lightened, foams which are generated as a liquid impacts the flask bottom vigorously are reduced, and thereby damage from the foams to the cells is also reduced; cells at two upper shoulder parts of a culture flask can be blown down, so that the disadvantage that the two upper shoulder parts of the culture flask cannot be brown by a straight suction tube is avoided; as the angle of the suction tube can be adjusted randomly and the suction tube before absorbing the cell suspension and subpackaging can be flipped and straightened, so that a dropper can be put in or taken out of a flask opening conveniently for transferring and subpackaging of the cell suspension; and the suction tube can reach all the parts of the culture flask to adsorb and scrape objects on the inner wall of the culture flask.

Description

[0001] technical field [0002] The invention relates to a disposable cell passaging, blowing and dispensing pipette. Background technique [0003] In the field of clinical treatment and biological research, cell culture is indispensable. When cells proliferate to a certain extent, contact inhibition occurs. In order to allow cells to continue to grow and expand the number of cells, it is necessary to divide from one culture flask to different culture flasks. At present, straws are often used for cell subculture, blowing, and dispensing, and the suckers include a main body of the straw, a suction nozzle and a long balloon. When in use, squeeze the elongated balloon part with your hands, absorb the liquid in the culture bottle, blow and beat the cells at the bottom of the culture bottle for passage, and transfer the blown cell suspension into aliquots. [0004] The tip of the pipette currently used in cell subculture and subpackaging is straight, and when the cells in the c...

Claims

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Application Information

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IPC IPC(8): C12M1/24
CPCC12M23/28C12M33/04
Inventor 杜娟孙青曹莉莉岳龙涛孙宗国
Owner QIANFOSHAN HOSPITAL OF SHANDONG
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