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Method for preparing astragalus polysaccharide selenide by using organic method

A technique for the selenization of astragalus polysaccharides, which is applied in the field of organic preparation of selenized astragalus polysaccharides, can solve the problems of low stability of selenized astragalus polysaccharides, reduce the selenium activation function, reduce the amount of selenizing agent, and shorten the selenization reaction time Effect

Inactive Publication Date: 2014-02-26
浙江博信药业股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] However, this method does not involve how to extract astragalus polysaccharides with a higher yield, and the prepared selenized astragalus polysaccharides are not stable

Method used

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  • Method for preparing astragalus polysaccharide selenide by using organic method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation of astragalus polysaccharides: Weigh 1kg of astragalus polysaccharide powder, use water as a solvent according to the material-to-liquid ratio of 1:6, immerse and extract at 100°C for 90 minutes, continuously extract for 3 times, and obtain 5.2L of astragalus polysaccharide extract by suction filtration; add 23L, 90% ethanol was used for precipitation and separation, the precipitation time was 4 hours, and 450.6 g of crude product of astragalus polysaccharide was obtained by drying. Treat according to the method of preparing the standard curve, and measure the absorbance at 490nm. Use neutral protease to enzymolyze the crude astragalus polysaccharide at 55°C for 40 minutes, inactivate the enzyme in a boiling water bath for 7 minutes, and after cooling to room temperature, centrifuge to remove the denatured enzyme precipitate, and then adsorb on AB-8 macroporous adsorption resin with a flow rate of 2ml / min, adsorbed for 12 hours, and then eluted with 30% eth...

Embodiment 2

[0037] Preparation of Astragalus polysaccharides: Weigh 1kg of Astragalus membranaceus coarse powder, use n-butanol as a solvent to immerse and extract at 100°C for 90 minutes at a material-to-liquid ratio of 1:6, continuously extract 3 times, and filter to obtain 5.5 L of Astragalus polysaccharide extract; Add 27.5 L of 90% ethanol for precipitation and separation, the precipitation time is 4 hours, and dry to obtain 480.6 g of crude product of astragalus polysaccharide. Treat according to the method of preparing the standard curve, and measure the absorbance at 490nm. Use neutral protease to enzymolyze the crude astragalus polysaccharide at 40°C for 60 minutes, inactivate the enzyme in a boiling water bath for 9 minutes, cool to room temperature, centrifuge to remove the denatured enzyme precipitate, and then adsorb on AB-8 macroporous adsorption resin with a flow rate of 2ml / min, adsorbed for 12 hours, and then eluted with 30% ethanol at an elution flow rate of 2ml / min, an...

Embodiment 3

[0040] Preparation of Astragalus polysaccharides: Weigh 1kg of Astragalus membranaceus coarse powder, use n-butanol as a solvent to immerse and extract at 100°C for 90 minutes at a material-to-liquid ratio of 1:6, continuously extract 3 times, and filter to obtain 5.5 L of Astragalus polysaccharide extract; Add 27.5 L of 90% ethanol for precipitation and separation, the precipitation time is 4 hours, and dry to obtain 480.6 g of crude product of astragalus polysaccharide. Treat according to the method of preparing the standard curve, and measure the absorbance at 490nm. Use neutral protease to enzymatically hydrolyze the crude astragalus polysaccharide at 40°C for 60 minutes, inactivate the enzyme in a boiling water bath for 9 minutes, cool to room temperature, centrifuge to remove the denatured enzyme precipitate, and then adsorb on polyamide macroporous adsorption resin, and the column flow rate is 1ml / min , Adsorbed for 18h, then eluted with 40% ethanol at a flow rate of 1m...

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Abstract

The invention relates to a method for preparing polysaccharide selenide, and in particular relates to a method for preparing astragalus polysaccharide selenide by using an organic method. The method comprises the following steps of: performing enzymolysis on a crude astragalus polysaccharide product for 30 to 60 minutes at the temperature of between 40 and 60 DEG C, performing enzyme deactivation in a boiling water bath for 6 to 9 minutes, cooling to room temperature, centrifuging, removing denatured enzyme sediment, filling into a column, and evaporating eluent to obtain a pure astragalus polysaccharide product; performing selenylation on the pure astragalus polysaccharide product, namely adding the pure astragalus polysaccharide product into dehydrated pyridine, and stirring and scattering uniformly; dripping selenium oxychloride and succinic acid, fully stirring, adding a sodium nitrate solution containing 6 to 15 percent of sodium, and performing selenylation reaction for 12 to 20 hours at normal temperature to obtain suspension A; and adding sulfuric acid into the suspension A, settling, centrifuging, dialyzing, centrifuging the sediment by using absolute ethanol, performing vacuum freezing, and drying with carbon tetrachloride, thus obtaining powdery astragalus polysaccharide selenide. The method has the advantages of high yield of astragalus polysaccharide, low consumption of a selenylation agent, short selenylation reaction time, high selenium content, high stability and high biological value.

Description

technical field [0001] The invention relates to a preparation method of selenized polysaccharide, in particular to a method for preparing selenized astragalus polysaccharide by an organic method. Background technique [0002] In the past few decades, antibiotics as feed additives have played an important role in the prevention and control of livestock and poultry diseases and the improvement of animal production performance. However, the long-term use of antibiotic additives leads to drug resistance and drug residues in animal products directly affect human health through food residues and food chains, and will bring difficulties to human disease prevention and control. [0003] Astragalus is not only an immune enhancer and a good medicine with pharmacological effects such as sterilization and anti-virus, but also a feed additive for livestock and poultry that can promote growth and improve production efficiency. [0004] Astragalus (Astragalus) is a commonly used Chinese h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 姜礼辉邹晓庭
Owner 浙江博信药业股份有限公司