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Test tube cottage method for huperzia serrata sporophyte

A technology of Huperzia serrata and sporophyte, which is applied in the field of test tube cutting of Huperzia serrata sporophyte, can solve the problems of cutting or transplanting experiment failure, and achieve the effect of accelerating propagation

Inactive Publication Date: 2013-07-10
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, cutting experiments of Huperz serrata can only be carried out in artificial climate chambers or areas with high humidity, while related cutting or transplanting experiments in western China have all ended in failure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of culture substrate

[0029] Fully soak the dried sphagnum moss in 1 / 2MS culture solution containing 0.5 mg / L 3-indolebutyric acid (IBA), then put it into a wide-mouth culture bottle up to 1 / 3 volume, and seal it with bottle sealing film. Seal the mouth of the bottle, sterilize at 121°C for 25 minutes, and let it cool for later use;

[0030] (2) Surface sterilization of materials

[0031] Fresh materials of Huperzia serrata (Thunb.) Trev. were collected from the wild, rinsed with tap water, cut off 6 cm terminal buds, and immersed in an ethanol solution with a mass fraction of 75% for 30 seconds in an ultra-clean workbench; At a concentration of 0.1% mercury chloride (HgCl 2 ) solution for 8 minutes, then rinsed with sterile water for 3 times, as cutting material;

[0032] (3) Cutting culture of sporophytes

[0033] The terminal bud cuttings were placed in a sterilized culture bottle, and the culture temperature was 22±2°C, and the light time was 16...

Embodiment 2

[0037] (1) Preparation of culture substrate

[0038] Fully soak the dried water moss in 1 / 2MS culture solution containing 0.5mg / L 3-indolebutyric acid, then put it into a wide-mouth culture bottle up to 1 / 3 of its volume, and seal the bottle mouth with a sealing film Live, sterilize at 121°C for 25 minutes, and let it cool for later use;

[0039] (2) Surface sterilization of materials

[0040] Fresh materials of Huperzia serrata (Thunb.) Trev. were collected from the wild, rinsed with tap water, cut off 8 cm terminal buds, and immersed in an ethanol solution with a mass fraction of 75% for 60 seconds in an ultra-clean workbench; Mercury chloride (HgCl) at a concentration of 0.1% 2 ) solution for 5 minutes, then rinsed with sterile water for 3 times, as cutting material;

[0041] (3) Cutting culture of sporophytes

[0042] Cuttings of the terminal buds were placed in a culture bottle, cultured for 12 weeks at a culture temperature of 22 ± 2°C and a light time of 12 h / d. The...

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PUM

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Abstract

The invention discloses a test tube cottage method for huperzia serrata sporophyte. The method uses terminal buds of fresh huperzia serrata sporophyte as materials, and reproduction and preservation of the materials are performed in a test tube cottage mode. The method is simple, easy to operate and high in efficiency, has important significance to preservation of the material resources of huperzia serrata and can provide fresh and alive experimental materials for laboratory studies on relevant aspects in various regions.

Description

technical field [0001] The invention belongs to the technical field of asexual reproduction, and in particular relates to a method for cutting huperzia serrata sporophytes in test tubes. Background technique [0002] Huperzia serrata (Thunb.) Trev., also known as Melaleuca pagoda, Sanzhi and snake foot grass, etc., is a perennial herbaceous fern of the Huperoaceae Huperoaceae Huperzia Bernh. The whole herb is mainly used in Chinese folk. It is used to treat diseases such as bruises, congestion, swelling and pain, and schizophrenia. Since my country's scientific and technological workers first reported that huperzine A isolated from this plant has a striated muscle relaxation effect in 1972, a large number of studies have been carried out on it, and it has been found that huperzine A is a highly efficient, low-toxic, reversible and highly selective It is a potent acetylcholinesterase inhibitor, which has a significant effect on the treatment of Alzheimer's disease (AD), myast...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 包日双郭斌尉亚辉
Owner NORTHWEST UNIV
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