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Tissue culture method for lindera glauca

A tissue culture and mountain pepper technology, which is applied in the field of plant tissue culture, can solve the problems of long reproduction time, unutilized resources of good varieties of mountain pepper, low reproduction coefficient and the like

Inactive Publication Date: 2012-09-19
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the propagation of peppercorns mainly adopts conventional seed propagation and branch propagation, which takes a long time to reproduce and has a low reproduction coefficient, which cannot meet the increasing production and market demand, and many fine varieties of peppercorns cannot be used.

Method used

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  • Tissue culture method for lindera glauca
  • Tissue culture method for lindera glauca
  • Tissue culture method for lindera glauca

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Seed selection in the wild: choose mountain peppers that grow well and are free from diseases and insect pests in the Zhejiang Lin'an mountain pepper base.

[0041] (2) Obtaining sterile materials: cut the young axillary buds of the above-mentioned mountain pepper, wash them with tap water for 0.5h, and put them in an aqueous solution with a volume concentration of 1% detergent (transfer detergent) (volume ratio W / W) Shake for 2mins, rinse with sterile water. Soak in 70% ethanol aqueous solution with volume concentration for 30s on the ultra-clean workbench to sterilize the surface, then sterilize with 100ml of mixed disinfectant for 20mins (the mixed disinfectant is a mixture of 99.5ml of 84 disinfectant and 0.5ml of Tween 20 solution), after rinsing with sterile water for 3 times, the shoots were cut into 0.5 cm segments, and then the segments were inoculated into culture bottles containing cluster bud induction medium, and the bottle caps were sealed with a paraf...

Embodiment 2

[0045] The impact of different disinfectants of embodiment 2 on the growth of mountain pepper explants

[0046] 10% hydrogen peroxide (H 2 o 2 ) aqueous solution, mercuric chloride (HgCl) with a mass concentration of 0.1% 2 ) aqueous solution and the mixed disinfectant solution in Example 1 (the mixed disinfectant solution is the mixed solution of 84 disinfectant solution of 99.5ml and Tween 20 of 0.5ml) to disinfect the explant material (i.e. the axillary bud) of the peppercorns, Other operations are the same as in Example 1. The segments were respectively inoculated into culture bottles filled with cluster bud induction medium, and the bottles were sealed with a parafilm and then placed in an incubator. After cultivating for 15 days at 24° C. under the condition of 1500 lx light, observe and count the effects of different disinfectants on the induction and growth of peppercorn buds. The results are shown in Table 1. The results showed that the mixed disinfectant and mass...

Embodiment 3

[0049] Example 3 The Induction Effect of Different Hormone Combinations on Mountain Pepper Buds

[0050] The axillary buds of Japonica japonica were respectively inoculated in the MS basic medium (namely the medium for clustering bud induction) added with different concentrations of 6-BA and NAA, and the other operations were the same as in Example 1. The results showed that the medium with different hormone combinations could affect the bud formation to varying degrees, but the concentration of the hormone ratio was too high or too low, which was unfavorable to the induction of the pepper buds, and yellow and white buds, rooting and vitrification would appear. Among them, medium MS+6-BA2.0mg / L+NAA 0.3mg / L is the best for the induction of mountain pepper buds, and the most regenerated buds are induced, with green buds, fast growth, large and strong buds, no rooting and Vitrification phenomenon, the average bud length is 1.75cm, and the bud induction rate reaches 100%.

[0051...

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Abstract

The invention discloses a tissue culture method for lindera glauca. With the young, tender axillary buds of lindera glauca as explants, a rapid in vitro propagation method based on tissue culture, which includes obtainment of aseptic materials, induced differentiation and multiplication of adventitious buds, rooting of the buds, transplantation and the like, is established. The tissue culture method improves culture medium, adds glutamine regulator, greatly increases the differentiation rate, leaves out the aseptic induced rooting step in tissue culture, adopts open operation, and utilizes NAA to induce rooting, so that complete plants are grown; and the method overcomes the defects of long conventional lindera glauca-breeding period, low propagation coefficient and the like, enhances thestability of the trait of lindera glauca, can realize the factory mass production of seedlings, and can effectively solve the problem on how to supply high-quality lindera glauca seedlings.

Description

(1) Technical field [0001] The invention relates to the technical field of plant tissue culture, and relates to a method for tissue culture and rapid seedling propagation of mountain pepper. By this method, large-scale production of fine seedlings can be carried out. (2) Background technology [0002] Litsea pepper (Lindera glauca) belongs to the Lauraceae plant, deciduous shrub or small tree, also known as cubeb, wild pepper, lizard pepper, mountain pepper, wood ginger and so on. Mainly distributed in Shandong, Anhui, Zhejiang, Jiangxi, Fujian, Taiwan, Henan, Hunan, Guangdong, Guangxi, Sichuan, Yunnan and other places. Mountain pepper can not only be used as a decorative tree species in gardens, but also can be planted in lawns, flower beds and rockery gaps, and the fruit and leaves of mountain pepper are fragrant, and aromatic oil can be extracted from the fruit and leaves, which can be used as food and cosmetic essence. The seeds contain fatty oil and can be used to make...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 邹克琴李素芳王为民徐晓晖刘军
Owner CHINA JILIANG UNIV
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