Induction and regeneration method for callus tissue of Capparis masaikai

A callus induction and callus technology, applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of staying in, difficult to meet the limitations of artificial planting and in-depth laboratory research, development and utilization, etc. , to achieve the effect of low production cost, great application value and solving the problem of seedling breeding

Inactive Publication Date: 2013-08-14
HAINAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic and foreign researches on betel nut are mainly in the aspects of component analysis and sweet protein gene, etc., followed by morphological research. There are few researches on the breeding of areca nut, and the breeding method is only in the state of seedling breeding. As a result, its development and utilization are greatly restricted, and it is difficult to meet the needs of artificial planting and in-depth research in the laboratory

Method used

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  • Induction and regeneration method for callus tissue of Capparis masaikai
  • Induction and regeneration method for callus tissue of Capparis masaikai
  • Induction and regeneration method for callus tissue of Capparis masaikai

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Experimental program
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Effect test

Embodiment 1

[0035] The method for inducing and regenerating the betel nut callus provided by the present embodiment comprises the following steps:

[0036] (1) Material selection and disinfection process: Select the immature fruit of betel nut that is free from diseases and insect pests and in good growth state, clean the fruit with clean water, carefully peel off the young immature embryo on the sterile workbench, and then place Disinfect in 75% alcohol by volume for 10 seconds, rinse with sterile water once, soak in 0.1% mercury chloride by mass for 3 minutes, wash with sterile water for 4 times, and shake it from time to time during soaking, so that The immature embryos of the explanted betel nut fully contacted with the disinfectant;

[0037] (2) Callus induction process: the immature embryos obtained in step (1) were cut into tissue pieces of about 2.0 cm and inoculated in callus induction medium, and cultured in dark at 26-28° C. for 45 days, Obtain the callus piece of the seed emb...

Embodiment 2

[0044] The method for inducing and regenerating the betel nut callus provided by the present embodiment comprises the following steps:

[0045] (1) Material selection and disinfection process: Select the immature fruit of betel nut that is free from diseases and insect pests and in good growth state, clean the fruit with clean water, carefully peel off the young immature embryo on the sterile workbench, and then place Disinfect in 75% alcohol by volume for 10 seconds, rinse with sterile water once, soak in 0.1% mercury chloride by mass for 3 minutes, wash with sterile water for 4 times, and shake it from time to time during soaking, so that The immature embryos of the explanted betel nut fully contacted with the disinfectant;

[0046] (2) Callus induction process: the immature embryos obtained in step (1) were cut into 1.5 cm tissue pieces and inoculated in callus induction medium, and cultured in the dark at 26-28°C for 35 days to obtain The seed embryo callus block of betel...

Embodiment 3

[0051] The method for inducing and regenerating the betel nut callus provided by the present embodiment comprises the following steps:

[0052] (1) Material selection and disinfection process: select naturally matured and dried seeds (that is, mature embryos), clean the seeds with clean water, carefully peel off the mature seed embryos on a sterile workbench, and then place them in a volume of 100 Disinfect in 70% alcohol for 60 seconds, rinse with sterile water once, soak in 0.1% mercury chloride for 20 minutes, wash with sterile water for 4 times, shake from time to time during soaking, so that the explants Full contact with disinfectant;

[0053] (2) Callus induction process: the mature embryos obtained in step (1) were cut into 1.5 cm tissue pieces and inoculated in callus induction medium, and cultured in dark at 26-28°C for 60 days to obtain The callus mass of betel nut seed embryos has a callus rate of 70%, and the induced callus is better, yellow, compact and shiny. T...

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Abstract

The invention discloses an induction and regeneration method for the callus tissue of Capparis masaikai. The induction and regeneration method is characterized by comprising the following steps of: (1) selection and disinfection for materials; (2) induction for the callus tissue; (3) induction for clustered buds; (4) proliferation culture for the clustered buds; and (5) rooting and seedling for adventitious buds. With the adoption of the method, the excellent characteristics of strains can be ensured, and lots of high-quality seedlings can be provided; and a good regeneration system method isprovided for large-area plantation, ecological and species protection, resource utilization and experiment for Capparis masaikai.

Description

technical field [0001] The invention relates to a method for cultivating plant tissue, in particular to a method for inducing and regenerating callus of caper nut. Background technique [0002] C. masaikai belongs to Capparidaceae (Capparidaceae), and is a plant of the genus Capparus; it is a perennial evergreen woody vine and climbing shrub, with a length of several meters to tens of meters; it is only distributed in tropical and subtropical regions of my country. Such as the high altitude (800-1600m) areas of provinces such as Yunnan, Guangxi and Guizhou; it is a rare and endangered wild fruit tree. [0003] Betel nut seeds can be used as medicine, its nature is bitter, sweet and cold, it can induce labor and contraception; it can clear away heat and detoxify, treat sore throat, malignant sores and swelling; In particular, its benevolent food has a long-lasting sweetness. Its seeds are rich in sweet protein. Among the seven kinds of plant sweet protein in the world, caper...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 胡新文于旭东吴繁花郭建春
Owner HAINAN UNIV
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