Human immunodeficiency virus affinity adsorption column, and preparation method and uses thereof

An HIV and adsorption column technology is applied in the field of rapid and efficient removal of HIV from blood, which can solve the problems of ineffective immunotherapy, difficult opportunistic treatment, frequent antiviral treatment effects, etc. The effect of short treatment time and large toxic and side effects

Inactive Publication Date: 2012-08-15
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0021] Generally speaking, antiviral therapy has frequent curative effects, high toxic and side effects, easy to produce drug-resistant strains, and high cost; immunotherapy has indirect effect, poor specificity, ineffective treatment, and opportunistic treatment is difficult and periodic. Long, easy to control and difficult
At present, there is still a lack of a low-side effect, low-cost and effective means of treating AIDS

Method used

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  • Human immunodeficiency virus affinity adsorption column, and preparation method and uses thereof
  • Human immunodeficiency virus affinity adsorption column, and preparation method and uses thereof
  • Human immunodeficiency virus affinity adsorption column, and preparation method and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Cloning, expression and purification of embodiment 1 affinity protein

[0055] Affinity proteins include CD4 molecules, CCR-5, CXCR-4, and gp120 antibodies, all of which can be expressed by eukaryotic yeast expression systems. The following is an example of the main receptor CD4 molecule.

[0056] Human CD4 molecule is a kind of single-chain transmembrane glycoprotein with a relative molecular weight of about 55kDa, which belongs to the immunoglobulin superfamily and is mainly expressed in some T cells, thymocytes, some B cells and Epstein-Barr virus-transformed B cells, single Nuclei - Macrophages and the surface of brain cells in specific areas. It plays an important role in the development of T cells, the activation of mature T cells, and signal transduction. It is an important effector molecule in the occurrence of adaptive immune responses and plays a huge role in maintaining the normal physiological functions of the body.

[0057] The human CD4 molecular cDNA ...

Embodiment 2

[0148] The preparation of embodiment 2 immunoadsorption microspheres

[0149] principle:

[0150] The affinity microspheres can be glass microspheres with a size of 1 mm, chitosan cross-linked microspheres with a diameter greater than 500 μm, or agarose microspheres.

[0151] In this experiment, the choice of particle size can also choose 1mm glass beads as the carrier material. Due to their strong surface area and pore structure, glass beads have the potential to immobilize various ligands. Moreover, glass beads also have relatively good blood compatibility, so they can also be considered as a carrier for adsorption. Before protein immobilization, glass beads need to be pretreated such as washing. During the preparation process, glass beads are often mixed with impurities such as dust and grease, so they need to be cleaned. In this experiment, the glass beads were placed in a solution of 95% acetone and ultrasonicated for 5 minutes to remove impurities from the glass be...

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Abstract

The invention discloses a human immunodeficiency virus (HIV) affinity adsorption column, comprising a column body and at least one activated affinity microsphere located in the column body, wherein the activated affinity microsphere is connected with a human immunodeficiency virus affinity protein, and the affinity protein can be bound with human immunodeficiency virus. The affinity protein comprises a main receptor CD4 molecule, a gp120 antibody, an auxiliary receptor CXC chemokine receptor 4 (CXCR-4) and a CC chemokine receptor 5 (CCR-5). The affinity microsphere may be a glass microsphere with the size of 1mm, a chitosan crosslinking microsphere with the diameter more than 500 microns, or a gluosan microsphere. The human immunodeficiency virus affinity adsorption column disclosed by the invention is applicable for eliminating the human immunodeficiency virus in the blood of HIV patients, and relieving and treating the immunodeficiency syndrome of HIV patients; and compared with the traditional treatment methods, the immunoadsorption column has high safety, good specificity, small toxic or side effects and good operation simplicity.

Description

technical field [0001] The invention relates to a rapid and efficient removal treatment technology for HIV causing human immunodeficiency syndrome from blood, in particular to a protein affinity adsorption column, which can specifically remove HIV particles in blood and characterize patients after treatment and quantitative detection. Background technique [0002] HIV [0003] HIV (AIDS virus) is officially named Human Immunodeficiency Virus (Human Immunodeficiency Virus) and belongs to the Lentiviridae subfamily of Retroviridae. About 110nm, the viral core contains two identical nucleic acid strands and viral reverse transcriptase (DNA polymerase, nuclease, integrase). The nucleic acid type is single-stranded positive-strand RNA, which can be used as a template for reverse transcription into cDNA and as mRNA to directly translate proteins. The genome size is about 9.3kb. The genome is flanked by long terminal repeats (LTRs), 3 structural genes (gag, pol, env) and at leas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/30B01D15/08A61M1/38
Inventor 王业富刘红莲王曼
Owner WUHAN UNIV
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